Food security, obesity, and meat-derived carcinogen exposure in US adults

Dolan, Luke; Smith, Kristen S.; Marlin, Michael; Bell, Leonard N.; Blythe, Eugene K.; Greene, Michael W.; Frugé, Andrew D.

doi:10.1016/j.fct.2021.112412

Cited by 7 publications

(7 citation statements)

References 17 publications

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“…Participant consent was inferred by commencement of the survey. Recruitment of participants took place from 26–29 June 2020 through the online portal Amazon Mechanical Turk to complete the survey instrument [ 12 , 13 ]. Requirements for survey participation were to be 18 years of age or older along with a requirement to reside within the United States and have a HIT Approval Rate >98%.…”

Section: Methodsmentioning

confidence: 99%

“…The survey instrument included several components which aimed to answer different research questions. Elements not included in this study were previously reported by Dolan et al and discrepancies in inclusion/exclusion criteria resulted in different final sample sizes [ 12 ]. Fourteen questions were modified from the revised Morgenstern PA questionnaire (PAQ-M) by Rubenstein et al in 2011 [ 14 ] to assess time spent in different activities during the previous week.…”

Section: Methodsmentioning

confidence: 99%

“…Height and weight were self-reported, and two questions were added and designed to assess diet quality by measuring the number of meals consumed per week by content and means of obtainment and/or preparation. Lastly, three questions served as attention check questions to screen for invalid responses [ 12 ]. Additional survey questions allowed respondents to self-assess their behaviors relative to the previous (2019) year.…”

Section: Methodsmentioning

confidence: 99%

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Early Pandemic Improvements in Diet Quality Are Associated with Increased Physical Activity and Weight Loss in US Adults

Gautreaux

Smith

Dolan

et al. 2022

IJERPH

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In March 2020, the COVID-19 pandemic led to restricted vocational (Voc-PA) and recreational physical activity (Rec-PA) outside of the home. We conducted a nation-wide survey in the United States (US) during the mitigation peak of the pandemic (June 2020) to assess health-related changes from the previous year. A diet quality (DQ) assessment tool weighted the relative healthfulness of eating occasions from foods prepared-at-home (Home) and away-from-home (Away). Previously-validated instruments assessed PA and demographic variables; height/weight were self-reported to calculate body mass index (BMI). T-tests explored longitudinal, between-sex, and obesity status differences in DQ, PA, and BMI; Pearson correlations explored associations. Of 1648 respondents, 814 valid responses (56.8% female, 81.7% white) were analyzed. Overall and Home DQ was higher for females than males in 2020 (p < 0.001 for both). Respondents increased DQ from 2019 to 2020, primarily from Away (p < 0.001 for both sexes). Total Rec-PA and Voc-PA was higher in males (p = 0.002, p < 0.001) than females in 2020; females reported higher other PA (p = 0.001). Change in BMI was inversely associated with change in both DQ and PA (p < 0.001 for both). In this sample of US adults, early adaptations to the COVID-19 pandemic included improved DQ and BMI. Whether these short-term improvements were maintained warrant further investigation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Early Pandemic Improvements in Diet Quality Are Associated with Increased Physical Activity and Weight Loss in US Adults

Gautreaux

Smith

Dolan

et al. 2022

IJERPH

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“…Acrolein is a highly reactive unsaturated aldehyde that exhibits cytotoxic and broad-spectrum antimicrobial activities (Stevens and Maier, 2008;Engels et al, 2016b;Zhang and Schwab, 2022). In addition, acrolein has recently been reported to be involved in the detoxification of xenobiotic heterocyclic amines (HCAs) (Zhang et al, 2019a;Zhang et al, 2019b), which can be produced during the cooking of meat due to the high-temperature pyrolysis of amino acids and are suggested to be involved in the initiation of colorectal carcinogenesis (CRC) (Bouvard et al, 2015;Dolan et al, 2021;Kang et al, 2022). The transformation of HCA yields acrolein conjugates (Beer et al, 2017) with reduced cytotoxicity and mutagenicity, and may be beneficial for the host by decreasing HCA exposure and thus reducing CRC risk (Zhang et al, 2019b).…”

Section: Introductionmentioning

confidence: 99%

Anaerobutyricum hallii promotes the functional depletion of a food carcinogen in diverse healthy fecal microbiota

Ramirez Garcia,

Greppi,

Constancias

et al. 2023

Front. Microbiomes

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IntroductionAnaerobutyricum hallii is a human gut commensal that transforms the heterocyclic amine 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP), a carcinogen from cooked meat. The transformation mechanism involves the microbial production of acrolein from glycerol, and its conjugation with PhIP, thus blocking its mutagenic potential. A potential cancer prevention strategy could therefore involve supplementing complex human microbial communities with metabolically competent bacteria such as A. hallii that can deplete PhIP. However, it has not been established how the proportion of A. hallii in diverse healthy human gut microbial communities relates to functional capacity for PhIP transformation and, moreover, how supplementing microbiomes with A. hallii affects this function.MethodsIn this study, shotgun metagenomics was used to study taxonomic profiling, the abundance of glycerol/diol dehydratase (gdh)-harboring taxa, the proportion of resident A. hallii, and the reconstruction of A. hallii population genomes in the fecal samples of 20 healthy young adult donors. Furthermore, the influence of supplementing 106 cells/mL of A. hallii DSM 3353 with diluted fecal microbiota was characterized.Results and discussionSix microbiota were assigned to Bacteroides, nine to Prevotella, and five to Ruminococcus by enterotype-associated clustering. The total number of gdh copies in the 20 fecal microbiota expressed per 1010 bacterial cells ranged between 1.32 × 108 and 1.15 × 109. Eighteen out of the 20 donors were dominated by A. hallii, representing between 33% and 94% of the total gdh relative abundance of the samples. The microbiota with low A. hallii abundance (i.e., with a relative abundance < 1%) transformed less PhIP than the microbiota with high A. hallii abundance (i.e., with a relative abundance > 1%). Furthermore, supplementing the low-A. hallii-abundant microbiota with glycerol significantly increased the PhIP transformation capacity after 6 h while reducing total short-chain fatty acid (SCFA) levels, which is most likely due to acrolein production. Although acetate decreased in all microbiota with glycerol and with the combination of glycerol and A. hallii, for most of the microbiomes, butyrate production increased over time. Thus, for a significant number of diverse healthy human fecal microbiomes, and especially when they have little of the taxa to start with, supplementing A. hallii increases PhIP transformation. These findings suggest the need to test in vivo whether supplementing microbiomes with A. hallii reduces PhIP exposure.

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“…In most cases, CBMs are mixed with flavoring agents (e.g., benzaldehyde, ethyl butyrate, and acetoin), adipocytes (fat cells), and myoblasts (muscle cells), and then textured . CBMs combined with the above-mentioned components can raise serum cholesterol (approximately 20%), elevate blood pressure (20–30%), and may lead to the development of several diseases, such as pulmonary hypertension (9–37%), hyperlipidemia, and colorectal cancers, which are related to high protein/fat diets and characterized by an increased body mass index. , To date, there are two main strategies for CBM production: direct manipulation of bovine muscle cells, which includes the isolation, expansion, and mass production of bovine muscle cells, or generation of muscle progenitors from induced pluripotent stem cells cultured using a bioreactor . The first method is considered safe for human consumption. − The nutritional value of cultured meat also depends greatly on the cryoprotectants used for storage.…”

Section: Introductionmentioning

confidence: 99%

Bioengineered Lab-Grown Meat-like Constructs through 3D Bioprinting of Antioxidative Protein Hydrolysates

Dutta

Ganguly

Jeong

et al. 2022

ACS Appl. Mater. Interfaces

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Lab-grown bovine meat analogues are emerging alternatives to animal sacrifices for cultured meat production. The most challenging aspect of the production process is the rapid proliferation of cells and establishment of the desired 3D structure for mass production. In this study, we developed a direct ink writing-based 3D-bioprinted meat culture platform composed of 6% (w/v) alginate and 4% (w/v) gelatin (Alg/Gel)-based hydrogel scaffolds supplemented with naturally derived protein hydrolysates (PHs; 10%) from highly nutritive plants (soybean, pigeon pea, and wheat), and some selected edible insects (beetles, crickets, and mealworms) on in vitro proliferation of bovine myosatellite cells (bMSCs) extracted from fresh meat samples. The developed bioink exhibited excellent shear-thinning behavior (n < 1) and mechanical stability during 3D bioprinting. Commercial proteases (Alcalase, Neutrase, and Flavourzyme) were used for protein hydrolysis. The resulting hydrolysates exhibited lower-molecular-weight bands (12–50 kDa) than those of crude isolates (55–160 kDa), as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The degree of hydrolysis was higher in the presence of Alcalase for both plant (34%) and insect (62%) PHs than other enzymes. The 3D-printed hydrogel scaffolds displayed excellent bioactivity and stability after 7 days of incubation. The developed prototype structure (pepperoni meat, 20 × 20 × 5 mm) provided a highly stable, nutritious, and mechanically strong structure that supported the rapid proliferation of myoblasts in a low-serum environment during the entire culture period. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay enhanced the free radical reduction of Alcalase- and Neutrase-treated PHs. Furthermore, the bioprinted bMSCs displayed early myogenesis (desmin and Pax7) in the presence of PHs, suggesting its role in bMSC differentiation. In conclusion, we developed a 3D bioprinted and bioactive meat culture platform using Alg/Gel/PHs as a printable and edible component for the mass production of cultured meat.

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Food security, obesity, and meat-derived carcinogen exposure in US adults

Cited by 7 publications

References 17 publications

Early Pandemic Improvements in Diet Quality Are Associated with Increased Physical Activity and Weight Loss in US Adults

Early Pandemic Improvements in Diet Quality Are Associated with Increased Physical Activity and Weight Loss in US Adults

Anaerobutyricum hallii promotes the functional depletion of a food carcinogen in diverse healthy fecal microbiota

Bioengineered Lab-Grown Meat-like Constructs through 3D Bioprinting of Antioxidative Protein Hydrolysates

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