Ultrasonically induced morphological damage to mouse ovaries

Bailey, K. I.; O’Brien, William D.; Dunn, F.

doi:10.1016/0301-5629(83)90106-0

Cited by 9 publications

(3 citation statements)

References 4 publications

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“…Similar findings were also reported in ovarian follicles undergoing natural [17] or induced [18] atresia. Moreover, degeneration and detachment of granulosa cells during atresia are related to apoptosis, and according to Hughes and Gorospe [19], DNA cleavage begins before any morphological signs of cellular death.…”

Section: Discussionsupporting

confidence: 88%

Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles

et al. 2004

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Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian folliclesThe maintenance of follicle quality during the transportation of ovaries is essential for the successful cryopreservation and in vitro development of preantral follicles. The objective of this study was to evaluate the effect of cooling ovarian tissue on the conservation of zebu cow preantral follicles. Ovarian pieces were immersed in saline or coconut water (CW) solutions and maintained at 4 or 20 °C for 6, 12, or 18 h. Preantral follicles were evaluated by histology and transmission electron microscopy. Storage of ovarian pieces at 20 °C for 12 or 18 h significantly reduced the percentage of morphologically normal follicles compared to controls. In contrast, conservation at 4 °C for up to 18 h and at 20 °C for up to 6 h kept the percentage of normal follicles similar to controls. However, the type of solution that the ovaries were immersed in had little effect on the results. Decreased cellular metabolism probably accounted for better preservation of preantral follicles at 4 °C. In conclusion, zebu cow ovaries were successfully stored at 4 °C for up to 18 h with no morphological damage to preantral follicles. However, at 20 °C, ovaries could only be stored for 6 h.

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Section: Discussionsupporting

confidence: 88%

Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles

et al. 2004

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“…For this reason, as well as the variety of wounds that occur from the heterogeneous effects of ultrasound exposure (Bailey et al 1983, Keyhani et al 2001), we feel this method is a good model for producing a large spectrum of wound types, leading to multiple death modes that can be analyzed using high resolution microscopy and flow cytometry. We also utilized more direct methods for creating wounds, i.e., mechanical shear, and laser ablation, which may mimic some effects of ultrasound on cells (Dunn 1985) while creating more homogenous outcomes than ultrasound exposure, but these methods are likely to be less useful for future clinical applications.…”

Section: Introductionmentioning

confidence: 99%

“…Ultrasound exposure can also result in cellular and tissue damage (Alter et al 1998, Bailey et al 1983) and cell death via routes including both necrosis and apoptosis (Cochran and Prausnitz 2001, Feril and Kondo 2004, Miller and Dou 2009, Miller and Quddus 2002). …”

Section: Introductionmentioning

confidence: 99%

Changes in Cell Morphology Due to Plasma Membrane Wounding by Acoustic Cavitation

Schlicher

Hutcheson²,

Radhakrishna

et al. 2010

Ultrasound in Medicine & Biology

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Acoustic cavitation-mediated wounding (i.e., sonoporation) has great potential to improve medical and laboratory applications requiring intracellular uptake of exogenous molecules; however, the field lacks detailed understanding of cavitation-induced morphological changes in cells and their relative importance. Here, we present an in-depth study of the effects of acoustic cavitation on cells using electron and confocal microscopy coupled with quantitative flow cytometry. High resolution images of treated cells show that morphologically different types of blebs can occur after wounding conditions caused by ultrasound exposure as well as by mechanical shear and strong laser ablation. In addition, these treatments caused wound-induced non-lytic necrotic death resulting in cell bodies we call wound-derived perikarya (WD-P). However, only cells exposed to acoustic cavitation experienced ejection of intact nuclei and nearly instant lytic necrosis. Quantitative analysis by flow cytometry indicates that wound-derived perikarya are the dominant morphology of nonviable cells, except at the strongest wounding conditions, where nuclear ejection accounts for a significant portion of cell death after ultrasound exposure.

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Effects of ultrasound on ovulation in the mouse

et al. 1989

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In vitro fertilization and embryo transfer (IVF-ET) programs use ultrasound extensively for monitoring the growth of ovarian follicles and, subsequently, for confirming the presence of a fetal sac. There have been few reports of the effects of ultrasound on ovulation rates in mammals, and we report here that following exposure to continuous wave ultrasound at a spatial average intensity of 3.0 W/cm2 for five minutes, ovulation rates measured 10 days later were significantly reduced in mice. When temperature elevation of the exposed ovary was measured with a thermocouple, hyperthermia correlated with reduction in ovulation.

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Ultrasonically induced morphological damage to mouse ovaries

Cited by 9 publications

References 4 publications

Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles

Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles

Changes in Cell Morphology Due to Plasma Membrane Wounding by Acoustic Cavitation

Effects of ultrasound on ovulation in the mouse

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