Fork it over: the cohesion establishment factor Ctf7p and DNA replication

Skibbens, Robert V.; Maradeo, Marie E.; Eastman, Laura

doi:10.1242/jcs.011999

Cited by 33 publications

(38 citation statements)

References 52 publications

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“…In addition to the SCC2/SCC4 cohesin loading complex, active in late G1, cohesion establishing factors interact with components of the replication machinery such as the proliferating cell nuclear antigen, the replication factor C (RFC; reviewed in Guacci 2007;Skibbens et al 2007), and the origin recognition complex (reviewed in Diaz-Martinez et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Cohesin gene defects may impair sister chromatid alignment and genome stability in Arabidopsis thaliana

et al. 2009

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In contrast to yeast, plant interphase nuclei often display incomplete alignment (cohesion) along sister chromatid arms. Sister chromatid cohesion mediated by the multi-subunit cohesin complex is essential for correct chromosome segregation during nuclear divisions and for DNA recombination repair. The cohesin complex consists of the conserved proteins SMC1, SMC3, SCC3, and an alpha-kleisin subunit. Viable homozygous mutants could be selected for the Arabidopsis thaliana alpha-kleisins SYN1, SYN2, and SYN4, which can partially compensate each other. For the kleisin SYN3 and for the single-copy genes SMC1, SMC3, and SCC3, only heterozygous mutants were obtained that displayed between 77% and 97% of the wild-type transcript level. Compared to wild-type nuclei, sister chromatid alignment was significantly decreased along arms in 4C nuclei of the homozygous syn1 and syn4 and even of the heterozygous smc1, smc3, scc3, and syn3 mutants. Knocking out SYN1 and SYN4 additionally impaired sister centromere cohesion. Homozygous mutants of SWITCH1 (required for meiotic sister chromatid alignment) displayed sterility and decreased sister arm alignment. For the cohesin loading complex subunit SCC2, only heterozygous mutants affecting sister centromere alignment were obtained. Defects of the alpha-kleisin SYN4, which impair sister chromatid alignment in 4C differentiated nuclei, do apparently not disturb alignment during prometaphase nor cause aneuploidy in meristematic cells. The syn2, 3, 4 scc3 and swi1 mutants display a high frequency of anaphases with bridges (~10% to >20% compared to 2.6% in wild type). Our results suggest that (a) already a slight reduction of the average transcript level in heterozygous cohesin mutants may cause perturbation of cohesion, at least in some leaf cells at distinct loci; (b) the decreased sister chromatid alignment in cohesin mutants can obviously not fully be compensated by other cohesion mechanisms such as DNA concatenation; (c) some cohesin genes, in addition to cohesion, might have further essential functions (e.g., for genome stability, apparently by facilitating correct recombination repair of double-strand breaks).

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Section: Discussionmentioning

confidence: 99%

Cohesin gene defects may impair sister chromatid alignment and genome stability in Arabidopsis thaliana

et al. 2009

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“…20 In vitro Cdk1/ Recombinant Sororin WT and S21A mutant were phosphorylated by Cdk1/cyclin B, and the phosphorylated sites were identified by mass spectrometry. 70 The total number of peptides for each site is shown in the table. A total of 17 putative phosphorylation sites were identified, and 13 were verified in Sororin WT.…”

Section: Sororin Is Posttranslationally Modified By Phosphorylationmentioning

confidence: 99%

“…1,[67][68][69][70] Here we use the handcuff model 71,72 to summarize the role of Sororin in the maintenance and resolution of sister chromatid cohesion (Fig. 2).…”

Section: The Function Of Sororin In Cohesin Cyclementioning

confidence: 99%

Sororin is a master regulator of sister chromatid cohesion and separation

Zhang

Pati

2012

Cell Cycle

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“…In addition, cohesion establishing factors interact with components of the replication machinery such as the proliferating cell nuclear antigen (PCNA), the replication factor C (RFC) [Guacci, 2007;Skibbens et al, 2007] and the origin recognition complex (ORC) [Díaz-Martínez et al, 2008] in yeast. A. thaliana ETG1, a replisome complex protein [Takahashi et al, 2008], and CTF18 are both needed to establish cohesion along chromosome arms but not at centromeres [V. Schubert, N. Takahashi, L. De Veylder, unpublished results].…”

Section: Proteins Interacting With Smc Complexesmentioning

confidence: 99%

SMC Proteins and Their Multiple Functions in Higher Plants

Schubert¹

2009

Cytogenet Genome Res

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In plants as in other eukaryotes, SMC (Structural Maintenance of Chromosome) protein complexes and proteins interacting with them are essential for sister chromatid cohesion, chromosome condensation, DNA repair and recombination. The presence of paralogous genes for various components of the different SMC complexes allowed diversification of their biological functions during evolution of higher plants. Here I summarize the multiple functions of plant SMC complexes and some of the particularities these proteins show in comparison to those of other organisms.

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Fork it over: the cohesion establishment factor Ctf7p and DNA replication

Cited by 33 publications

References 52 publications

Cohesin gene defects may impair sister chromatid alignment and genome stability in Arabidopsis thaliana

Cohesin gene defects may impair sister chromatid alignment and genome stability in Arabidopsis thaliana

Sororin is a master regulator of sister chromatid cohesion and separation

SMC Proteins and Their Multiple Functions in Higher Plants

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