Follicular growth and estradiol follow-up after subcutaneous xenografting of fresh and cryopreserved human ovarian tissue

Schubert, B.; Canis, Michel; Darcha, Claude; Artonne, Christine; Smitz, Johan; Grizard, G.

doi:10.1016/j.fertnstert.2007.03.101

Cited by 61 publications

(49 citation statements)

References 55 publications

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“…This evidence may explain that, although there was no functional graft damage, the fresh graft implant presented better results, according to the parameters analyzed in the vaginal smears. Schubert et al 12 reinforce this hypothesis because, based on histological analysis, they suggest that cryopreserved tissue follicles need a longer time to mature. Similar considerations were made by Choi et al 13 that cryopreservation may delay the development of preantral follicles due to the suppression of cellular proliferation by PCNA on the granulose layer and the increase of cellular necrosis in these follicles.…”

Section: Discussionmentioning

confidence: 94%

Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning

et al. 2009

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Purpose:To evaluate the remote ischemic preconditioning (R-IPC) impact on the quality of the ovarian graft by means of vaginal smear of transplanted rats. Methods: Sixty rats were used divided in six groups: Control; Fresh transplant (TxF); Cryopreserved transplant (TxC); R-IPC; R-IPC + fresh transplant (TxF+R-IPC); R-IPC + cryopreserved transplant (TxC+R-IPC). R-IPC was performed in the common iliac artery. Autologous ovarian tissue was implanted integrally in the retro peritoneum. On the first PO day, vaginal smear collection was daily initiated. After 30 days, the PO day when the estrous cycle was re-initiated was considered for analysis as well as the estrous days and the number of estrous cycles. Results: R-IPC showed a tendency to an early estrus re-initiation (p>0.05) as well as increase the number of cycles in the fresh transplanted group while in the cryopreserved transplant the number of cycles was similar, regardless of the stimulus R-IPC (p>0.05). The animals which had undergone fresh grafts had a longer estrous period than the ones which had undergone cryopreserved grafts, with or without R-IPC (p<0.05). Conclusion: R-IPC promoted earlier re-initiation of ovarian activity in the PO and greater estrous frequency, with more consistent results in the fresh grafts than in the cryopreserved ones.

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Section: Discussionmentioning

confidence: 94%

Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning

et al. 2009

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“…Moreover, antioxidant supplements such as taurine and L-glutamine in freezing media have been found to reduce cryoinjuries [4,40]. In an earlier study, follicular growth was observed after subcutaneous xenografting of human ovarian tissue cryopreserved according an original protocol using a serumfree freezing medium supplemented with PrOH and raffinose as well as antioxidants, and a faster freezing program than the usual one [15,39]. Nevertheless, further studies of the quality and functionality of thawed ovarian tissue are needed to support the feasibility of this freezing protocol in clinical settings.…”

Section: Introductionmentioning

confidence: 99%

Quality and functionality of human ovarian tissue after cryopreservation using an original slow freezing procedure

Sanfilippo

Canis

Romero

et al. 2012

J Assist Reprod Genet

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Purpose To evaluate the efficiency of an original slow freezing protocol on the quality and function of human ovarian cortex. Methods Human ovarian tissues were cryopreserved using a freezing medium supplemented with propanediol and raffinose as cryoprotectants and antioxidants (L-glutamine, taurine). Samples were then frozen using a faster cooling rate than the usual one. Viability and morphology of follicles, DNA fragmentation in follicles and stroma as well as histology of the vascular endothelium were analyzed before and after freezing/thawing. Moreover, a functional analysis was performed based on the evaluation of follicular growth and development in thawed ovarian tissues that were cultured in vitro. Results Our freezing/thawing protocol allows preservation of a high proportion of viable follicles and the preservation of the different follicle developmental stages (p>0.05 versus fresh control). 70.5±5.2 % of follicles retained an intact morphology after cryopreservation (p=0.04). Stroma cells but not follicles exhibited a slight increase of DNA fragmentation after thawing (p<0.05). Microvessel endothelium within thawed tissues appeared to be preserved. Granulosa cells showed signs of proliferation in follicles cultured for 12 days. Secretion of 17β-oestradiol significantly increased during in vitro culture. Conclusions This protocol leads to good preservation of ovarian integrity and functionality post-thawing and thus appears as a suitable technique of ovarian tissue cryopreservation in clinical settings. Further research could be extended to optimize conditions of in vitro culture.

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“…Immunotolerance was also not induced between mouse strains (Gosden 2007(Gosden , 2008. So far, in order to facilitate these experiments, most studies have used immunodeficient or inbred mice as recipients to reduce/eliminate rejection after ovarian transplantation (Candy et al 2000, Hosoe et al 2008, Schubert et al 2008, Soleimani et al 2008, Abir et al 2009, Motohashi et al 2009). In these cases, athymic nude mice or severe combined immunodeficient mice (who exhibition absence of mature B and T cells) were used to prevent immune rejection.…”

Section: Introductionmentioning

confidence: 99%

“…Renal envelopes were highly preferred in the past, and the transplanted ovaries developed well there (Carroll et al 1990, Waterhouse et al 2004. In recent years, subcutaneous and muscle tissues have become the alternative sites for ovary implants (Oktay et al 2003, Lee et al 2004, Yang et al 2006, Schubert et al 2008, Soleimani et al 2008, Eimani et al 2009). Ovarian transplantation under the kidney capsule requires a more difficult surgical operation, and the development of grafts cannot be observed during the process.…”

Section: Introductionmentioning

confidence: 99%

Follicle growth and oocyte development after ovary transplantation into back muscle of immune-intact adult castrated male mice

Li¹,

Tao²,

Zhang³

et al. 2010

Reproduction

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Ovary grafting is not only a method of investigating follicle and oocyte development, but also a useful model to explore the possibility of the re-establishment of the reproductive axis in male-to-female sexual reversal. This study investigated ovary survival and follicle development after mouse ovaries were transplanted into immune-intact castrated male mice. Ten-day-old mouse ovaries were transplanted into the back muscle of adult outbred castrated male mice treated with immunosuppressants. Twenty-two days later, the ovary structure and the number of follicles present was examined by hematoxylin and eosin staining. The oocytes were harvested, and then used for in vitro maturation (IVM) and IVF. The results showed that primordial and antral follicles were mainly found in the grafts, and there were obvious differences compared with 32-day-old fresh ovaries (P!0.05). Embryos were derived from collected oocytes after IVM and IVF with a 72.4% cleavage rate and 7.9% blastocyst rate; 12 live pups were generated by embryo transfer. The hormone assay showed that plasma concentrations of both estrogen and progesterone increased after ovarian transplantation (P!0.01). In conclusion, immune-intact adult castrated male mice can support ovary survival and further development of follicles with endocrine function after ovarian transplantation.

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Follicular growth and estradiol follow-up after subcutaneous xenografting of fresh and cryopreserved human ovarian tissue

Cited by 61 publications

References 55 publications

Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning

Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning

Quality and functionality of human ovarian tissue after cryopreservation using an original slow freezing procedure

Follicle growth and oocyte development after ovary transplantation into back muscle of immune-intact adult castrated male mice

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