Forensic genetic analysis of bone remain samples

Siriboonpiputtana, Teerapong; Rinthachai, T.; Shotivaranon, J.; Peonim, Vichan; Rerkamnuaychoke, Budsaba

doi:10.1016/j.forsciint.2017.12.045

Cited by 27 publications

(11 citation statements)

References 17 publications

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“…Costal cartilage is a neglected and underestimated material in forensic science and has not been elucidated in forensic medical guidelines. Recently, Siriboonpiputtana et al have demonstrated its usefulness as an alternative source for DNA typing in personal identification and shown that using costal cartilage enables faster and cost-effective DNA isolation than using hard tissues [23]. Our statistical analysis indicates a strong positive correlation between alcohol levels in UCC (unground costal cartilage) and GCC (ground costal cartilage) (r = 0.744, p < 0.001).…”

Section: Discussionmentioning

confidence: 54%

“…In forensic science, the costal cartilage is used to predict age by using computed tomography [20], measuring the racemization of aspartic acid [21] or estimating the ossification rate [22]. Recently, Siriboonpiputtana et al have demonstrated its usefulness as an alternative source for DNA typing in personal identification, enabling faster and cost-effective DNA isolation compared to hard tissues [23]. Nonetheless, the costal cartilage has not been included in forensic toxicology guidelines for ethanol level analysis [24], even though the penetration of ethanol into cartilage occurs, as the authors suspect, through the perichondrium and intercostal veins.…”

Section: Introductionmentioning

confidence: 99%

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A Comparative Study of Ethanol Concentration in Costal Cartilage in Relation to Blood and Urine

Tomsia

Nowicka²,

Skowronek³

et al. 2020

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Blood is not always available in forensic autopsies, therefore, the search for alternative sampling materials is needed. This study aimed at examining if ethanol can be detected in costal cartilage and to investigate if different forms of costal cartilage can give accurate information about ethanol concentration in the blood or urine of human cadavers (n = 50). Ethanol concentration in samples of unground costal cartilage (UCC), ground costal cartilage (GCC), femoral venous blood, and urine was analyzed using a gas chromatography-flame ionization detector (GC-FID). Due to Polish law, we used two different cut-off points: the blood alcohol concentration >0.2 mg/mL defined as the ‘after use’ condition, and the blood alcohol concentration >0.5 mg/mL defined as the ‘state of insobriety’. Based on the constructed receiver operating characteristics (ROC) curves, the optimal cut-off point for ethanol content as the ‘after use’ condition was 0.273 mg/g for the UCC method and 0.069 mg/g for the GCC method. Analysis of the Areas under a ROC Curve (AUC) showed that both methods present excellent diagnostic accuracy (AUCUCC = 0.903; AUCGCC = 0.984). We demonstrated that it is possible to detect ethanol in the costal cartilage and showed that ethanol concentrations are determined in GCC samples with greater accuracy.

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Section: Discussionmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Ethanol Concentration in Costal Cartilage in Relation to Blood and Urine

Tomsia

Nowicka²,

Skowronek³

et al. 2020

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“…New research has attempted to use costal cartilage to predict post-mortem interval basing on the extracellular matrix macromolecules degradation, but it seems to require further investigation [39]. More successfully, Siriboonpiputtana et al have demonstrated that costal cartilage can serve as an alternative source for DNA typing in personal identification, and it enables faster and more cost-effective DNA isolation than hard tissues [40].…”

Section: Discussionmentioning

confidence: 99%

Concentrations of volatile substances in costal cartilage in relation to blood and urine – preliminary studies

Tomsia¹,

Nowicka²,

Skowronek³

et al. 2021

amsik

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Aim:The study aimed to examine whether volatile substances (ethanol, isopropanol, and acetone) can be detected in costal cartilage and also if concentrations of detected substances reliably reflect their concentrations in the peripheral blood -the standard forensic material for toxicological analyses. Such knowledge can be useful in cases when a cadaver's blood is unavailable or contaminated. Material and methods: Ethanol, isopropanol, and acetone concentrations were determined in samples of unground costal cartilage (UCC), ground costal cartilage (GCC), femoral venous blood, and urine. The samples were analysed by gas chromatography (GC) with a flame ionization detector using headspace analysis. Results: Volatile substances were detected in 12 out of 100 analysed samples. There was a strong positive correlation between ethanol concentration in the blood and urine (r = 0.899, p < 0.001), UCC (r = 0.809, p < 0.01), and GCC (r = 0.749, p < 0.01). A similar strong correlation was found for isopropanol concentration in the blood and urine (r = 0.979, p < 0.001), UCC (r = 0.866, p < 0.001), and GCC (r = 0.942, p < 0.001). Acetone concentration in the blood strongly correlated only with its concentration in urine (r = 0.960, p < 0.001). Conclusions: We demonstrated for the first time the possibility of detecting volatile substances: ethanol, isopropanol and acetone in a human costal cartilage. Also, the study showed that higher volatiles concentrations were better determined in ground samples.

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“…In some cases, fragmented bones and teeth provide poor quality and low quantity genetic materials [31]. There have been advances in DNA extraction and purification, with the goal of improving DNA recovery and decreasing damage and inhibitors [32][33][34]. Several studies compared different kinds of more frequently used extraction methods [35][36][37] and found that total demineralization has the highest efficiency when performing extraction from highly degraded human remains.…”

Section: Molecular Analysismentioning

confidence: 99%

Fragment analysis in forensic anthropology

Ubelaker

2020

Forensic Sciences Research

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Anthropological analysis of fragmentary evidence can be challenging but diverse methods allow substantial information to be gleaned. Scanning electron microscopy/energy dispersive X-ray spectroscopy enables determination if bone and/or tooth tissue is present. Protein radioimmunoassay or DNA analysis can establish the species present. Histological analysis can assist in species determination and reveal information about thermal changes. Radiocarbon analysis with special reference to the modern bomb-curve can clarify the postmortem interval. Anthropologists should also be aware that DNA analysis not only can enable positive identification but assist in the evaluation of sex and age at death.

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Forensic genetic analysis of bone remain samples

Cited by 27 publications

References 17 publications

A Comparative Study of Ethanol Concentration in Costal Cartilage in Relation to Blood and Urine

A Comparative Study of Ethanol Concentration in Costal Cartilage in Relation to Blood and Urine

Concentrations of volatile substances in costal cartilage in relation to blood and urine – preliminary studies

Fragment analysis in forensic anthropology

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