Formation and characterization of (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine)/(1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine) supported lipid bilayers on polyelectrolyte multilayer films

Wlodek, Magdalena; Kolasińska-Sojka, Marta; Szuwarzyński, Michał; Zapotoczny, Szczepan; Warszyński, Piotr

doi:10.1016/j.tsf.2015.08.050

Cited by 6 publications

(5 citation statements)

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“…Moreover, hydrogen bonding formation among PE lipids and between the PE headgroup and the solid support could promote vesicle fusion and SLB formation. 44,45 The PEI film has been previously shown [46][47][48][49][50] as an effective anchor for subsequent adsorption of nanoobjects such as polyelectrolytes, nanoparticles, or liposomes. In our study, the PEI monolayer preparation used the same procedure as that in these studies, and the detailed characterization of such PEI monolayers has also been reported by Adamczyk et al.…”

Section: 20mentioning

confidence: 99%

Supported lipid bilayers with encapsulated quantum dots (QDs) via liposome fusion: effect of QD size on bilayer formation and structure

et al. 2018

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Understanding interactions between functional nanoparticles and lipid bilayers is important to many emerging biomedical and bioanalytical applications. In this paper, we report incorporation of hydrophobic cadmium sulphide quantum dots (CdS QDs) into mixed 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) liposomes, and into their supported bilayers (SLBs). The QDs were found embedded in the hydrophobic regions of the liposomes and the supported bilayers, which retained the QD fluorescent properties. In particular, we studied the effect of the QD size (2.7-5.4 nm in diameter) on the formation kinetics and structure of the supported POPC/POPE bilayers, monitored in situ using quartz crystal microbalance with dissipation monitoring (QCM-D), as the liposomes ruptured onto the substrate. The morphology of the obtained QD-lipid hybrid bilayers was studied using atomic force microscopy (AFM), and their structure by synchrotron X-ray reflectivity (XRR). It was shown that the incorporation of hydrophobic QDs promoted bilayer formation on the PEI cushion, evident from the rupture and fusion of the QD-endowed liposomes at a lower surface coverage compared to the liposomes without QDs. Furthermore, the degree of disruption in the supported bilayer structure caused by the QDs was found to be correlated with the QD size. Our results provide mechanistic insights into the kinetics of the rupturing and formation process of QD-endowed supported lipid bilayers via liposome fusion on polymer cushions.

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Section: 20mentioning

confidence: 99%

Supported lipid bilayers with encapsulated quantum dots (QDs) via liposome fusion: effect of QD size on bilayer formation and structure

et al. 2018

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“…A recent experiment reported that on PEM cushions composed of poly- l -lysine (PLL) hydrobromide and poly- l -glutamic acid (PGA) sodium, vesicle adsorption and rupture depended on the thickness of the cushion. 24 In that experiment, a multilayered PLL/PGA mixture was adsorbed on a surface that was coated by positively charged branched poly(ethyleneimine) (PEI). The SLB formation was observed only at surfaces covered with a thin polycation film, whereas the deposition of intact vesicles was observed on a thick multilayer cushion.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Formation Mechanism and Properties of Polyelectrolyte Multilayer-Supported Lipid Bilayers: A Coarse-Grained Molecular Dynamics Study

Wen

Wan

et al. 2017

ACS Omega

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Polyelectrolyte multilayer (PEM)-supported lipid bilayers (SLBs) that connect with functional proteins are popular models for cell membranes and are usually obtained via vesicle adsorption and spreading. However, the exact mechanism by which SLBs are formed is not fully understood. In this study, we employ coarse-grained molecular dynamics simulations to investigate the pathways by which vesicles undergo spreading upon the deposition on PEM-cushioned substrates. The substrates consist of positive chitosan (CHI)/negative alginate (ALG) multilayers. We find that an isolated vesicle tends to completely disintegrate upon deposition, forming a well-ordered lipid bilayer at appropriate polymer ionic strengths by a mechanism described as “parachute” model. Lipids from the vesicle’s outer leaflet are predominantly oriented toward the bulk after the formation of the SLB. The PEM cushion provides adsorption energy of 26.9 kJ mol –1 per lipid for the SLBs. The process by which SLBs are formed is almost independent of the number of layers of CHI/ALG in the PEM cushion. Additional simulations on vesicle clusters also demonstrate that the formation of SLBs can be catalyzed by either neighboring vesicles or preexisting bilayer edges on the support. Moreover, our simulations show that SLBs created on PEM supports preserve the lateral mobility and the symmetric density profile of the phospholipids, as in a freestanding bilayer.

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“…These limitations can be overcome by increasing the thickness of the aqueous lubricant layer by introducing a soft hydrophilic polymer network or polyelectrolyte film (10–100 nm) between the membrane bilayer and the solid substrate to maintain both the dynamic properties of the membrane and the structural integrity of the functional protein–membrane assembly (Figure G). − The polymer film, which acts as a spacer between solid substrate and the lipid bilayer, minimizes negative substrate effects, such as defect formation, decreased lateral mobility, and limited self-annealing of the membrane. Furthermore, the polymer interface allows the incorporation of transmembrane proteins in their native form with the surface domains protruding into the hydrophilic polymer network. ,,− The dynamic properties of lipids and the incorporated molecules were also demonstrated by the remarkable long-range lateral mobility of lipopeptides embedded into a polymer-supported bilayer .…”

Section: Model Membrane Systemsmentioning

confidence: 99%

Exploring Molecular-Biomembrane Interactions with Surface Plasmon Resonance and Dual Polarization Interferometry Technology: Expanding the Spotlight onto Biomembrane Structure

et al. 2018

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The molecular analysis of biomolecular-membrane interactions is central to understanding most cellular systems but has emerged as a complex technical challenge given the complexities of membrane structure and composition across all living cells. We present a review of the application of surface plasmon resonance and dual polarization interferometry-based biosensors to the study of biomembrane-based systems using both planar mono- or bilayers or liposomes. We first describe the optical principals and instrumentation of surface plasmon resonance, including both linear and extraordinary transmission modes and dual polarization interferometry. We then describe the wide range of model membrane systems that have been developed for deposition on the chips surfaces that include planar, polymer cushioned, tethered bilayers, and liposomes. This is followed by a description of the different chemical immobilization or physisorption techniques. The application of this broad range of engineered membrane surfaces to biomolecular-membrane interactions is then overviewed and how the information obtained using these techniques enhance our molecular understanding of membrane-mediated peptide and protein function. We first discuss experiments where SPR alone has been used to characterize membrane binding and describe how these studies yielded novel insight into the molecular events associated with membrane interactions and how they provided a significant impetus to more recent studies that focus on coincident membrane structure changes during binding of peptides and proteins. We then discuss the emerging limitations of not monitoring the effects on membrane structure and how SPR data can be combined with DPI to provide significant new information on how a membrane responds to the binding of peptides and proteins.

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Formation and characterization of (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine)/(1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine) supported lipid bilayers on polyelectrolyte multilayer films

Cited by 6 publications

References 54 publications

Supported lipid bilayers with encapsulated quantum dots (QDs) via liposome fusion: effect of QD size on bilayer formation and structure

Supported lipid bilayers with encapsulated quantum dots (QDs) via liposome fusion: effect of QD size on bilayer formation and structure

Formation Mechanism and Properties of Polyelectrolyte Multilayer-Supported Lipid Bilayers: A Coarse-Grained Molecular Dynamics Study

Exploring Molecular-Biomembrane Interactions with Surface Plasmon Resonance and Dual Polarization Interferometry Technology: Expanding the Spotlight onto Biomembrane Structure

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