Forensic DNA analysis for animal protection and biodiversity conservation: A review

Iyengar, Arati

doi:10.1016/j.jnc.2013.12.001

Cited by 70 publications

(60 citation statements)

References 105 publications

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“…Forensic approaches have been adopted to identify wildlife animal species in need of conservation and to prevent their fraudulent misidentification, especially from samples of animal residues and the products made from processing their parts (Iyengar 2014;Tobe & Linacre 2010;Hsieh et al 2011;Ogden et al 2009). Advances in molecular science have launched a new era in the taxonomy of species identification, even with a bit of biological sample which, sometimes, is not possible to identify using conventional morphological methods.…”

Section: Introductionmentioning

confidence: 99%

DNA barcoding of the Indian blackbuck (Antilope cervicapra) and their correlation with other closely related species

Kumar

Sharma

2017

Egypt J Forensic Sci

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Background: Sequence divergence in mitochondrial cytochrome c oxidase subunit I genes (COI) has been used as an efficient forensic tool in solving wildlife-related problems and also be used in molecular taxonomy for species identification. Methods: This study presents the DNA barcode sequences of the Blackbuck (Antilope cervicapra) from Haryana, India. A dataset of 43 partial COI sequences of 13 species belonging to 4 genus of Antilopinae sub-family were used for molecular analysis and to construct the phylogenetic tree to elucidate the species diversity among sub-family Anitlopinae. The data comprised of generated sequences of the Blackbuck (n = 22) and with additional COI sequences of the related species (n = 21) showing maximum homology with Blackbuck COI sequence were downloaded from NCBI-GenBank for wide coverage of inter and intra-specific nucleotide diversity. Results: Phylogenetic analysis demonstrated that the studied COI region provided accurate species clustering showing their importance in wildlife species identification. The intra-specific sequence divergence of all the studied Antilopinae species was observed 0.47% (<1%). Specifically in the studied genus-Antilope, it was observed 0.3%. Whereas the interspecific divergence was in the range of 0.3-12.6% among 13 species from 4 genus. The highest interspecific divergence was observed among Antilope cervicapra and Gazella erlangeri (12.6%). Conclusion: The developed species-specific barcoding (COI) sequence of Indian Blackbuck from Haryana, India, demonstrated their high potential to identify Blackbuck from their sympatric species in wildlife-related crimes and in conservation activities. Published Barcode sequences in the both database serve as a wildlife forensic tool to solve Blackbuck related crimes. This study supports the credibility of DNA Barcoding in forensic investigation and wildlife conservation and also emphasized the need for species barcode database from each country to assist in solving wildlife related offenses.

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Section: Introductionmentioning

confidence: 99%

DNA barcoding of the Indian blackbuck (Antilope cervicapra) and their correlation with other closely related species

Kumar

Sharma

2017

Egypt J Forensic Sci

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“…El análisis de ADN forense permitió identificar la caza furtiva, el comercio ilegal y el contrabando ilegal de especies amenazadas, entre otros delitos (Iyengar 2014). En lo que respecta a mamíferos neotropicales, se usan distintos marcadores moleculares para identificar carne de guanaco (Lama glama), venado de las pampas (Ozotoceros bezoarticus) y carpincho (H. hydrochaeris) producto de la caza furtiva (Marín et al 2009;Sanches et al 2012).…”

Section: Nardelli and Ji Túnezunclassified

Aportes de la genética de la conservación al estudio de los mamíferos neotropicales: revisión y análisis crítico

Nardelli

Túnez

2017

Ecol. Austral

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R��.Si bien todos los seres vivos modifican su entorno, nuestra especie ha adquirido la capacidad de hacerlo a una escala espacio-temporal superlativa. Como resultado de la industrialización y del uso de nuevas tecnologías, el impacto antrópico ha ido en aumento en los últimos siglos, lo que causó la disminución de los tamaños poblacionales o la extinción de numerosas poblaciones silvestres. En este sentido, desde el campo de la genética de la conservación, en las últimas décadas se realizaron diversos esfuerzos para aportar conocimientos nuevos que contribuyan a la conservación de poblaciones, especies y hábitats. Las propiedades de los distintos marcadores moleculares han permitido estudiar la variabilidad genética de las especies y cómo esa variabilidad se estructura en las poblaciones y define las llamadas Unidades de Manejo, que son unidades genéticas cuyo nombre deriva de actividades extractivistas. En este artículo revisamos y analizamos críticamente los esfuerzos científicos en el área de la genética de la conservación realizados entre 2006 y 2015 en pos de la conservación de los mamíferos del Neotrópico. También criticamos el concepto de Unidad de Manejo y analizamos las perspectivas a futuro en el campo de la genética de la conservación de los mamíferos neotropicales.[Palabras clave: biología de la conservación, fauna neotropical, especies amenazadas, marcadores moleculares] A��. Contributions of conservation genetics to the study of neotropical mammals: review and critical analysis.Although all living beings modify their environment, our species has acquired the ability to do so on a superlative space-time scale. As a result of industrialization and the use of new technologies, the anthropic impact has been increasing in the last centuries, causing the decrease in population sizes or the extinction of numerous wild populations. In this sense, from the field of conservation genetics, diverse efforts have been made in the last decades in order to provide new knowledge that contributes to the conservation of populations, species and habitats. The properties of the different molecular markers have allowed studying the genetic variability of the species and how it is structured in the populations, defining the so-called Management Units, genetic units whose name derives from extractivist activities. In this article we perform a review and critical analysis of the scientific efforts in the area of conservation genetics carried out between the years 2006 and 2015 for the conservation of mammals of the Neotropics, criticize the concept of Management Unit and analyze future perspectives in the field of conservation genetics of neotropical mammals.

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“…Commonly used animal biological samples for morphological and DNA analyses are: muscles, hair, skin, feces, urine, horns and internal organs [7]. Materials such as fur, feathers, wool or traces of animals (imprints, saliva, and blood) can also be used as evidence material [8,9].…”

Section: Introductionmentioning

confidence: 99%

Improved DNA-Based Identification of Cervidae Species in Forensic Investigations

Davitkov

Glavinić

Nešić³

et al. 2017

Acta Veterinaria

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The main reasons for wildlife forensic research are animal poaching, illegal trade, and falsifi ed game meat products. Small trace amounts, old and degraded materials present the most common samples in revealing criminal activities in this fi eld. This is the reason why it is crucial to use adequate and reliable methods and samples to identify animal species killed outside the hunting season or species protected by law. In this study, different endpoint PCR and real-time PCR protocols were compared in the identifi cation of three Cervidae species (Capreolus capreolus, Cervus elaphus, Dama dama) from old and damaged material found in an enclosed area where the animals were kept. From a total of 129 samples, end point PCR provided results for 119 samples, while real-time PCR was successful in all cases. Also, we created and tested a protocol for simultaneous analyses of different types of samples, which is of great importance as when the amplifi cation is carried out simultaneously it is more cost effi cient and speeds up the process.

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Forensic DNA analysis for animal protection and biodiversity conservation: A review

Cited by 70 publications

References 105 publications

DNA barcoding of the Indian blackbuck (Antilope cervicapra) and their correlation with other closely related species

DNA barcoding of the Indian blackbuck (Antilope cervicapra) and their correlation with other closely related species

Aportes de la genética de la conservación al estudio de los mamíferos neotropicales: revisión y análisis crítico

Improved DNA-Based Identification of Cervidae Species in Forensic Investigations

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