Follicular fluid progesterone downregulated HPGD and COX2 in granulosa cells via suppressing NF-ĸB in endometriosis

Li, Jingyi; Chen, Jianpeng; Qian, Yu-Li; Ma, Junyan; Ni, Fei-Da; Lin, Yifeng; Zhang, Runju; Ying, Yue; Zhang, Yanye; Wang, Siwen; Huang, Yun; Liu, Juan; Tu, Mixue; Ying, Yiqun; Wu, Yiqing; Wu, Xuechen; Wu, Biao; Zhu, Bo; Zhang, Dan

doi:10.1093/biolre/ioad014

Cited by 7 publications

(4 citation statements)

References 35 publications

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“…Effects of Glu and INS on GDM of two study groups up-regulated in FF of endometriosis patients[20]. Those data suggested dysregulated circulating metabolic molecules might play an important role in endometriosis development.…”

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confidence: 75%

Effects of dysregulated glucose metabolism on the occurrence and ART outcome of endometriosis

Chen

Zhang

Jin

et al. 2023

Preprint

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Background: Endometriosis can affect the systemic metabolic indicators, including body mass index, glucose metabolism and lipid metabolism, while the association between metabolic indexes and the occurrence and ART outcome of endometriosis is unclear. We aimed to evaluate the characteristics of systemic metabolic indexes of endometriosis patients with infertility and their effects on pregnancy outcome after ART treatment. Methods:A retrospective cohort study involve 412 endometriosis patients and 1551 controls was conducted. Primary outcome was metabolic indexes, and secondary measures consisted of the influence of metabolic indexes on the number of retrieved oocytes and ART outcomes, as well as the relation between blood glucose and prevalence of GDM. Results:Endometriosis patients had higher INS [6.90(5.10-9.50) vs 6.50(4.80-8.90) μU/mL, P = 0.005]. A prediction model for endometriosis combining the number of previous pregnancies, CA125, fasting blood Glu and INS, had a sensitivity of 73.9%, specificity of 67.8% and area AUC of 0.77. There were no significant differences in ART outcomes and complications. The levels of Glu were associated with GDM both in endometriosis group (aOR 12.95, 95% CI 1.69–99.42; P = 0.014) and in control group (aOR 4.15, 95% CI 1.50–11.53; P = 0.006). Conclusions: Endometriosis patients had abnormal metabolic indexes, in which serum Glu and serum INS are related to the occurrence of endometriosis, the number of retrieved oocytes and the occurrence of GDM. A prediction model based on metabolic indexes was established, representing a promising non-invasive method to predict endometriosis patients with known pregnancy history.

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confidence: 75%

Effects of dysregulated glucose metabolism on the occurrence and ART outcome of endometriosis

Chen

Zhang

Jin

et al. 2023

Preprint

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“…Both estrogen and progesterone levels considerably reduced following endometriotic fluid administration which could account for the observed drop in the diestrus phase and compromise in estrous cycle regularity. 25,26 Several studies looking at women with endometriotic have demonstrated that high LH secretion facilitates overproduction of ovarian androgens, which in turn is connected to heightened pituitary sensitivity to GnRH. Our study demonstrates that 2 mL/kg of endometriotic follicular fluid significantly increases LH levels.…”

Section: Discussionmentioning

confidence: 99%

Systemic Effect of Human Follicular Fluid from Endometriotic and Healthy Subjects on Female Mice

Gairola,

Chitme,

Sircar

2024

Ind. J. Pharm. Edu. Res

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Aim/Background: Throughout oogenesis and folliculogenesis, the follicular fluid's composition alters physiologically to fit the needs of particular microenvironmental demands. This study's main goal was to compare the effect of follicular fluid collected from endometriotic and non-endometriotic patients on systemic body functioning of female mice. Materials and Methods: Both healthy and endometriotic participants' follicular fluid was collected and pooled separately. Female Swiss albino mice were injected with 1 and 2 mL/kg/day endometrial fluid in the intraperitoneal region and monitored for 21 days. Change in body weight, hormonal profile, glucose profile and hematological profile was monitored and recorded on regular basis. Results: On day 21, the blood glucose level increased from 100.2±0.96 mg/dL to 138.4±3.32 mg/dL. Endometriotic follicular fluid had a dose-dependently decreased serum estradiol from 28.80±0.37 to 27.00±1.0 ng/mL and progesterone form 24.17±0.7 to 1.72±0.21 pg/mL and a rise in testosterone levels from normal 3.95±0.81 nmol/mL to 9.4±0.92. It has elevated serum LH levels to approximately three times normal levels. In contrast, the serum FSH level decreased from 19.40±0.74 mIU/mL to 2.5±0.22. As a result, the LH to FSH ratio increased from 0.18±0.01 to 3.9±0.19. There was a dose-dependent rise in serum insulin level significantly (p<0.001) from normal 0.74±0.02 IU/mL to 1.63±0.05 and 2.09±0.1 respectively. In a similar manner, HOMA-IR also showed increase in insulin resistance from normal 0.17 to 0.57±0.02 and 0.71±0.04. HOMA-Beta normal level was 8.17±0.3 increased to 10.05±0.4 beta cell dysfunction. Nevertheless, QUICKI were both dosages dependently decreased insulin sensitivity from 1.31±0.07 dose dependently to 0.9±0.01and 0.83±0.02. Conclusion: Female mice treated with endometriotic follicular fluid of endometriosis patients displayed pancreatic abnormality. It has been concluded that endometriotic patients' follicular fluid not only has a localized effect but also contains elements that enter the systemic circulation, have negative effects, and may be connected to significant clinical symptoms of endometriotic condition.

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“…Recently, metabolic approach has emerged as a possible non-invasive diagnostic tool in women with or without endometriosis [ 18 – 23 ]. Our previous study showed that most metabolites important for glucolipid metabolism were up-regulated in follicular fluid (FF) of endometriosis patients [ 24 ]. Those data suggested dysregulated circulating metabolic molecules might play an important role in endometriosis development.…”

Section: Discussionmentioning

confidence: 99%

Effects of dysregulated glucose metabolism on the occurrence and ART outcome of endometriosis

Chen,

Zhang,

Jin

et al. 2023

Eur J Med Res

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Background Endometriosis is associated with systemic metabolic indicators, including body mass index (BMI), glucose metabolism and lipid metabolism, while the association between metabolic indexes and the occurrence and assisted reproductive technology (ART) outcome of endometriosis is unclear. We aimed to evaluate the characteristics of systemic metabolic indexes of endometriosis patients with infertility and their effects on pregnancy outcome after ART treatment. Methods A retrospective cohort study involve 412 endometriosis patients and 1551 controls was conducted. Primary outcome was metabolic indexes, and secondary measures consisted of the influence of metabolic indexes on the number of retrieved oocytes and ART outcomes. Results Endometriosis patients had higher insulin (INS) [6.90(5.10–9.50) vs 6.50(4.80–8.90) μU/mL, P = 0.005]. A prediction model for endometriosis combining the number of previous pregnancies, CA125, fasting blood glucose (Glu) and INS, had a sensitivity of 73.9%, specificity of 67.8% and area under curve (AUC) of 0.77. There were no significant differences in ART outcomes and complications during pregnancy. The serum levels of Glu before pregnancy were associated with GDM both in endometriosis group (aOR 12.95, 95% CI 1.69–99.42, P = 0.014) and in control group (aOR 4.15, 95% CI 1.50–11.53, P = 0.006). Conclusions We found serum Glu is related to the number of retrieved oocytes in control group, serum INS is related to the number of retrieved oocytes in endometriosis group, while serum Glu and INS before pregnancy are related to the occurrence of GDM in two groups. A prediction model based on metabolic indexes was established, representing a promising non-invasive method to predict endometriosis patients with known pregnancy history.

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Follicular fluid progesterone downregulated HPGD and COX2 in granulosa cells via suppressing NF-ĸB in endometriosis

Cited by 7 publications

References 35 publications

Effects of dysregulated glucose metabolism on the occurrence and ART outcome of endometriosis

Effects of dysregulated glucose metabolism on the occurrence and ART outcome of endometriosis

Systemic Effect of Human Follicular Fluid from Endometriotic and Healthy Subjects on Female Mice

Effects of dysregulated glucose metabolism on the occurrence and ART outcome of endometriosis

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