Formation and degradation of lipid bodies found in the riboflavin-producing fungus Ashbya gossypii

Stahmann, K.‐Peter; Kupp, C.; Feldmann, Sigrun D.; Sahm, Hermann

doi:10.1007/bf00170234

Cited by 40 publications

(17 citation statements)

References 23 publications

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“…gossypii has been shown to accumulate high amounts of lipid bodies as carbon and energy storage pools (Stahmann et al, 1994;Ledesma-Amaro et al, 2014a). Although the carbon sources utilized play a determinant role in its lipidogenic profile, A. gossypii predominantly accumulates unsaturated fatty acids (FA) (Stahmann et al, 1994;Ledesma-Amaro et al, 2014a).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Ashbya gossypii beyond industrial riboflavin production: A historical perspective and emerging biotechnological applications

Aguiar

Silva

Domingues

2015

Biotechnology Advances

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The filamentous fungus Ashbya gossypii has been safely and successfully used for more than two decades in the commercial production of riboflavin (vitamin B2). Its industrial relevance combined with its high genetic similarity with Saccharomyces cerevisiae together promoted the accumulation of fundamental knowledge that has been efficiently converted into a significant molecular and in silico toolbox for its genetic engineering. This synergy has enabled a directed and sustained exploitation of A. gossypii as an industrial riboflavin producer. Although there is still room for optimizing riboflavin production, the recent years have seen an abundant advance in the exploration of A. gossypii for other biotechnological applications, such as the production of recombinant proteins, single cell oil and flavour compounds. Here, we will address the biotechnological potential of A. gossypii beyond riboflavin production by presenting (a) a physiological and metabolic perspective over this fungus; (b) the molecular toolbox available for its manipulation; and (c) commercial and emerging biotechnological applications for this industrially important fungus, together with the approaches adopted for its engineering.

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Section: Accepted Manuscriptmentioning

confidence: 99%

Ashbya gossypii beyond industrial riboflavin production: A historical perspective and emerging biotechnological applications

Aguiar

Silva

Domingues

2015

Biotechnology Advances

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“…The biomass equation and ATP for maintenance were taken from the iTO977 model, as insufficient information is available from A. gossypii. When oil was used as a carbon source, the biomass equation was modified in lipid composition according to previously reported data (Stahmann et al, 1994).…”

Section: Reconstruction Of Genome-scale Metabolic Modelmentioning

confidence: 99%

Genome scale metabolic modeling of the riboflavin overproducer Ashbya gossypii

Ledesma-Amaro

Kerkhoven

Revuelta

et al. 2013

Biotech & Bioengineering

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Ashbya gossypii is a filamentous fungus that naturally overproduces riboflavin, or vitamin B2. Advances in genetic and metabolic engineering of A. gossypii have permitted the switch from industrial chemical synthesis to the current biotechnological production of this vitamin. Additionally, A. gossypii is a model organism with one of the smallest eukaryote genomes being phylogenetically close to Saccharomyces cerevisiae. It has therefore been used to study evolutionary aspects of bakers' yeast. We here reconstructed the first genome scale metabolic model of A. gossypii, iRL766. The model was validated by biomass growth, riboflavin production and substrate utilization predictions. Gene essentiality analysis of the A. gossypii model in comparison with the S. cerevisiae model demonstrated how the whole-genome duplication event that separates the two species has led to an even spread of paralogs among all metabolic pathways. Additionally, iRL766 was used to integrate transcriptomics data from two different growth stages of A. gossypii, comparing exponential growth to riboflavin production stages. Both reporter metabolite analysis and in silico identification of transcriptionally regulated enzymes demonstrated the important involvement of beta-oxidation and the glyoxylate cycle in riboflavin production.

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“…The presence of lipid bodies in A. gossypii hyphae has been reported previously (21); however, the biotechnological potential of A. gossypii for SCO production has remained unexplored. Here, we analyzed the FA content of a wild-type strain of A. gossypii, and we found that unsaturated FAs represented more than 80% of the total FA composition, suggesting an effective system of FA desaturation in A. gossypii.…”

Section: Discussionmentioning

confidence: 82%

“…A. gossypii is considered to be a model organism for the biotechnological production of vitamins, nucleotides, and proteins (19,29,30). Furthermore, it has been reported that A. gossypii is able to accumulate a significant amount of lipid bodies in its multinucleated hyphae (21) (Fig. 1A).…”

Section: Resultsmentioning

confidence: 99%

“…However, it has been reported that A. gossypii is able to accumulate between 10 and 20% of the cell dry weight as lipid bodies and, moreover, 54% of the total FA composition corresponded to the essential unsaturated omega-6 linoleic acid when soybean oil was used as the sole carbon source (21). In contrast to most bona fide oleaginous microorganisms, A. gossypii lacks any ACL-coding gene, and hence, cytosolic acetylCoA is provided solely by acetyl-CoA synthase (ACS) activity.…”

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confidence: 99%

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Strain Design of Ashbya gossypii for Single-Cell Oil Production

Ledesma-Amaro

Santos

Jiménez

et al. 2014

Appl Environ Microbiol

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Single-cell oil (SCO) represents a sustainable alternative for the oil industry. Accordingly, the identification of microorganisms with either higher lipidogenic ability or novel capacities for the transformation of raw materials constitutes a major challenge for the field of oil biotechnology. With this in mind, here, we were prompted to address the lipidogenic profile of the filamentous hemiascomycete Ashbya gossypii, which is currently used for the microbial production of vitamins. We found that A. gossypii mostly accumulates unsaturated fatty acids (FAs), with more than 50% of the total FA content corresponding to oleic acid. In addition, we engineered A. gossypii strains both lacking the beta-oxidation pathway and also providing ATP-citrate lyase (ACL) activity to block the degradation of FA and to increase the cytosolic acetyl-coenzyme A (CoA) content, respectively. The lipidogenic profile of the newly developed strains demonstrates that the mere elimination of the beta-oxidation pathway in A. gossypii triggers a significant increase in lipid accumulation that can reach 70% of cell dry weight. The use of A. gossypii as a novel and robust tool for the production of added-value oils is further discussed.

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Formation and degradation of lipid bodies found in the riboflavin-producing fungus Ashbya gossypii

Cited by 40 publications

References 23 publications

Ashbya gossypii beyond industrial riboflavin production: A historical perspective and emerging biotechnological applications

Ashbya gossypii beyond industrial riboflavin production: A historical perspective and emerging biotechnological applications

Genome scale metabolic modeling of the riboflavin overproducer Ashbya gossypii

Strain Design of Ashbya gossypii for Single-Cell Oil Production

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