Follicle Kinetics in the Ovary of the Cyclic Mouse

Pedersen, Tore

doi:10.1530/acta.0.0640304

Cited by 205 publications

(112 citation statements)

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“…Pedersen (1972), using the pulse-labelling technique, estimated that in mature mice 10-20 follicles per day begin rapid growth. He has also provided estimates of the time intervals involved in follicular growth in the mouse (Pedersen 1970).…”

Section: Discussionmentioning

confidence: 99%

“…However, as each specimen represents only one point in time, it is difficult to obtain a true picture of the dynamics offollicular growth and regression. Using the mouse, this difficulty has been circumvented by pulse-labelling of the granulosa with tritiated thymidine and removing the ovaries at set intervals thereafter (Peters and Levy 1966;Pedersen 1970Pedersen , 1972. This method was attempted with sheep but abandoned because of expense and practical difficulties.…”

Section: Introductionmentioning

confidence: 99%

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The Pattern of Follicular Growth and Atresia in the Ovine Ovary

Turnbull¹,

Braden²,

Mattner³

1977

Aust. Jnl. Of Bio. Sci.

153

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The ovaries of 21 2-year-old Merino ewes at various stages of the oestrous cycle were serially sectioned and all follicles > 0·5 mm in diameter were counted and measured. The mean number of normal follicles > 0·5 mm in diameter was 19· 9 per ewe, of which 8·7 were > 1 mm in diameter. The mean numbers of follicles > 0·5 mm in diameter in 'early', 'advanced' and 'late' stages of atresia were 4· 6, 9· 0 and 12· 3 respectively. Early atresia was rarely seen in follicles < 1 mm in diameter; the greatest incidence was in follicles of diameter 1· 5-2' 5 mm.Volumes of the granulosa, theca interna and antrum were calculated for all follicle sizes and the mitotic index of the granulosa and theca determined. Follicles were classified according to granulosa volume, each class having twice the volume of the preceding class. Estimates of the doubling time of the granulosa were made from the mitotic index and an estimate of mitotic time (0'43 h) obtained by use of colchicine on two other ewes. In all ewes, irrespective of day of oestrous cycle, the rate of growth was slow in follicles < 0·4 mm in diameter; above this size it accelerated to a maximum (doubling time 26-30 h) in follicles O· 7-2 mm in diameter. It was estimated that, on average, a follicle takes about 5 days to grow from 0·5 to 2·2mm diameter, and a further 4 days to reach 4·5 mm diameter. The number of follicles entering the rapid growth phase was estimated to be 3-4 per day. The present data do not support any particular 'wave' theory of follicular development, but indicate that follicles are growing or regressing asynchronously at any given time during the luteal stage of the oestrous cycle.At 48 h after treatment of two ewes with PMS gonadotrophin (PMSG), the number of follicles > 0·5 mm in diameter was increased, the greatest proportional increase being in those > 3·5 mm in diameter. Relatively few follicles were undergoing early atresia. At 18 h after RCG treatment (given 48 h after PMSG to two ewes) all follicles> 3·5 mm in diameter were undergoing luteinization and two-thirds of the follicles between 1 and 3·5 mm in diameter were undergoing early atresia.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Pattern of Follicular Growth and Atresia in the Ovine Ovary

Turnbull¹,

Braden²,

Mattner³

1977

Aust. Jnl. Of Bio. Sci.

153

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“…The luteinized granulosa cells of the corpus luteum and corpus albicans, as well as advanced atretic follicles, appear to be preferentially affected, with the stroma following in sensitivity. Healthy follicles of type 5a (Pedersen, 1970) and below, thecal cells, and the germinal epithelium appear to be quite resistant in all but the most severely damaged areas. The healthy oocyte, i.e.…”

Section: Observationsmentioning

confidence: 98%

Ultrasonically induced morphological damage to mouse ovaries

Bailey

O’Brien

Dunn

1983

Ultrasound in Medicine & Biology

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Abstract-Mouseovaries were exposed in uiuo to I MHz continuous wave ultrasonic energy at spatial peak intensities ranging from 5 to 100 W/cm' for times varying from 300 to 15 s depending on the intensity. Following exposure the ovaries were surgically removed at times ranging from immediately (within 60 s) to 7 days and prepared histologically for light microscopic analysis. The observed tissue alterations varied from severe, at the higher intensities to subtle, at the lower intensities. Lesions were manifested by pyknosis of cells, vacuolization of cells and tissue, eosinophilic cytoplasm, and general cellular disruption. Subtle alterations showed large numbers of polyovular follicles and increases in the amount of PAS positive material in the interstitial tissue. Various ovarian structures showed differing sensitivities to the insult with luteinized structures being preferentially altered.

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“…The aim of the present experiments was to combine ink labelling with histology to study the dynamics of preovulatory enlargement during the follicular phase of the oestrous cycle in three prolific breeds or strains of sheep (Romanov, Finnish (Driancourt & Cahill, 1984 (Driancourt et al, 1985a), to estimate accurately the number of granulosa cells in a sample of preovulatory follicles, the diameter, thickness and number of granulosa cells were measured in the histological section with the largest diameter and calculations made according to Pedersen (1970) and Gougeon (1981 (Siegel, 1956). Distributions were compared by 21 tests (Sokal & Rohlf, 1969 (Fig.…”

Section: Introductionmentioning

confidence: 99%