Food and feed components for gut health‐promoting adhesion of <i>E. coli</i> and <i>Salmonella enterica</i>

Becker, P.M.; Galletti, S.

doi:10.1002/jsfa.3324

Cited by 36 publications

(25 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The reason that palm kernel meal requires more CFU compared to other feed materials is presently unknown. However, Becker and Galletti observed that palm kernel meal effectively adhered to Salmonella, possibly because of high levels of mannans (9). Consequently, these results suggest that regardless of the analytical procedure employed, Salmonella contamination rates for palm kernel meal are likely underestimated.…”

Section: Discussionmentioning

confidence: 93%

Accuracy and Sensitivity of Commercial PCR-Based Methods for Detection of Salmonella enterica in Feed

Koyuncu

Andersson

Häggblom

2010

Appl Environ Microbiol

View full text Add to dashboard Cite

The present study compared the performance of commercial PCR-based Salmonella enterica detection methods (BAX System Q7, the iQ-Check Salmonella II kit, and the TaqMan Salmonella enterica detection kit) with culturebased methods (modified semisolid Rappaport-Vassiliadis [MSRV] and NMKL71) in spiked and naturally contaminated samples of feed mill scrapings (FMS), palm kernel meal (PKM), pelleted feed (PF), rape seed meal (RSM), soybean meal (SM), and wheat grain (WG). When results from the various feeds were compared, the number of Salmonella enterica CFU/25 g required to produce a positive were as follows: PKM > FMS ‫؍‬ WG > RSM ‫؍‬ SM ‫؍‬ PF. These data are similar to those developed in earlier studies with culture-based Salmonella detection methods. PCR-based methods were performed similarly to culture-based methods, with respect to sensitivity and specificity. However, many PCR positives could not be confirmed by Salmonella isolation and for that reason the evaluated methods were found to be suitable only when rapid results were paramount. Nevertheless, PCR-based methods cannot presently replace culture-based methods when typing information is required for tracing studies or epidemiological investigations. The observed difference in detection levels is a potential problem when prevalence data are compared as well as when feed ingredients are tested for conformance with microbiological criteria. This paper also presents a statistical model that describes the detection probability when different levels (CFU) of Salmonella contamination are present in feed materials.

show abstract

Section: Discussionmentioning

confidence: 93%

Accuracy and Sensitivity of Commercial PCR-Based Methods for Detection of Salmonella enterica in Feed

Koyuncu

Andersson

Häggblom

2010

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Ofek et al (2003a) describes that-apart from adhesion-receptor interaction-hydrophobic and other non-specific interactions might be involved in the adhesion process as well. Becker and Galletti (2008) investigated various food and feed components for alternative adhesion of intestinal E. coli and Salmonella. Yeast mannan oligosaccharides, pumpkin, sesame seed extract, palm kernel extract, and konjac gum were among the strongest binding matrices for enteropathogens.…”

Section: Mode(s) Of Action Anti-adhesion Approachmentioning

confidence: 99%

“…Thus, indication is given that unspecific, unknown binding properties are involved in this process. Moreover, taking the diversity of natural products due to biological variation for processing into account, the binding capacity of fibrous food and feed components for different bacteria seems rather unpredictable (Becker and Galletti 2008).…”

Section: Mode(s) Of Action Anti-adhesion Approachmentioning

confidence: 99%

Capability of yeast derivatives to adhere enteropathogenic bacteria and to modulate cells of the innate immune system

Ganner¹,

Schatzmayr²

2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Yeast derivatives including yeast cell wall components are promising alternatives to antibiotics with respect to the promotion of health and performance in livestock, based on their capacity to bind enteropathogenic bacteria and to beneficially modulate the immune system. However, these mode(s) of action both in vitro and in vivo are still not well understood. Furthermore, standardization and reproducibility of in vitro techniques (microbiology, cell culture assays) are critical features for the application of yeast derivatives as well as for the proof of effectiveness. Yeast cell wall products are suggested as anti-adhesive agents and are thus proposed to prevent attachment of certain intestinal bacteria by providing alternative adhesion sites to enterobacteria, which contain mannose-specific type I fimbriae such as Escherichia coli or Salmonella spp. and which is well documented. Various in vitro assay techniques have become of paramount importance for biotechnological research since they allow for determination and quantification of potential mode(s) of action. However, in vitro assays may be criticized by product end users as not accurately reflecting in vivo responses. Pro and cons of different assays and their bias will be discussed specifically regarding yeast cell wall components and adhesion of enteropathogenic bacteria. Immunomodulation is a therapeutic approach intervening in auto-regulating processes of the defense system. Yeast derivatives such as beta-glucans are proposed to interact with cells of the innate immune system by receptor recognition. Controversial data in literature and mode(s) of action are reviewed and discussed here.

show abstract

“…MOS would also have an additional important antimicrobial effect based on their capacity to bind to mannose‐specific lectin of gram‐negative pathogens that express type‐1 fimbriae (Borowsky et al . ), thus blocking the adhesion of these bacteria to the enterocytes and preventing the subsequent colonization (Becker and Galletti ). This binding capacity may, however, be very variable because MOS come from a wide variety of sources (oil palm seeds, coffee beans, yeast, carob seeds, etc.…”

Section: Introductionmentioning

confidence: 99%

Reduction of subclinical Salmonella infection in fattening pigs after dietary supplementation with a ß-galactomannan oligosaccharide

et al. 2014

View full text Add to dashboard Cite

Aims: To assess the efficacy of a b-galactomannan oligosaccharide (ß-GMOS) for the control of Salmonella infection in fattening pigs. Methods and Results: Three different doses (0Á5, 3 and 2 kg ß-GMOS per ton of feed) were used during the entire period of growing in three similar and independent field trials carried out in a small fattening unit (%100 pigs). Treatment was randomly assigned to half of the pens. Individual serum samples (20-25 per group) were collected at different times during the fattening period and a similar number of faecal samples during the fattening period and at slaughter. In addition, mesenteric lymph nodes were collected at slaughter. Herdcheck â Swine Salmonella ELISA was used for serological analyses, the ISO 6579:2002/Amd 1 : 2007 for bacteriology and the PFGE for molecular characterization of Salmonella strains. The addition of ≥2 kg t À1 of ß-GMOS to the pig diet during the entire fattening period was associated with a reduction in Salmonella prevalence, shedding and seroconversion. Conclusions: Feed supplementation with ß-GMOS may be a useful complementary tool for the control of salmonellosis in fattening pigs. Significance and Impact of the Study: ß-GMOS may be a complementary way of reducing Salmonella shedding and infection in fattening pigs.

show abstract

Food and feed components for gut health‐promoting adhesion of E. coli and Salmonella enterica

Cited by 36 publications

References 56 publications

Accuracy and Sensitivity of Commercial PCR-Based Methods for Detection of Salmonella enterica in Feed

Accuracy and Sensitivity of Commercial PCR-Based Methods for Detection of Salmonella enterica in Feed

Capability of yeast derivatives to adhere enteropathogenic bacteria and to modulate cells of the innate immune system

Reduction of subclinical Salmonella infection in fattening pigs after dietary supplementation with a ß-galactomannan oligosaccharide

Contact Info

Product

Resources

About