Foodborne disease outbreaks caused by sucrose-nonfermenting and β-galactosidase-deficient variants of Vibrio cholerae

Wei, Shiao-Wun; Chern, Lih-Ling; Wu, Yen-Chi; Wang, Yulan; Lin, Chia-Min; Chiou, Chien-Shun

doi:10.1016/j.ijfoodmicro.2007.11.061

Cited by 13 publications

(10 citation statements)

References 22 publications

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“…In 2004–2006, the V. cholerae variants isolated from four outbreaks in Taiwan were misidentified as V. mimicus and V. alginolyticus using an API 20E identification kit. However, these strains were correctly identified by several molecular techniques, including PCR [30].…”

Section: Discussionmentioning

confidence: 98%

Multiplex Real-time Polymerase Chain Reaction Assays for Simultaneous Detection of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus

Park

Jeon

Kim

et al. 2013

Osong Public Health and Research Perspectives

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ObjectivesA multiplex real-time polymerase chain reaction (RT-PCR) method was developed for the identification of three Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus.MethodsSpecific primers and probes targeting the hlyA, tlh, and vvhA genes were selected and used for multiplex real-time PCR to confirm the identification of V. cholerae, V. parahaemolyticus, and V. vulnificus, respectively. This method was applied to screen Vibrio species from environmental samples and combining it with a culture-based method, its effectiveness was evaluated in comparison with culture-based methods alone.ResultsSpecific PCR fragments were obtained from isolates belonging to the target species, indicating a high specificity of this multiplex real-time PCR. No cross-reactivity with the assay was observed between the tested bacteria. The sensitivity of the multiplex real-time PCR was found to have a lower limit of 104 colony-forming units/reaction for all three Vibrio species. The combination strategy raised the isolation ratio of all three Vibrio species 1.26- to 2.75-fold.ConclusionThis assay provides a rapid, sensitive, and specific technique to detect these three Vibrio species in the environment.

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Section: Discussionmentioning

confidence: 98%

Multiplex Real-time Polymerase Chain Reaction Assays for Simultaneous Detection of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus

Park

Jeon

Kim

et al. 2013

Osong Public Health and Research Perspectives

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“…The origin of the Haitian cholera outbreak was associated with contaminated water (Chin et al 2011). In addition, food borne outbreaks caused by this bacterium have also been reported in several Asian countries (Wei et al 2008).…”

Section: Introductionmentioning

confidence: 93%

“…In addition, food borne outbreaks caused by this bacterium have also been reported in several Asian countries (Wei et al . ).…”

Section: Introductionmentioning

confidence: 97%

A predictive model ofVibrio choleraefor combined temperature and organic nutrient in aquatic environments

Shen

Liu

et al. 2012

J Appl Microbiol

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Aims: To develop a predictive model for Vibrio cholerae in sea water. Methods and Results: The growth curves of V. cholerae NE-9 at different temperatures (range from 10 to 30°C) and chemical oxygen demand (COD) concentration (range from 5 to 40 mg l À1 ) were determined. The modified logistic model and Baranyi model were chosen to regress the growth curves. A new method for modelling effects of temperature and COD on the specific growth rate (l) was successfully developed by a combination of modified square root-type equation and saturation growth rate model. The coefficient of determination (R 2 ), bias factor (Bf) and accuracy factor (Af) were taken to assess the performance of the established model. Logistic model produced a good fit to the observed data (R 2 = 0Á952). However, the Baranyi model provided biologically plausible parameter estimates. The overall predictions for V. cholerae NE-9 growth agreed well with observed plate counts, and the average R 2 , Bf and Af values were 0Á967, 1Á198 and 1Á201, respectively. Conclusion: The predicted model agreed well with observed data, and the result can be applied for the prediction of V. cholerae in actual environments. Significance and Impact of the Study: The results of this study provide the basis for the prediction of V. cholerae in sea water.

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“…In each case, hygiene was poor and there were opportunities for pathogen growth in food. Although we lack any information on other vibrios implicated in food worker outbreaks, such outbreaks may occur, especially because variants of V. cholerae may be confused with other Vibrio species, such as Vibrio mimicus and Vibrio alginolyticus (207).…”

Section: Infective Doses For Foodborne and Other Enteric Pathogensmentioning

confidence: 99%

Outbreaks Where Food Workers Have Been Implicated in the Spread of Foodborne Disease. Part 4. Infective Doses and Pathogen Carriage

Todd

Greig

Bartleson³

et al. 2008

Journal of Food Protection

120

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In this article, the fourth in a series reviewing the role of food workers in foodborne outbreaks, background information on the presence of enteric pathogens in the community, the numbers of organisms required to initiate an infection, and the length of carriage are presented. Although workers have been implicated in outbreaks, they were not always aware of their infections, either because they were in the prodromic phase before symptoms began or because they were asymptomatic carriers. Pathogens of fecal, nose or throat, and skin origin are most likely to be transmitted by the hands, highlighting the need for effective hand hygiene and other barriers to pathogen contamination, such as no bare hand contact with ready-to-eat food. The pathogens most likely to be transmitted by food workers are norovirus, hepatitis A virus, Salmonella, Shigella, and Staphylococcus aureus. However, other pathogens have been implicated in worker-associated outbreaks or have the potential to be implicated. In this study, the likelihood of pathogen involvement in foodborne outbreaks where infected workers have been implicated was examined, based on infectious dose, carriage rate in the community, duration of illness, and length of pathogen excretion. Infectious dose estimates are based on volunteer studies (mostly early experiments) or data from outbreaks. Although there is considerable uncertainty associated with these data, some pathogens appear to be able to infect at doses as low as 1 to 100 units, including viruses, parasites, and some bacteria. Lengthy postsymptomatic shedding periods and excretion by asymptomatic individuals of many enteric pathogens is an important issue for the hygienic management of food workers.

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Foodborne disease outbreaks caused by sucrose-nonfermenting and β-galactosidase-deficient variants of Vibrio cholerae

Cited by 13 publications

References 22 publications

Multiplex Real-time Polymerase Chain Reaction Assays for Simultaneous Detection of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus

Multiplex Real-time Polymerase Chain Reaction Assays for Simultaneous Detection of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus

A predictive model ofVibrio choleraefor combined temperature and organic nutrient in aquatic environments

Outbreaks Where Food Workers Have Been Implicated in the Spread of Foodborne Disease. Part 4. Infective Doses and Pathogen Carriage

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