Formation and characterization of spontaneously formed heterokaryons between quail myoblasts and 3T3-L1 preadipocytes: correlation between differential plasticity and degree of differentiation

Sasao, Nagako; Hirayama, Etsuko; Kim, Jeman

doi:10.1078/0171-9335-00357

Cited by 4 publications

(3 citation statements)

References 54 publications

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“…Our results have shown that 3T3‐L1 adipocytes suppressed the differentiation of C2C12 muscle cells through downregulation of myogenin and upregulation of myostatin and atrophy‐related ubiquitin E3 ligases, Atrogin‐1, and MuRF‐1. Previously published results using a co‐culture system have shown that adipocytes inhibit muscle cell fusion (Pellegrinelli et al., ; Sasao, Hirayama, & Kim, ; Takegahara et al., ), and reduce the thickness of myotubes (Pellegrinelli et al., ). Takegahara et al.…”

Section: Discussionmentioning

confidence: 99%

“…Our results have shown that 3T3-L1 adipocytes suppressed the differentiation of C2C12 muscle cells through downregulation of myogenin and upregulation of myostatin and atrophy-related ubiquitin E3 ligases, Atrogin-1, and MuRF-1. Previously published results using a co-culture system have shown that adipocytes inhibit muscle cell fusion (Pellegrinelli et al, 2015;Sasao, Hirayama, & Kim, 2004;Takegahara et al, 2014), and reduce the thickness of myotubes (Pellegrinelli et al, 2015). Takegahara et al (2014) showed that conditioned media from differentiated mature adipocytes suppressed the fusion of skeletal muscle progenitor cells whereas conditioned media from undifferentiated preadipocytes promoted the fusion of skeletal muscle precursor cells.…”

Section: Discussionmentioning

confidence: 99%

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Adipocytes suppress differentiation of muscle cells in a co‐culture system

Seo

Suzuki

Kobayashi

et al. 2018

Animal Science Journal

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The development of adipose tissue in skeletal muscle is important for improving meat quality. However, it is still unclear how adipocytes grow in the proximity of muscle fibers. We hypothesized that adipocytes would suppress muscle cell growth so as to grow dominantly within muscle. In this study, we investigated the effect of adipocytes on the differentiation of muscle cells in a co‐culture system. The fusion index of C2C12 myoblasts co‐cultured with 3T3‐L1 adipocytes was significantly lower than that of the control. The expression of myogenin and myosin heavy chain in C2C12 muscle cells co‐cultured with 3T3‐L1 adipocytes was significantly lower than in the control. Furthermore, the expression of Atrogin‐1 and MuRF‐1 was higher in C2C12 muscle cells co‐cultured with 3T3‐L1 adipocytes than the control. These results suggest that 3T3‐L1 adipocytes suppress the differentiation of C2C12 myoblasts. In addition, 3T3‐L1 adipocytes induced the expression and secretion of IL‐6 in C2C12 muscle cells. The fusion index and myotube diameter were higher in C2C12 muscle cells co‐cultured with 3T3‐L1 cells in medium containing IL‐6‐neutralizing antibody than the control. Taken together, there is a possibility that adipocyte‐induced IL‐6 expression in muscle cells could be involved in the inhibition of muscle cell differentiation via autocrine.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Adipocytes suppress differentiation of muscle cells in a co‐culture system

Seo

Suzuki

Kobayashi

et al. 2018

Animal Science Journal

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“…Sasao et al (2003) found that, in direct and indirect co‐cultures of the mouse myoblasts, 3T3‐L1 preadipocytes and mature adipocytes, undifferentiated adipocytes have plasticity but terminally differentiated cells do not. Cells can spontaneously fuse with myoblasts under the direct co‐culture conditions and can express myogenin (a sarcoplasm‐specific factor) (Sasao et al, 2004). Our research found that in the co‐culture system of the IMPs and SMSCs, the IMPs differentiated into mature adipocytes, while the ability of the SMSCs to differentiate into muscle cells was diminished, indicating that the undifferentiated adipocytes and muscle cells were plastic and expressed the marker genes during the differentiation.…”

Section: Discussionmentioning

confidence: 99%

Comparison of lipid deposition of intramuscular preadipocytes in Tan sheep co‐cultured with satellite cells or alone

Zhao

et al. 2021

Animal Physiology Nutrition

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The purpose of this study was to investigate the effect of the skeletal muscle satellite cells (SMSCs) on the lipid deposition of the intramuscular preadipocytes (IMPs) in a co‐culture system of the Tan sheep cells. The SMSCs and IMPs from Tan sheep were separated and cultured. After the two kinds of cells were separated and cultured, they were inoculated onto a transwell cell chamber co‐culture plate for co‐cultivation. When the cell density reached more than 90%, the cells were induced to differentiate. After the induction of the SMSCs differentiation for 8 days, the level of the IMPs differentiation and the expression levels of the differentiation marker genes and the key enzymes of the lipid metabolism were assessed. The results showed that the number and area of the lipid droplets in the IMPs in the co‐culture system were significantly reduced compared to those in the IMPs culture alone (p < 0.05). Meanwhile, the expression levels of the PPARγ, c/EBPα, ACC, FAS mRNA in the IMPs were significantly decreased (p < 0.05); the expression level of aP2 mRNA was decreased, but the difference was not significant (p > 0.05).These findings indicate that the SMSCs of the Tan sheep in the co‐culture system inhibited the lipid deposition by the IMPs.

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The proliferation and differentiation characteristics of co-cultured porcine preadipocytes and muscle satellite cells in vitro

et al. 2012

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To explore the proliferation and differentiation characteristics of co-cultured porcine preadipocytes and muscle satellite cells, preadipocytes and muscle satellite cells were isolated from the healthy nascent landrace. Oil Red O stain and desmin immunohistochemistry were used to identify the two solo-cultured cells. Methyl thiazolyl tetrazolium (MTT) colorimetric assay was used to detect the proliferation characteristic of co-cultured cells, and the expression level of differentiation marker genes lipoprotein lipase (LPL), peroxisome proliferator-activated receptors (PPARγ), myogenic factor 5 (Myf5), myogenin (MyoG) were analyzed with reverse transcription PCR (RT-PCR) and western blot. The success of co-culture system was proved. In the co-cultured cells, slight lipid droplets were observed and appeared more slowly. The polykaryocytes fused into myotubes in co-cultured cells were less and relatively slow than that in solo myocytes. After fusion, the proliferation rate of co-cultured cells was higher than that in the solo-cultured muscle satellite cells (P < 0.01), and the duration were also longer. On day 5 and 10, the expression of the marker genes in earlier stage of cell differentiation (LPL and Myf5) were lower than those in the solo-cultured cells (P < 0.01) (except LPL gene at day 5). Moreover, the expression of intermediate and advanced stages' maker genes (PPARγ2 and MyoG) were hardly detectable at day 5, but increased significantly on day 10 (P < 0.01). These results confirm that the co-culture system could facilitate the cells' growth and proliferation, meanwhile, inhibited the cell differentiation.

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Formation and characterization of spontaneously formed heterokaryons between quail myoblasts and 3T3-L1 preadipocytes: correlation between differential plasticity and degree of differentiation

Cited by 4 publications

References 54 publications

Adipocytes suppress differentiation of muscle cells in a co‐culture system

Adipocytes suppress differentiation of muscle cells in a co‐culture system

Comparison of lipid deposition of intramuscular preadipocytes in Tan sheep co‐cultured with satellite cells or alone

The proliferation and differentiation characteristics of co-cultured porcine preadipocytes and muscle satellite cells in vitro

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