Förderung der zellproliferation unter der einwirkung von 2-chloräthylphosphorsäure bei explantaten von Begonia x richmondensis in steriler kultur

Ringe, Friedhelm

doi:10.1016/s0044-328x(72)80034-5

Cited by 4 publications

(2 citation statements)

References 40 publications

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“…The reference [22] reported that direct shoot formation on Begonia x richmondensis stem segments was inhibited by 2-20 mg/l ethephon and completely prevented by 200 mg/l. In date palm tissue culture it was reported that AgNO 3 increased embryogenic callus weight in the absence of cytokinin, similar effects were noted upon the numbers of embryos formed, although here the number was reduced by an increase in AgNO 3 concentration [23].…”

Section: Table (2): Effect Of Various Concentrations Of Silver Thiosulphate (Sts) On Shoots Multiplication Of Date Palm Cvs Barhi and Maksupporting

confidence: 78%

The Role of Silver Thiosulphate and Glutamine on Direct Organogenesis of Two Date Palm (Phoenix dactylifera L.) Cultivars

Bader¹,

Khierallah²

2009

JoBRC

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This study was conducted to examine the effect of silver thiosulphate (STS) and glutamine at various concentrations on direct organogenesis and shoots multiplication of date palm (Phoenix dactylifera L.) Barhi and Maktom cvs. Shoot tip quarters were cultured in vitro on modified MS medium supplemented with 2.0 mg/1 2ip, 1.0 mg/1 BA, 1.0 mg/1 NAA and 1.0 mg/1 NOA with various concentrations of STS (30, 60, 90 and 120 µM) and glutamine at (0.35, 0.70, 1.4 and 2.1 mM). The same concentrations were used in multiplication stage with medium supplemented with 4.0 mg/1 2ip, 2 mg/1 BA, 1.0 mg/1 NAA and 1.0 mg/1 NOA. Results indicated that the addition of STS at 90 µM and glutamine at 0.7 mM gave the best result for adventitious bud formation after 16 weeks (8.1 and 9.4 buds per explant for Barhi and Maktom respectively). Subculturing of these buds on agitated liquid MS medium with 90 µM STS and 1.4 mM glutamine gave the highest average of shoots number (12.5 and 14.7 shoots for Barhi and Maktom respectively). Maktom cultivar produced more buds and shoots than Barhi irrespective of the level of STS and glutamine. Shoots were rooted and successfully transferred to soil. These results confirm the importance of supplementing plant culture media of date palm cvs. Barhi and Maktom with both silver thiosulphate and glutamine in order to optimize and improve the clonal propagation protocol for these two cultivars.

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Section: Table (2): Effect Of Various Concentrations Of Silver Thiosulphate (Sts) On Shoots Multiplication Of Date Palm Cvs Barhi and Maksupporting

confidence: 78%

The Role of Silver Thiosulphate and Glutamine on Direct Organogenesis of Two Date Palm (Phoenix dactylifera L.) Cultivars

Bader¹,

Khierallah²

2009

JoBRC

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“…The present results in total also confirm George & Sherrington's (1984) review and conclusion that the concentrations of ethylene that develop in culture flasks (particularly those that are tightly closed) can be sufficient to modify organogenesis and morphogenesis and plantlet development. Adventitious shoot formation might be either inhibited or stimulated through ethylene of the culture vessel, depending on the concentration and the period of accumulation (Ringe 1972;Huxter et al 1981). The role of ethylene in multiplication through axillary budding is by no means clear.…”

Section: Discussionmentioning

confidence: 99%

The influence of ethylene on proliferation and growth of rose shoot cultures

Kevers

Boyer

Courduroux

et al. 1992

Plant Cell Tiss Organ Cult

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Ethylene accumulation in four different rose in vitro culture containers was evaluated. Multiplication rate was the highest, and axes most elongated, in the two containers where ethylene accumulation was limited. Pulse treatments of ethylene at various concentrations enhanced proliferation depending on concentration (5 ppm generally was the most favourable) and time of application, while reducing elongation of the shoots. An ethylene trap in the flask atmospheres of the cultures reduced rose shoot proliferation rate but increased elongation of the axes. Inhibitors of ethylene biosynthesis, aminoethoxyvinylglycine (AVG) and cobalt chloride (COC12), increased multiplication rate by providing a higher number of axes of a suitable size for subculture. The ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) had a beneficial effect on multiplication rate, although reducing longitudinal growth of the axes.

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Plant Growth Regulators III : Gibberellins, Ethylene, Abscisic Acid, their Analogues and Inhibitors; Miscellaneous Compounds.

George¹,

Hall

Klerk³

Plant Propagation by Tissue Culture

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Förderung der zellproliferation unter der einwirkung von 2-chloräthylphosphorsäure bei explantaten von Begonia x richmondensis in steriler kultur

Cited by 4 publications

References 40 publications

The Role of Silver Thiosulphate and Glutamine on Direct Organogenesis of Two Date Palm (Phoenix dactylifera L.) Cultivars

The Role of Silver Thiosulphate and Glutamine on Direct Organogenesis of Two Date Palm (Phoenix dactylifera L.) Cultivars

The influence of ethylene on proliferation and growth of rose shoot cultures

Plant Growth Regulators III : Gibberellins, Ethylene, Abscisic Acid, their Analogues and Inhibitors; Miscellaneous Compounds.

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