Foregut Mesenchyme Contributes Cells to Islets during Pancreatic Development in a 3-Dimensional Avian Model

Lear, Pamela; Jayanthi, Naga Venkatesh; Teague, Warwick J.; Johnson, Paul

doi:10.4161/org.1.2.1254

Cited by 6 publications

(10 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These data support our previous findings that foregut mesenchymal tissue can also differentiate into islet tissue [3]. The ratio of splenic mesenchyme to chick pancreatic epithelia does not appear to influence this percentage, whereas doubling the amount of each tissue proportionately increases the percentage of recombinants in which this is observed (42% vs 80% recombinants).…”

Section: Discussionsupporting

confidence: 90%

“…The embryonic epithelium of each pancreatic bud was microdissected from its surrounding mesenchymal cortex using chemical digestion with collagenase, as described previously [3,4]. Chimeric chick-quail embryonic recombinants were constructed by recombining the embryonic quail spleens with the chick pancreatic epithelia.…”

Section: Chick-quail Chimerasmentioning

confidence: 99%

“…However, we have previously shown that foregut mesenchyme is also capable of differentiating into islets in an avian embryonic model [3]. We have also shown that it can differentiate into pancreatic acini and alpha cells [4,5], with a window of competency during development in which this occurs [6].…”

mentioning

confidence: 96%

See 2 more Smart Citations

The spleen—a potential source of new islets for transplantation?

Robertson

Rowan-Hull

Johnson

2008

Journal of Pediatric Surgery

Self Cite

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 90%

Section: Chick-quail Chimerasmentioning

confidence: 99%

See 1 more Smart Citation

The spleen—a potential source of new islets for transplantation?

Robertson

Rowan-Hull

Johnson

2008

Journal of Pediatric Surgery

Self Cite

View full text Add to dashboard Cite

“…The embryonic epithelium of each pancreatic bud or stomach rudiment was microdissected from its surrounding mesenchymal cortex using chemical digestion collagenase as described previously [4,5]. Representative epithelia and mesenchyme were removed at time zero and fixed for 15 minutes using 4% buffered formol saline before analysis to control for contamination of the mesenchymal source by residual epithelial cells.…”

Section: Chick-quail Chimerasmentioning

confidence: 99%

“…However, we have recently demonstrated that foregut mesenchyme can also contribute cells to both acini [4] and islets during embryonic development, that is, islet mesenchyme-to-epithelial transition (iMET) [5,6]. Foregut mesenchyme may, therefore, be a novel source of islet stem cells and warrants further investigation.…”

mentioning

confidence: 99%

The competency of foregut mesenchyme in islet mesenchyme-to-epithelial transition during embryonic development

Teague

Rowan-Hull

Jayanthi

et al. 2006

Journal of Pediatric Surgery

Self Cite

View full text Add to dashboard Cite

Foregut mesenchyme contributes cells to pancreatic acini during embryonic development in a chick?quail chimera model

et al. 2004

View full text Add to dashboard Cite

To understand causes of developmental abnormalities of the pancreas, it is essential to understand its normal embryonic development. Current understanding of the development of pancreatic exocrine tissue is that it develops solely from embryonic epithelium, while the role of the surrounding mesenchyme is to signal to this epithelium and form connective tissue. Recent work in our laboratory has shown that pancreatic bud mesenchyme can contribute cells to islets during embryonic development. However, no published studies have investigated in detail whether mesenchyme contributes cells to the exocrine structures of the pancreas. The aim of this study was to investigate whether cells from foregut mesenchyme can contribute to pancreatic acini during embryonic development. Chick-quail chimera recombinant organs were constructed using pancreatic epithelium and mesenchyme from either the pancreas (n=12) or stomach (n=25). These were cultured for 7 days in 3-D collagen gels. The resulting specimens were analysed using morphological criteria and fluorescent immunocytochemistry against pancreatic amylase, insulin, and the quail-specific nucleolar antigen QCPN. Two independent observers determined the origins of acini as either solely epithelial, solely mesenchymal, or of mixed origin. Results are expressed as percentages of total acini identified in each group. Statistical analysis was performed using chi(2) tests (P<0.01 was considered statistically significant). Recombinations of pancreatic epithelium and pancreatic mesenchyme yielded 11 acini, of which 45% were derived from epithelium only, 45% from mesenchyme only, and 10% of mixed origin. Recombinations of pancreatic epithelium and stomach mesenchyme yielded 78 acini, of which 40% were derived from epithelium only, 32% from mesenchyme only, and 28% of mixed origin. When acini with any mesenchymal cellular contribution were considered as a group, there was no significant difference between stomach and pancreatic mesenchymal contribution (P=0.72). This is the first study to demonstrate the cellular contribution of mesenchyme to pancreatic exocrine structures. Our data show that mesenchyme contributes cells to pancreatic acini during development in this model and that mesenchyme derived from stomach and pancreatic sources are both able to form acini.

show abstract

Foregut Mesenchyme Contributes Cells to Islets during Pancreatic Development in a 3-Dimensional Avian Model

Cited by 6 publications

References 45 publications

The spleen—a potential source of new islets for transplantation?

The spleen—a potential source of new islets for transplantation?

The competency of foregut mesenchyme in islet mesenchyme-to-epithelial transition during embryonic development

Foregut mesenchyme contributes cells to pancreatic acini during embryonic development in a chick?quail chimera model

Contact Info

Product

Resources

About