2011
DOI: 10.1039/b907695f
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Food analysis and food authentication by peptide nucleic acid (PNA)-based technologies

Abstract: This tutorial review will address the issue of DNA determination in food by using Peptide Nucleic Acid (PNA) probes with different technological platforms, with a particular emphasis on the applications devoted to food authentication. After an introduction aimed at describing PNAs structure, binding properties and their use as genetic probes, the review will then focus specifically on the use of PNAs in the field of food analysis. In particular, the following issues will be considered: detection of genetically… Show more

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Cited by 60 publications
(18 citation statements)
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“…Biosensors have also been designed to detect microbial contaminants and various hormones in food(Xu and Ying, 2011) which provide us with the advantage of high degree of specificity and sensitivity, and the possibility of being used for inline processes monitoring during food manufacturing(Viswanathan et al, 2009). A similar new molecular approach includes the use of peptide nucleic acid (PNA)-based technologies for food analysis and food authentication(Sforza et al, 2011). Although molecular methodology for adulteration detection has stepped into the era of technique of adulterant detection and proves itself as best technique but still at industrial level its practical application has to go long way.…”
mentioning
confidence: 99%
“…Biosensors have also been designed to detect microbial contaminants and various hormones in food(Xu and Ying, 2011) which provide us with the advantage of high degree of specificity and sensitivity, and the possibility of being used for inline processes monitoring during food manufacturing(Viswanathan et al, 2009). A similar new molecular approach includes the use of peptide nucleic acid (PNA)-based technologies for food analysis and food authentication(Sforza et al, 2011). Although molecular methodology for adulteration detection has stepped into the era of technique of adulterant detection and proves itself as best technique but still at industrial level its practical application has to go long way.…”
mentioning
confidence: 99%
“…Detecting specific nucleic acids in food allows one to determine the presence of particular ingredients in complex samples or to detect characteristic features of selected food constituents. DNA analysis has been used to meet needs such as GMO detection, microbial pathogen determination, or detection of undeclared allergenic components (Sforza et al., ). Polymerase chain reaction (PCR) allows trace amounts of degraded nucleic acids to be detected and their sequence established.…”
Section: Food Authentication Techniquesmentioning
confidence: 99%
“…(iii) Other developments include the use of peptide nucleic acid (PNA)-based technologies for food analysis and food authentication [74] or the development of new immunoassay methodologies for analyzing veterinary drug residues in foods and food products [75] or to characterize plant food allergens [76].…”
Section: Food Analysis: Current State Of the Art Methodologies And mentioning
confidence: 99%