Formaldehyde Oxidizing Enzymes and Genetically Modified Yeast Hansenula polymorpha Cells in Monitoring and Removal of Formaldehyde

Sibirny, Vladimir; Demkiv, Olha; Sigawi, Sasi; Paryzhak, Solomiya; Klepach, Halyna; Korpan, Yaroslav I.; Smutok, Oleh; Nisnevich, Marina; Gayda, Galina; Nitzan, Yeshayahu; Puchalski, Czesław; Gonchar, Mykhailo

doi:10.5772/16148

Cited by 5 publications

(3 citation statements)

References 105 publications

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“…As shown for other enzymes from thermotolerant yeast H. polymorpha, their immobilization opens extensive possibilities for the construction of very sensitive and stable biosensors for wide biochemical applications. [19][20][21] A high thermostability of immobilized on NPs arginase I preparations and a wide working pH range of this enzyme allows to hope that it will become a prоspective tool for bioanalytical purposes, namely, for arginine monitoring in pharmaceuticals, in food products and in blood.…”

Section: Discussionmentioning

confidence: 99%

Recombinant human arginase I immobilized on gold and silver nanoparticles: preparation and properties

et al. 2011

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Metal nanoparticles (NPs), such as gold (Au) and silver (Ag), are important for chemistry, physics, and biology due to their unique optical, electrical, and photothermal properties. Such NPs are widely used for immobilization of various bioactive substances, including peptides, enzymes, antibodies and DNA. The synthesis of silver and gold nanoparticles was carried out by reduction of silver nitrate by glucose and reduction of tetrachloroauric acid by sodium citrate, respectively. The size and structure of the AgNPs and AuNPs were characterized using TEM, AFM and XRD methods. The average size of the AgNPs and AuNPs was between 8 and 15 nm. Recombinant arginase I was immobilized using the carbodiimidepentafluorophenol method on the surface of NPs functionalized with ω-mercaptohexadecanoic acid. It was shown that recombinant human liver arginase I isolated from the yeast Hansenula polymorpha maintains satisfactory stability after immobilization on both NPs. The immobilized arginase retained 40% of its activity on the surface of AuNPs and 25% on AgNPs compared to the free arginase after storage at +4 ºC during 25 days. The immobilized enzyme can be used for assay of arginine in pharmaceuticals, in food products and in blood

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Section: Discussionmentioning

confidence: 99%

Recombinant human arginase I immobilized on gold and silver nanoparticles: preparation and properties

et al. 2011

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“…Formaldehyde, though important in many aspects of human life and endeavours, can have negative effects on human health. Formaldehyde can affect human health, particularly on the immune, blood, and central nervous systems [ 21 ]. Formaldehyde can enter the human body through ingestion, inhalation, and dermal contact.…”

Section: Introductionmentioning

confidence: 99%

Risk Assessment of Kumasi Metropolis Population in Ghana through Consumption of Fish Contaminated with Formaldehyde

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et al. 2018

Journal of Toxicology

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This study evaluates the exposure of the Ghanaian population of the Kumasi Metropolis of Ghana to formaldehyde through the consumption of fish using 3-Methyl-2-Benzothiazoline Hydrazone method, with trichloroacetic acid as an extracting agent. A total of sixty (60) fish species comprising both local and imported fish were bought from cold stores and fish ponds were analysed. Formaldehyde was found in all the species analysed with concentration ranging from 0.174 to 3.710 μgg−1. However, the levels were still lower than 5 mg/kg, which is the maximum limit established by the Malaysian Food Act and Regulation for formaldehyde in fish. The estimated daily intake values for formaldehyde in the fish species analysed ranged between 4.233 × 10−4 and 3.661 × 10−3 mg/kg BW/day and this was less than the acceptable daily intake of 0.15 and 0.2 mg/kg BW/day suggested by World Health Organization and the United States Environmental Protection Agency for formaldehyde intake, respectively. The results for the hazard quotient calculated for all the species were less than one suggesting that the amount of formaldehyde in the fish is not likely to pose any potential adverse health effects to consumers. Thus, wet fish from Kumasi may be considered safe for consumption because of low formaldehyde content.

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“…Different approaches to analysis of this compound, including enzymatic and biosensor techniques [ 18 – 27 ], have been increasingly applied. A chemo- or biosensor (sensor analyzer) analyzer is an integral autonomic analytical device for quantitative or semi-quantitative analysis that uses a selective element, which is in direct contact with a physical transducer [ 23 ]. The selective element is usually placed/immobilized on the surface of the transducer.…”

Section: Introductionmentioning

confidence: 99%

Detection of Waterborne and Airborne Formaldehyde: From Amperometric Chemosensing to a Visual Biosensor Based on Alcohol Oxidase

et al. 2014

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A laboratory prototype of a microcomputer-based analyzer was developed for quantitative determination of formaldehyde in liquid samples, based on catalytic chemosensing elements. It was shown that selectivity for the target analyte could be increased by modulating the working electrode potential. Analytical parameters of three variants of the amperometric analyzer that differed in the chemical structure/configuration of the working electrode were studied. The constructed analyzer was tested on wastewater solutions that contained formaldehyde. A simple low-cost biosensor was developed for semi-quantitative detection of airborne formaldehyde in concentrations exceeding the threshold level. This biosensor is based on a change in the color of a solution that contains a mixture of alcohol oxidase from the yeast Hansenula polymorpha, horseradish peroxidase and a chromogen, following exposure to airborne formaldehyde. The solution is enclosed within a membrane device, which is permeable to formaldehyde vapors. The most efficient and sensitive biosensor for detecting formaldehyde was the one that contained alcohol oxidase with an activity of 1.2 U·mL−1. The biosensor requires no special instrumentation and enables rapid visual detection of airborne formaldehyde at concentrations, which are hazardous to human health.

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Formaldehyde Oxidizing Enzymes and Genetically Modified Yeast Hansenula polymorpha Cells in Monitoring and Removal of Formaldehyde

Cited by 5 publications

References 105 publications

Recombinant human arginase I immobilized on gold and silver nanoparticles: preparation and properties

Recombinant human arginase I immobilized on gold and silver nanoparticles: preparation and properties

Risk Assessment of Kumasi Metropolis Population in Ghana through Consumption of Fish Contaminated with Formaldehyde

Detection of Waterborne and Airborne Formaldehyde: From Amperometric Chemosensing to a Visual Biosensor Based on Alcohol Oxidase

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