Follicular fluid 8-Hydroxy-2-Deoxyguanosine (8-OHdG) as biomarker for oxidative stress in intracytoplasmic sperm injection

Abstract

Oxidative stress (OS) is a situation that occurs as a result of un-equilibrium between reactive oxygen species (ROS) formation and the antioxidant defence system. 8-hydroxy-2-deoxyguanosine (8-OHdG), an oxidized form of deoxyguanosine, is found in higher levels in aging oocytes. In this cross-sectional study, we aimed to evaluate the effect of 8-OHdG on the intracytoplasmic sperm injection (ICSI). Follicular fluid (FF) samples were taken after removing the oocyte for later analysis of 8-OHdG. The couples participants were categorized according to the cause of subfertility into three groups (female factor, malefactor, and unexplained infertility). Further division according to pregnancy state was done to evaluate the precise role of 8-OHdG on pregnancy state and ICSI outcome. Result : This study showed that the 8-OHdG levels significantly higher in non-pregnant women (p < 0.05). Correlation study showed that 8-OHdG level in follicular fluid is negatively correlating with the number of retrieved oocytes, metaphase two oocytes (MII), fertilized oocytes (2PN = two pronuclei), cleaved zygotes, good quality embryos, and the difference was statistically significant (p < 0.05). Conclusion: The study revealed that the 8-OHdG level in follicular fluid negatively influences ICSI outcome and it is higher in non-pregnant women.

Ginkgolide A attenuated apoptosis via inhibition of oxidative stress in mice with traumatic brain injury

“…As is well known, inhibiting oxidative stress and cell apoptosis are some potential mechanisms of anti-ischemic drugs, and ginkgolides can achieve this [ 35 ]. We present found that 8-OHdG, a biomarker of oxidative stress [ 36 ], raised in TBI mice, and was inhibited by GA administration. After treatment of GA, the increase of MDA and the decrease of SOD in the brain of TBI were also reversed.…”

Current Understanding of and Future Directions for Endometriosis-Related Infertility Research with a Focus on Ferroptosis

“…As follicular fluid is closely associated with granulosa cells and oocytes, iron-or oxidative stress-induced proteins (e.g., MDA, 8-OHdG, and ferroportin) may serve as biomarkers for evaluating oocyte quality [73]. Accumulating evidence suggests that the 8-OHdG level in follicular fluid is elevated in women with endometriosis [50] or negatively influences ICSI outcome [99]. The use of such markers in the follicular fluid may afford opportunities to predict and assess infertility outcomes.…”

OXIDATIVE STRESS AND REPRODUCTIVE FUNCTION: The impact of oxidative stress on reproduction: a focus on gametogenesis and fertilization

“…The development of probes to detect DNA oxidation in the form of 8OHdG has also been used as an oxidative stress biomarker in a reproductive context. For example, the ability of xenobiotics to induce oxidative stress during pregnancy (Lan et al 2022), to monitor the quality of oocytes used in ICSI cycles (Mukheef et al 2022), to evaluate oxidative stress in spermatozoa (Vorilhon et al 2018, Gharagozloo et al 2022, and as a means of determining the reproductive toxicity associated with a wide variety of pollutants including phthalate esters and insecticides (Brassea-Pérez et al 2022, Pan et al 2022.…”