Follicle Stimulating Hormone Increases Somatic and Germ Cell Number in the Ovary during Chick Embryo Development

Méndez-Herrera, M.C.; Tamez, L.; Cândido, Alexandre Luna; Reyes‐Esparza, Jorge; Pedernera, E.

doi:10.1006/gcen.1998.7108

Cited by 20 publications

(7 citation statements)

References 33 publications

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“…The available experimental data suggest that estrogens could be involved in germ cell proliferation (Scheib 1983;Méndez et al 1993Méndez et al , 1998. Therefore, evidence of ER expression in oocytes would strongly support the possibility of estrogen action on the germ cell subpopulation.…”

Section: Introductionmentioning

confidence: 92%

Evidence for estrogen receptor expression in germ cell and somatic cell subpopulations in the ovary of the newly hatched chicken

Méndez

Chávez

Echeverría

et al. 1999

Cell and Tissue Research

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Estrogens are involved in the gonadal morphogenesis of vertebrates, and almost all hormonal effects of 17beta-estradiol are mediated through specific receptors. At the time of sexual differentiation in the chicken, or even before, there is evidence of the presence of estrogen receptors and the secretion of 17beta-estradiol. However, no information is available regarding the cellular types that express the estrogen receptor in the immature chick ovary. The present study analyzes estrogen receptor expression in germ and somatic cells of the ovary in the newly hatched chicken. Highly purified cell subpopulations of germ and somatic cells were evaluated for specific 17beta-estradiol nuclear binding. In addition, the estrogen receptor was localized at the ultrastructural level by the immunogold technique. Finally, reverse transcription and polymerase chain reaction procedures detected a steady-state level of mRNA for the estrogen receptor. Somatic cells including typical steroidogenic cells showed specific 17beta-estradiol nuclear binding, displayed the estrogen receptor, and possessed estrogen receptor transcripts. The same result was observed in primary oocytes, together with the ultrastructural localization of estrogen receptor in extended chromatin filaments. Our experimental data support the hypothesis that estrogens are involved in the function of somatic and germ cells subpopulations in the immature chicken ovary.

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Section: Introductionmentioning

confidence: 92%

Evidence for estrogen receptor expression in germ cell and somatic cell subpopulations in the ovary of the newly hatched chicken

Méndez

Chávez

Echeverría

et al. 1999

Cell and Tissue Research

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“…Owing to the effects of gonadtropins on cell proliferation in the gonads of chickens during embryogenesis (Pedernera et al ., 1999;Méndez-Herrera et al ., 1998), it is important to clarify the temporal properties of differentiation of gonadotropes during chicken pituitary gland development. Although several immunohistochemical studies have shown the ontogeny of chicken gonadotropes during embryogenesis, there is no agreement concerning the temporal and spatial appearance of gonadotropes (Mikami and Takahashi, 1987;Puebla-Osorio et al ., 2002).…”

Section: Introductionmentioning

confidence: 99%

Development of Gonadotropes in the Chicken Embryonic Pituitary Gland

et al. 2004

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“…We examine both basal and FSH-stimulated levels of circulating E 2 and testosterone (T) and gonadal mRNA expression levels of Inha, Inhba, Fst , aromatase ( Cyp19a1 ), and Fshr . Comparatively, ovaries of both embryonic and hatchling chicken are responsive to exogenous FSH, both in vitro (Pedernera et al, '99) and in vivo (Gonzalez-Moran, '98; Mendez-Herrera et al, '98; Sanchez-Bringas et al, 2006), resulting in elevated circulating estradiol and ovarian cell proliferation. Furthermore, in cultured chicken granulosa, activin signaling is necessary to maintain morphological differentiation (Schmierer et al, 2003), whereas FSH increases expression of Inha, Fst , and Inhba mRNA (Davis et al, 2001; Safi et al, 2003).…”

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confidence: 99%

Altered sex hormone concentrations and gonadal mRNA expression levels of activin signaling factors in hatchling alligators from a contaminated Florida lake

et al. 2010

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Activins and estrogens participate in regulating the breakdown of ovarian germ cell nests and follicle assembly in mammals. In 1994, our group reported elevated frequencies of abnormal, multioocytic ovarian follicles in 6 month old, environmental contaminant-exposed female alligators after gonadotropin challenge. Here, we investigated if maternal contribution of endocrine disrupting contaminants to the egg subsequently alters estrogen/inhibin/activin signaling in hatchling female offspring, putatively predisposing an increased frequency of multioocytic follicle formation. We quantified basal and exogenous gonadotropin-stimulated concentrations of circulating plasma steroid hormones and ovarian activin signaling factor mRNA abundance in hatchling alligators from the same contaminated (Lake Apopka) and reference (Lake Woodruff) Florida lakes, as examined in 1994. Basal circulating plasma estradiol and testosterone concentrations were greater in alligators from the contaminated environment, whereas activin/inhibin βA subunit and follistatin mRNA abundances were lower than values measured in ovaries from reference lake animals. Challenged, contaminant-exposed animals showed a more robust increase in plasma estradiol concentration following an acute follicle stimulating hormone (FSH) challenge compared with reference site alligators. Aromatase and follistatin mRNA levels increased in response to an extended FSH challenge in the reference site animals, but not in the contaminant-exposed animals. In hatchling alligators, ovarian follicles have not yet formed; therefore, these endocrine differences are likely to affect subsequent ovarian development, including ovarian follicle assembly.

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Follicle Stimulating Hormone Increases Somatic and Germ Cell Number in the Ovary during Chick Embryo Development

Cited by 20 publications

References 33 publications

Evidence for estrogen receptor expression in germ cell and somatic cell subpopulations in the ovary of the newly hatched chicken

Evidence for estrogen receptor expression in germ cell and somatic cell subpopulations in the ovary of the newly hatched chicken

Development of Gonadotropes in the Chicken Embryonic Pituitary Gland

Altered sex hormone concentrations and gonadal mRNA expression levels of activin signaling factors in hatchling alligators from a contaminated Florida lake

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