Formaldehyde in Hospitals Induces Oxidative Stress: The Role of GSTT1 and GSTM1 Polymorphisms

Ghelli, Federica; Bellisario, Valeria; Squillacioti, Giulia; Panizzolo, Marco; Santovito, Alfredo; Bono, Roberto

doi:10.3390/toxics9080178

Cited by 5 publications

(4 citation statements)

References 74 publications

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“…In our study we found non-significant (p>0.05) association of GSTM1 and GSTT1 with oxidative stress and contradicting our findings (Ercegovac et al, 2015). According to several researches, deletion of the GSTT1 and GSTM1 genes is linked to an increase in lipid peroxidation indicators such MDA (Datta et al, 2010;Kumar et al, 2012;Ghelli et al, 2021).…”

Section: Influence Of Gstm1 and Gstt1 Genotype On Gsh And Gst Activitycontrasting

confidence: 96%

Interaction of glutathione S- transferase M1 and T1 gene polymorphism and oxidative stress associated biomarkers (GSH and GST) in pesticide exposed workers in Himachal Pradesh

Hemlata¹,

Rani²,

Bhardwaj³

et al. 2023

EEC

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Humans get oxidative stress as a result of using several pesticides and herbicides in excess. Occupational and environmental exposures harm the public’s health even if their effectiveness has led to their widespread use. The current study’s goal was to assess the activity of the antioxidants glutathione reductase and glutathione S-transferase in a group of HP, India residents who had been exposed to pesticides. Pesticide exposed individuals had their glutathione transferase (GSTM1 and GSTT1) genetic polymorphisms investigated to check for any connections to oxidative stress and toxicity. For the genotyping of GSTM1, GSTT1, and antioxidant enzymatic assays such as GSH and GST, 5 ml of blood was drawn from the population under study. Antioxidant enzymatic activity of GSH (205.63 ± 42.42) and GST (1.65 ± 0.39) were decreased in pesticide exposed group as compared to unexposed group GSH (273.76 ± 56.70) and GST (2.40 ± 0.81). We observed non-significant (p>0.05) results for the association of GSTM1 with GSH and GST enzyme activity as well as similar result have been observed for the association of GSTT1 with GSH and GST activity in the studied population. According to the study, prolonged pesticide exposure results in oxidative stress, which is connected to the necessary genetic variance. The findings may pave the way for further investigation into the toxicogenetics and health impacts of pesticides.

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Section: Influence Of Gstm1 and Gstt1 Genotype On Gsh And Gst Activitycontrasting

confidence: 96%

Interaction of glutathione S- transferase M1 and T1 gene polymorphism and oxidative stress associated biomarkers (GSH and GST) in pesticide exposed workers in Himachal Pradesh

Hemlata¹,

Rani²,

Bhardwaj³

et al. 2023

EEC

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“…Several research proved formaldehyde significantly decreased superoxide dismutase and glutathione peroxidase, increased lipid peroxidation with malondialdehyde formation, increased isoprostane levels, and TNF-α, as well as there was a dosing response relationship between lipid peroxidation index and formaldehyde concentration. 8,14 Rat exposed to formaldehyde inhalation showed higher MDA values than the control group, while Superoxide Dismutase (SOD) values were lower than those of the control group. There is a dose-response relationship between formaldehyde concentration and lipid peroxidation index.…”

Section: Introductionmentioning

confidence: 98%

Supplementation of Caesalpinia Sappan L. Extract to Increase Superoxide Dismutase Activity and Suppress Malondialdehyde Levels in Sprague Dawley Exposed to Inhaled Formaldehyde

Nurullita,

Susilaningsih,

Suwondo

et al. 2023

Pharmacogn J.

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Introduction:It is not yet known how Caesalpinia sappan L. extract (CSE) affects the activity of SOD and MDA levels in rats exposed to inhaled formaldehyde. Method: This true experimental study in vivo uses a randomized post-test-only control group design. The subjects were male Sprague Dawley, 2-4 months old, weight 200-300 grams, a total of 30 heads divided into 6 experimental groups. The treatment group was given CSE at doses of 0, 100, 400, and 1000 g/kg BW for 28 days, and exposed to inhaled formaldehyde for 2 weeks, and 8 hours/day. SOD and MDA were measured using the ELISA kit. Statistical analysis used Kruskall Wallis, Mann Whitney, and rank spearman tests with α= 5%. Results: The highest average SOD was in the CSE 1000 group and the lowest was in the negative control group. The highest average MDA levels were in the negative control group and the lowest was in the positive control group. The difference in SOD levels between groups with a p-value = 0.016, while the difference in MDA between groups has a p-value of 0.915. the p-value of the relationship between SOD levels and MDA levels is 0.02 (correlation coefficient = -0.422). Conclusion: Caesalpinia sappan L. extract can increase the activity of superoxide dismutase enzymes but has not been able to suppress malondialdehyde levels. There was an association of increased superoxide dismutase enzyme activity with decreased malondialdehyde levels in Sprague Dawley exposed to inhaled formaldehyde

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“…However, Ghelli et al. ( 27 ) found that GSTT1 gene polymorphisms played a crucial role in the damage to oxidative stress in formaldehyde-induced oxidative stress. Through the above research, we believe that genes GPX3 and GSTT1 play an important role in the oxidative stress responses of tissues and organs, which may be potential targets for regulating oxidative stress during RIRIs.…”

Section: Discussionmentioning

confidence: 99%

GPX3 and GSTT1 as biomarkers related to oxidative stress during renal ischemia reperfusion injuries and their relationship with immune infiltration

Pei

Tian

et al. 2023

Front. Immunol.

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BackgroundRenal ischemia reperfusion injuries (IRIs) are very common in clinical diagnoses and treatments, which are a common cause of impaired renal functions, worsening pathological damage, affecting disease progression and hindering recovery. Renal IRIs are an inflammatory disease mediated by the adaptive and innate immune system. There is a complex interaction between oxidative stress and immune cell infiltration. Therefore, we aimed to determine biomarkers associated with oxidative stress during renal IRIs and their relationship with immune cell infiltration.MethodA differential gene expression analysis was made based on the GSE148420 dataset from the NCBI Gene Expression Comprehensive Database (GEO) combined with 92 oxidative-stress (OS)-related genes identified in the Molecular Signatures Database. Then we identified differentially-expressed genes (DEOSGs) associated with oxidative stress, which were used for gene ontology (GO) and a Kyoto Encyclopedia of Genomes (KEGG) enrichment analysis. At the same time, we used PPI protein interaction networks and Lasso regression analysis to identify key genes, which were verified by the validation sets GSE58438 and GSE71647, as well as Western Blot detection on rat renal IRI models. At the same time, PAS staining, HE staining and immunohistochemistry were used to detect tissue damage and expression of markers related to oxidative stress during renal ischemia-reperfusion. Single-gene enrichment analysis (GSEA) was used to further clarify the underlying biological functions of key genes. Cibersort was used to analyze the immune cell infiltration during renal IRI and the correlation of key genes with immune cells. At the same time, we constructed a network of transcription-factor (TF)-Hub genes and miRNA-Hub genes. DGIDB was used to predict drugs and molecular compounds that might interact with the Hub genes.ResultsCompared with the control group, a total of 5456 differential genes (DEGs) were measured in the renal IRI group, 2486 of which were upregulated and 2970 were down-regulated. Among them, we found 30 DEGs (DEOSGs) associated with oxidative stress. The results of GO and KEGG enrichment analysis showed that these DEOSGs were mainly enriched in glutathione metabolism, the response to oxidative stress stimulation, the regulation of T cell activation and apoptosis signaling pathways. Through a protein interaction network (PPI) and a LASSO regression analysis, a total of two Hub genes were identified, namely GPX3 and GSTT1, which were validated through external validation sets and animal experiments. Through pathological methods, we found that the pathological damage of renal tissue and the expression of oxidative stress markers increased after renal ischemia-reperfusion. The results of GSEA showed that the Hub genes were related to oxidative stress pathways, apoptosis signaling pathways and immune-response-related signaling pathways. An immunoinfiltration correlation analysis showed that genes GPX3 and GSTT1 were significantly positively correlated with plasma cells and macrophage M0, while were negatively correlated with monocytes and macrophages M1 and M2. Using the Strust, Starbase and DGIDB database, we predicted that 81 transcription factors, 49 miRNAs and 13 drug or molecular compounds might interact with the Hub genes.ConclusionThrough a comprehensive analysis of gene expression, our findings may provide new potential biomarkers for the pathogenesis of renal IRIs and a reliable basis for its early diagnosis as well as treatment.

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Formaldehyde in Hospitals Induces Oxidative Stress: The Role of GSTT1 and GSTM1 Polymorphisms

Cited by 5 publications

References 74 publications

Interaction of glutathione S- transferase M1 and T1 gene polymorphism and oxidative stress associated biomarkers (GSH and GST) in pesticide exposed workers in Himachal Pradesh

Interaction of glutathione S- transferase M1 and T1 gene polymorphism and oxidative stress associated biomarkers (GSH and GST) in pesticide exposed workers in Himachal Pradesh

Supplementation of Caesalpinia Sappan L. Extract to Increase Superoxide Dismutase Activity and Suppress Malondialdehyde Levels in Sprague Dawley Exposed to Inhaled Formaldehyde

GPX3 and GSTT1 as biomarkers related to oxidative stress during renal ischemia reperfusion injuries and their relationship with immune infiltration

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