The influence of ethylene on proliferation and growth of rose shoot cultures

Kevers, Claire; Boyer, Nicole; Courduroux, J. C.; Gaspar, Thomas

doi:10.1007/bf00055514

Cited by 41 publications

(17 citation statements)

References 22 publications

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“…Moreover, in aerated flasks, the number of shoots, leaf expansion and the number of leaves increased. Kevers et al (1992) showed that the multiplication rate and elongation of roses were higher in container types where ethylene accumulation was limited, however pulse treatments with C 2 H 4 during the first weeks of culture could enhance proliferation. Accumulation of CO 2 and C 2 H 4 during the first 10-15 days of Pinus radiata cultures promoted morphogenesis, while excessive accumulation after the initiation of buds caused some degree of dedifferentiation (Kumar et al, 1987).…”

Section: Resultsmentioning

confidence: 97%

The effect of headspace renewal in a Temporary Immersion Bioreactor on plantain (Musa AAB) shoot proliferation and quality

Roels

Noceda

Escalona

et al. 2005

Plant Cell Tiss Organ Cult

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The positive effect of ventilation of the culture container on in vitro shoot proliferation and quality was already proven for different species. Hereafter we report on the evolution of the headspace during in vitro culture of plantain in a Temporary Immersion Bioreactor (TIB) on the one hand, and culture on semi-solid medium on the other hand. The CO 2 and C 2 H 4 concentration reached a maximum of 12% and 0.45 ll l )1 , respectively in the control treatment on semi-solid medium, compared to 5.7% CO 2 and 0.06 ll l )1 C 2 H 4 in TIB. The minimal O 2 -concentration on semi-solid medium was 15.1%, compared to 19.3% in TIB. The multiplication rate was best in TIB, 6.4 compared to 4.3 in semi-solid conditions, and this was also the case for shoot height (4.3 cm compared to 3.3 cm), and leaf number (2.6 compared to 1.6). Moreover shoots produced on semi-solid medium showed distorted leaves. A typical day-night pattern in CO 2 and O 2 concentration was observed in TIB, as well as on semi-solid medium; this is illustrative for the photosynthetic capacity of the plant material produced in both systems.

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Section: Resultsmentioning

confidence: 97%

The effect of headspace renewal in a Temporary Immersion Bioreactor on plantain (Musa AAB) shoot proliferation and quality

Roels

Noceda

Escalona

et al. 2005

Plant Cell Tiss Organ Cult

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“…In the given context, particular attention should be paid to the gaseous plant hormone ethylene, since Panizza et al [21], working with the in vitro culture of lavandin, have reported on the essential role of ethylene in cytokinin-inducible shoot proliferation. Although the exact levels of ethylene gas inside the culture tubes were not measured during the present study, the fact that additional sealing of culture vessels lead to increased amounts of accumulated ethylene is thoroughly established by other plant tissue culturists [14]. Thus, increased shoot proliferation under the conditions of reduced ventilation might be related to the reported ability of ethylene to cause defects in apical dominance through the decrease of auxin to cytokinin ratio [12].…”

Section: Methodsmentioning

confidence: 83%

Multiplication and growth of hybrid poplar(Populus alba × P. tremula)shoots on a hormone-free medium

Žiauka

Kuusienė

2014

Acta Biologica Hungarica

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the present study explored an alternative approach for poplar micropropagation, based on the restriction of gas exchange between inside and outside environments of culture vessel, rather than on the application of exogenous hormones. Apical and nodal stem segments (explants) excised from in vitro-developed shoots of hybrid white poplar (Populus alba L. × P. tremula L.) were incubated in either sealed (with Parafilm) or unsealed capped glass culture tubes (150 × 20 mm) on a hormone-free Woody Plant Medium. Shoot proliferation on apical explants was observed in sealed culture tubes but not in the unsealed ones; the difference between these two samples in respect of shoot number increased in the course of time and became threefold after three months of culture, with 3.2 ± 0.4 (mean ± SE) shoots per explant in the sealed tubes versus 1.1 ± 0.1 in the unsealed ones (for comparison, the mean shoot numbers on nodal explants were 2.4 ± 0.3 and 3.4 ± 0.4 in the unsealed and sealed culture tubes, respectively). Moreover, the shoots taken from the sealed culture tubes could be distinguished by superior shoot length, if compared to the shoots from the unsealed tubes, during the subsequent culture stage under uniform conditions.

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“…This indicated that the trace amount of ethylene (presumably below 0.02 ppm) promoted EF. Kevers et al (1992) pointed out that ethylene inhibits shoot growth. Pulse treatment of C 2 H 4 enhanced proliferation depending on the concentration and time of application.…”

Section: Effects Of Individual Gas Componentsmentioning

confidence: 98%

Induction of somatic embryos of celery by control of gaseous compositions and other physical conditions

2006

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The celery (Tall Utah, Apium graveolens var. dulce) was adopted as a model system to investigate effective methods for enhancing embryogenesis. The focus was placed on attaining the optimum gaseous composition and the mixing intensity and viscosity of culture systems. The mixing intensity was investigated by using creased flasks and evaluated by shear force index (SFI). By using different closures with different ventilation coefficients, Kw, to adjust the gaseous composition in the flasks, it was found that embryo frequency (EF) increased with high P O 2 or low ethylene concentration in the headspace of the culture flasks. Cultivating the embryogenic cells under controlled ethylene concentrations depressed EF at ethylene concentrations higher than 0.08 ppm. Moreover, 0.1 lM of CoCl 2 remarkably repressed the ethylene formation in the culture medium and enhanced EF. P CO 2 and P O 2 around 3% and 30%, respectively, favored EF. For non-creased flasks, addition of CMC (carboxymethyl cellulose) decreased the EF and DO level of the medium, due to lowered mass transfer rate attributed to high viscosity. For creased flasks, supplementation of 0.4 g/ 100 ml CMC reduced the ill-effects of high shear stress and maximized EF (7.22%). Our results are potentially applicable to other species and largescale embryo production systems.

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The influence of ethylene on proliferation and growth of rose shoot cultures

Cited by 41 publications

References 22 publications

The effect of headspace renewal in a Temporary Immersion Bioreactor on plantain (Musa AAB) shoot proliferation and quality

The effect of headspace renewal in a Temporary Immersion Bioreactor on plantain (Musa AAB) shoot proliferation and quality

Multiplication and growth of hybrid poplar(Populus alba × P. tremula)shoots on a hormone-free medium

Induction of somatic embryos of celery by control of gaseous compositions and other physical conditions

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