Fontibacillus phaseoli sp. nov. isolated from Phaseolus vulgaris nodules

Abstract

A bacterial strain, designated BAPVE7BT, was isolated from root nodules of Phaseolus vulgaris in Spain. Phylogenetic analysis based on its 16S rRNA gene sequence placed the isolate into the genus Fontibacillus with Fontibacillus panacisegetis KCTC 13564T its closest relative with 97.1 % identity. The isolate was observed to be a Gram-positive, motile and sporulating rod. The catalase test was negative and oxidase was weak. The strain was found to reduce nitrate to nitrite and to produce β-galactosidase but the production of gelatinase, caseinase, urease, arginine dehydrolase, ornithine or lysine decarboxylase was negative. Acetoin production and aesculin hydrolysis were found to be positive. Growth was observed to be supported by many carbohydrates and organic acids as carbon source. MK-7 was identified as the predominant menaquinone and the major fatty acid (43.7 %) as anteiso-C15:0, as occurs in the other species of the genus Fontibacillus. Strain BAPVE7BT displayed a complex lipid profile consisting of diphosphatidylglycerol, phosphatidylglycerol, four glycolipids, four phospholipids, two lipids, two aminolipids and an aminophospholipid. Mesodiaminopimelic acid was detected in the peptidoglycan. The G+C content was determined to be 45.6 mol% (Tm). Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain BAPVE7BT should be considered a new species of genus Fontibacillus, for which the name Fontibacillus phaseoli sp. nov. is proposed (type strain, LMG 27589T, CECT 8333T).

Fontibacillus pullulanilyticussp.nov. isolated from soil

“…The DNA G+C content of DSHK107 T was 42.1 mol%, a value consistent with that of members of the genus Fontibacillus .…”

“…The predominant isoprenoid quinone found in strain DSHK107 T , menaquinone‐7 (MK‐7), was the same as that found in recognized members of the genus Fontibacillus .…”

“…The fatty acid composition of strain DSHK107 T and related Fontibacillus species were given in Table . The major fatty acid of strain DSHK107 T was anteiso‐C 15:0 , which is also the major fatty acids in all members of the genus Fontibacillus . The ratio of fatty acids iso‐C 15:0 , iso‐C 16:00 , and C 16:00 was relatively high compared with the related strains of the genus Fontibacillus .…”

“…Members of this genus are heterotrophic, facultatively anaerobic and rod‐shaped, motile, and possess menaquinone‐7 (MK‐7) as the predominant respiratory quinone . At the time of writing, this genus comprised four species with validly published names (http://www.bacterio.cict.fr/) and members have been isolated from soil of a warm spring, soil, Phaseolus vulgaris nodules and potato root .…”

Paenibacillus segetis sp. nov., isolated from soil of a tropical rainforest

“…Phylogenetic analysis of the 16S rRNA gene sequence of strain DB13260 T showed a close relationship of the novel strain to members of the genus Paenibacillus. The highest 16S rRNA gene sequence similarity values were found with Paenibacillus anaericanus MH21 T (Horn et al, 2005), Paenibacillus selenii W126 T (Xiang et al, 2014) and Fontibacillus phaseoli BAPVE7B T (Flores-felix et al, 2014), with values of 98.3 %, 97.8 % and 97.3 %, respectively. Meanwhile, the other 16S rRNA gene sequence similarities were under 97.0 %.…”