Fontes de contaminação de Yersinia enterocolitica durante a produção de leite

Tavares, Amanda do Rosário; Souza, Agnes I. A. de; Dulac, C.F.; Moreira, Lauren Machado; Domı́nguez, Lucas; González, H.; Cereser, Natacha Deboni; Timm, Cláudio Dias

doi:10.1590/1678-4162-8423

Cited by 2 publications

(5 citation statements)

References 19 publications

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“…In a study carried out in Rio Grande do Sul, the prevalence of Y. enterocolitica in raw milk was reported as 9.37% by means of molecular analysis, a little higher than found in this study. Additionally, the pathogen was identified in the water used in the milking parlor and in the hands of manipulators, emphasizing the risk of contamination during milk production due to lack of good milking practices (TAVARES et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…The increase in raw milk consumption is justified by the taste, convenience, cost, and high nutritional value (KAYLEGIAN et al, 2008); however, milk is an ideal medium for the multiplication of a wide variety of pathogenic bacteria, and acts as an important substrate for transporting microorganisms from infected animals to humans. Despite being produced by healthy animals, contamination of milk can occur during milking, from contaminated water, milking equipment, and from the hands of milkers (TAVARES et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…The pathogen has already been isolated from raw milk and its derivatives on ice used for human consumption, pork and its derivatives, poultry meat, as well as fecal material from humans and sick animals and from the environment (VIDON; DELMAS, 1981;FALCÃO et al, 2002;DALLAL et al, 2004;PINTO et al, 2006;FALCÃO et al, 2006;ARCURI et al, 2008;HANIFIAN;KHANI, 2012;RUUSUNEN et al, 2013;DARWISH et al, 2015). In Brazil, Y. enterocolitica was isolated from several foods, mainly in the states of São Paulo, Rio de Janeiro, and Rio Grande do Sul TAVARES et al, 2017). In 2007, 8792 cases of yersiniosis were reported in humans in the European Union, making Y. enterocolitica the third most isolated zoonotic agent in human enteritis, behind only Salmonella and Campylobacter (DARWISH et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil

Bertolini

Dutra

Guiraldi

et al. 2019

SCA

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Milk and its derivatives are good substrates for the proliferation of pathogenic and quality-deteriorating microorganisms, demanding rigorous care with milking, processing, and storage. Among the various bacteria that can grow in raw refrigerated milk, Yersinia enterocolitica, is an invasive enteropathogen of humans. This bacterium can cause a number of intestinal and extraintestinal clinical symptoms, ranging from mild gastroenteritis to mesenteric lymphadenitis, similar to appendicitis. To evaluate the prevalence of pathogenic Yersinia enterocolitica in raw milk from bulk milk tanks located in the State of São Paulo, 102 bovine milk samples (one per dairy farm) were evaluated by microbiological analyses, followed by biochemical tests PCR and genetic sequencing. Microbiological testing did not isolate Y. enterocolitica. However, PCR analysis revealed six samples that were positive for Y. enterocolitica (5.9%), confirmed by genetic sequencing. Only the inv gene was detected, which is present in virulent and avirulent Y. enterocolitica strains. There was great difficulty in microbiological isolation due to the difficulty of competitiveness of Y. enterocolitica in a very rich microbiota of raw milk. Although virulence genes known to be present in potentially pathogenic strains of Y. enterocolitica have not been identified, the presence of this pathogen in milk from expansion tanks, identified through PCR and confirmed by genetic sequencing, suggests that Y. enterocolitica may be a risk to public health, especially if milk and its derivatives are consumed without heat treatment. ResumoO leite e seus derivados possuem substratos que favorecem o desenvolvimento de micro-organismos patogênicos e deteriorantes, devido a isso, cuidados rigorosos são exigindo durante a ordenha, processamento e seu armazenamento. Dentre os vários grupos de bactérias que podem contaminar o leite refrigerado cru está Yersinia enterocolitica. Esta bactéria pode causar a uma série de sintomas clínicos intestinais e extraintestinais, variando de gastroenterite leve a linfadenite mesentérica, semelhante à apendicite. Para avaliar a prevalência de Y. enterocolitica patogênica no leite cru de tanques de expansão localizados no estado de São Paulo, foram avaliadas 102 amostras de leite bovino, por análises microbiológicas, seguido de provas bioquímicas; a Reação em Cadeia de Polimerase (PCR) e sequenciamento genético. Não houve o isolamento de Y. enterocolitica pelas provas microbiológicas clássicas. No entanto, a análise de PCR, realizada diretamente do leite, revelou seis (6) amostras positivas para Y. enterocolitica (5,9%), confirmadas por sequenciamento genético. Somente o gene inv, foi detectado, que pode estar presente em cepas virulentas e avirulentas. Houve dificuldade de isolamento microbiológico devido à dificuldade de competitividade da Y. enterocolitica em uma microbiota muito rica do leite cru. Ainda que não tenham sido identificados os genes de virulência que sabidamente estão presentes em cepas de Y. enterocolitica potencialmente pat...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil

Bertolini

Dutra

Guiraldi

et al. 2019

SCA

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“…For the sampling of the pipe walls, swabs were rubbed over the inner surface of these pipes and along the entire extent of these tubular structures up to a 10 cm height. Samples from the insufflators were collected by rubbing the swabs in circular movements in the terminal segment of each of these devices (Tavares et al, 2017).…”

Section: Sample Collectionmentioning

confidence: 99%

“…For the sampling of raw milk, the collection was performed directly from the refrigeration tank after homogenization. With the aid of a sterilized spoon, 200 mL samples were collected and stored in sterile glass vials (Tavares et al, 2017).…”

Section: Archives Ofmentioning

confidence: 99%

Campylobacter sp. EM VACAS LEITEIRAS DO SUL DO BRASIL

Silveira

Kaefer

Porto

et al. 2021

AVS

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O presente estudo teve como objetivo avaliar a ocorrência de Campylobacter jejuni, Campylobacter coli e Salmonella enterica em vacas leiteiras e salas de ordenha de fazendas leiteiras no sul do Brasil. Foram coletadas amostras de 12 fazendas leiteiras localizadas no sul do Rio Grande do Sul. Durante as visitas às propriedades, foram coletadas amostras da água utilizada na sala de ordenha, dos insufladores das ordenhadeiras mecânicas, da superfície interna dos baldes de leite ou da parede da tubulação, das mãos dos manipuladores e do leite de conjunto foram coletadas para as análises microbiológicas. Também foram coletadas amostras de fezes de cinco vacas em produção, as quais também foram avaliadas quanto à presença de Campylobacter e Salmonella, totalizando 10 amostras em cada fazenda leiteira. Não houve o isolamento de Salmonella em nenhuma das amostras que foram analisadas. Campylobacter foi isolado de três amostras (3/60; 0,05%) de fezes de vacas de uma mesma propriedade, sendo um dos isolados identificado como C. jejuni, esse isolado apresentou o gene cdt que codifica para a toxina citoletal distensiva. Conclui-se com o estudo que Campylobacter sp. pode ser encontrado em bovinos leiteiros em processo de ordenha, podendo oferecer risco de transmissão desse micro-organismo pelo contato direto ou indireto com as fezes dos animais contaminados.

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Fontes de contaminação de Yersinia enterocolitica durante a produção de leite

Cited by 2 publications

References 19 publications

Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil

Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in São Paulo, Brazil

Campylobacter sp. EM VACAS LEITEIRAS DO SUL DO BRASIL

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