Although cephalopods play a critical role in marine food webs both as predators and prey, there is a limited knowledge of several basic aspects of their ecology, including their habitat and trophic level, in the Southern Ocean. We examined the ecological role of several Southern Ocean cephalopod species by analyzing δ N values were more variable among species, ranging from 2.4 to 13.3 ‰, a difference of ~11 ‰ that represents approx. 3 trophic levels. Differences among islands in isotope ratios in the same cephalopod species (higher δ 15 N and lower δ 13 C values in South Georgia) were attributed to regional oceanographic processes. Antarctic cephalopods occupy niches similar to those found in some pelagic fish, seabirds and marine mammals. As cephalopods are key components in Southern Ocean food webs, these results greatly advance our understanding of the structure, energy and carbon flows in this polar ecosystem.