The effect of headspace renewal in a Temporary Immersion Bioreactor on plantain (Musa AAB) shoot proliferation and quality

Abstract

The positive effect of ventilation of the culture container on in vitro shoot proliferation and quality was already proven for different species. Hereafter we report on the evolution of the headspace during in vitro culture of plantain in a Temporary Immersion Bioreactor (TIB) on the one hand, and culture on semi-solid medium on the other hand. The CO 2 and C 2 H 4 concentration reached a maximum of 12% and 0.45 ll l )1 , respectively in the control treatment on semi-solid medium, compared to 5.7% CO 2 and 0.06 ll l )1 C 2 H 4 in TIB. The minimal O 2 -concentration on semi-solid medium was 15.1%, compared to 19.3% in TIB. The multiplication rate was best in TIB, 6.4 compared to 4.3 in semi-solid conditions, and this was also the case for shoot height (4.3 cm compared to 3.3 cm), and leaf number (2.6 compared to 1.6). Moreover shoots produced on semi-solid medium showed distorted leaves. A typical day-night pattern in CO 2 and O 2 concentration was observed in TIB, as well as on semi-solid medium; this is illustrative for the photosynthetic capacity of the plant material produced in both systems.

In vitro leaf anatomy, ex vitro photosynthetic behaviors and growth of Calathea orbifolia (Linden) Kennedy plants obtained from semi-solid medium and temporary immersion systems

“…1c, d; Table 1). This was probably due to the increasing in vitro ventilation by TIS system Roels et al 2006). More wax and lower stomatal frequency were also found on carnation leaves in ventilated conditions with a higher number of air exchanges (Majada et al 1998(Majada et al , 2001.…”

“…3), and had subsequent higher leaf area, fresh and dry weights after growing in a shaded greenhouse (Table 2). TIS or temporary immersion bioreactor provides a better handling of the in vitro gas environments and the nutrition than semi-solid medium (Escalona et al 2003;González-olmedo et al 2005;Roels et al 2006). Plantlets had more wax and lower stomatal frequency when cultured in containers with a high number of air exchanges (Cui et al 2000;Majada et al 2001;Lucchesini et al 2006).…”

Improved growth and quality of Siraitia grosvenorii plantlets using a temporary immersion system

“…Similarly, in the liquid medium, the base of shoots was totally immersed in the liquid medium during the whole culture period, and so the concentration of oxygen around the root system was also limited. However, renewal of the head space in the TIS with every immersion led to higher oxygen concentration (Roels et al 2006), which probably contributed to the well-developed root formation of S. grosvenorii plantlets.…”

Morphological and physiological responses of proliferating shoots of teak to temporary immersion and BA treatments

“…Different procedures have been developed to avoid these problems (Ziv, 2005). Among them, the use of temporary immersion systems (TIS) to improve in vitro growth and plant quality in different species (see review Etienne and Berthouly, 2002;Quiala et al 2006;Roels et al 2006;Aragón et al 2009, Pérez-Alonso et al, 2009Yan et al, 2010, Sankar-Thomas andLieberei, 2011). However, few forestry species, Eucalyptus sp (Castro and González, 2002;McAlister et al 2005), Pinus radiata D. Don (AitkenChristie and Jones, 1987) and Crescentia cujete L. (Murch et al 2004) have been successfully propagated by TIS.…”