Formation and activation induction of primordial follicles using granulosa and cumulus cells conditioned media

Atrabi, Mohammad Jafari; Akbarinejad, Vahid; Khanbabaee, Ramazan; Dalman, A; Amorim, Christiani Andrade; Najar-Asl, Mustafa; Valojerdi, Mojtaba Rezazadeh; Fathi, Rouhollah

doi:10.1002/jcp.27681

Cited by 11 publications

(6 citation statements)

References 53 publications

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“…IMPM, intact mesenteric peritoneal membrane; DMPM, decellularized mesenteric peritoneal membrane. Asterisk, presence of the cells; arrow, collagen fibers before and after decellularization ECM-based scaffolds, 17 co-culture, 36,45 and different conditioned media derived from somatic cells 46 on in vitro follicular culture outcomes, there have, hitherto, been no studies on the use of DMPM as a scaffold and peritoneal mesothelial cells as supportive elements for in vitro development of ovarian follicles. In the current reported work, the peritoneum has been studied for the first time as a supportive structure for enabling in vitro growth of ovarian follicles.…”

Section: Discussionmentioning

confidence: 99%

“…Many earlier studies have also reported the positive effects of co-culture and conditioned medium on development of ovarian follicles. 10,36,46,59,60 These results may be related to the positive effects of the secretions of mesothelial stem cells (as LIF, EGF, TGFβ, Kitligand, and bFGF), 40 which suggests that these cells have secreted factors to promote in vitro growth of the follicles.…”

Section: Discussionmentioning

confidence: 99%

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Co‐culture with peritoneum mesothelial stem cells supports the in vitro growth of mouse ovarian follicles

Sarabadani

Tavana

Mirzaeian

et al. 2021

J Biomedical Materials Res

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The important roles played by the ovarian microenvironment and cell interactions in folliculogenesis suggest promising approaches for in vivo growth of ovarian follicles using appropriate scaffolds containing suitable cell sources. In this study, we have investigated the growth of early preantral follicles in the presence of decellularized mesenteric peritoneal membrane (MPM), peritoneum mesothelial stem cells (PMSCs), and conditioned medium (CM) of PMSCs. MPM of mouse was first decellularized; PMSCs were isolated from MPM and cultured and their conditioned medium (CM) was collected. Mouse follicles were separated into four groups: (1) culture in base medium (control), (2) culture in decellularized MPM (DMPM), (3) co-culture with PMSCs (Co-PMSCs), and (4) culture in CM of PMSCs (CM-PMSCs). Qualitative and quantitative assessments were performed to evaluate intact mesenteric peritoneal membrane (IMPM) as well as decellularized ones. After culturing the ovarian follicles, follicular and oocyte diameter, viability, eccentric oocyte percentage, and estradiol hormone amounts were evaluated. Quantitative and qualitative evaluations confirmed removal of cells and retention of the essential fibers in MPM after the decellularization process. Follicular parameters showed that Co-PMSCs better support in vitro growth and development of ovarian follicles than the other groups. The eccentric rate and estradiol production were statistically higher for the Co-PMSCs group than for the CM-PMSCs and control groups. Although the culture of early preantral follicles on DMPM and CM-PMSCs could improve in vitro follicular growth, co-culture of follicles with PMSCs showed even greater improvements in terms of follicular growth and diameter. K E Y W O R D S conditioned medium, decellularized ECM, mesenteric peritoneal membrane, mesothelial stem cells, ovarian follicle culture | INTRODUCTIONChemo-and radiation therapies lead to early menopause, premature ovarian insufficiency (POI), and/or infertility in women. 1,2 One solution to this problem is the transplantation of fresh or frozen ovaries and ovarian tissues, but the risks of recurrence of malignancy in the cells and allogenic rejection of transplants have led to interest in the development of alternate techniques such as in vitro culture of isolated ovarian follicles. [2][3][4][5] Current follicular culture systems are inefficient due to inconsistent development of both somatic and germinal components of the ovarian follicles. 6 Moreover, in vitro cultured follicles have much smaller diameters and are poorer in quality than in vivo follicles. 7 There have been many studies in recent years aimed at improving in vitro follicle development through the use of various growth factors, supplements, and serum components. 6,8 The use of different matrices and co-cultures with supportive somatic cells and/or their

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Co‐culture with peritoneum mesothelial stem cells supports the in vitro growth of mouse ovarian follicles

Sarabadani

Tavana

Mirzaeian

et al. 2021

J Biomedical Materials Res

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“…One-day-old mice ovaries were cultured for six days with conditioned media of GCs or CCs or co-cultured with these cells. Obtained results indicated that the conditioned medium of GCs could contribute to primordial follicle activation, probably due to downregulation of PTEN [147].…”

Section: Application Of Ovarian Follicular Stem Cells In Translational Medicinementioning

confidence: 95%

“…Therefore, the supernatant of cumulus-granulosa cells could be a useful IVF-enhancing tool [146]. Atrabi et al [147] investigated the influence of the conditioned medium of GCs and CCs on the activation of primordial follicles in mice. One-day-old mice ovaries were cultured for six days with conditioned media of GCs or CCs or co-cultured with these cells.…”

Section: Application Of Ovarian Follicular Stem Cells In Translational Medicinementioning

confidence: 99%

Human Granulosa Cells—Stemness Properties, Molecular Cross-Talk and Follicular Angiogenesis

Dompe

Kulus

Stefańska

et al. 2021

Cells

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The ovarian follicle is the basic functional unit of the ovary, comprising theca cells and granulosa cells (GCs). Two different types of GCs, mural GCs and cumulus cells (CCs), serve different functions during folliculogenesis. Mural GCs produce oestrogen during the follicular phase and progesterone after ovulation, while CCs surround the oocyte tightly and form the cumulus oophurus and corona radiata inner cell layer. CCs are also engaged in bi-directional metabolite exchange with the oocyte, as they form gap-junctions, which are crucial for both the oocyte’s proper maturation and GC proliferation. However, the function of both GCs and CCs is dependent on proper follicular angiogenesis. Aside from participating in complex molecular interplay with the oocyte, the ovarian follicular cells exhibit stem-like properties, characteristic of mesenchymal stem cells (MSCs). Both GCs and CCs remain under the influence of various miRNAs, and some of them may contribute to polycystic ovary syndrome (PCOS) or premature ovarian insufficiency (POI) occurrence. Considering increasing female fertility problems worldwide, it is of interest to develop new strategies enhancing assisted reproductive techniques. Therefore, it is important to carefully consider GCs as ovarian stem cells in terms of the cellular features and molecular pathways involved in their development and interactions as well as outline their possible application in translational medicine.

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“…In prepubertal cancer patients, ovarian tissue could be cryopreserved and autotransplanted following recovery, but contamination of cryopreserved tissue with malignant cells and cancer reoccurrence are the risks pertaining to this technique (Bockstaele et al, 2012;Xiao et al, 2015;Amorim and Shikanov, 2016). Alternatively, cryopreserved ovarian tissue could be cultured to activate primordial follicles, which could further be used for in vitro production of embryos (Telfer et al 2008;Amorim and Shikanov, 2016;McLaughlin et al, 2018;Atrabi et al, 2019). Although in vitro activation of primordial follicles and in vitro production of metaphase II oocyte have been achieved successfully in postpubertal women (Telfer et al, 2008;McLaughlin et al, 2018), in vitro activation of primordial follicles in prepubertal girls remains to be defined (Telfer et al, 2008;Luyckx et al, 2013;Telfer and Zelinski, 2013).…”

Section: Introductionmentioning

confidence: 99%

Incorporation of arginine, glutamine or leucine in culture medium accelerates in vitro activation of primordial follicles in 1-day-old mouse ovary

Alborzi

Atrabi

Akbarinejad

et al. 2020

Zygote

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Summary In vitro activation of primordial follicles provides cancer patients subjected to oncotherapy with a safe therapeutic strategy for fertility preservation, however a successful protocol for activation of primordial follicles in prepubertal patients has not yet been defined comprehensively. There is evidence that amino acids such as leucine, arginine and glutamine could stimulate the mammalian target of rapamycin (mTOR) pathway, which plays a pivotal role in primordial follicle activation. Nevertheless, there has been no report that elucidates the effect of these amino acids on in vitro development of ovarian follicles. Therefore, the present study was conducted to evaluate the effects of these amino acids and their combination on the formation and activation of primordial follicles in 1-day-old murine ovaries during an 11-day culture period. The experimental groups consisted of base medium (BM), base medium + arginine (ARG), base medium + glutamine (GLU), base medium + leucine (LEU) and base medium + a combination of arginine, glutamine and leucine (AGL). The proportions of different stages of ovarian follicles and gene expression of regulatory factors were assessed using histology and quantitative real-time PCR on days 5 and 11 of culture. The proportion of transitional and primary follicles was greater in all amino acid-treated groups compared with the BM group (P < 0.05). Moreover, leucine resulted in elevated expression of Gdf9 and Bmp15, and glutamine augmented the expression of Pi3k on day 11 of culture. In conclusion, the present study showed that inclusion of leucine, glutamine, arginine or their combination in the culture medium for murine ovarian tissue could accelerate the activation of primordial follicles and alter the expression of the corresponding factors.

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Formation and activation induction of primordial follicles using granulosa and cumulus cells conditioned media

Cited by 11 publications

References 53 publications

Co‐culture with peritoneum mesothelial stem cells supports the in vitro growth of mouse ovarian follicles

Co‐culture with peritoneum mesothelial stem cells supports the in vitro growth of mouse ovarian follicles

Human Granulosa Cells—Stemness Properties, Molecular Cross-Talk and Follicular Angiogenesis

Incorporation of arginine, glutamine or leucine in culture medium accelerates in vitro activation of primordial follicles in 1-day-old mouse ovary

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