FORMATION BY HYDROGEN PEROXIDE OR 254 nm RADIATION OF A NEAR‐UV CHROMOPHORE FROM PEPTIDE‐BOUND CYSTEINE

McCormick, John; S, Klita; Terry, James A.; Schrodt, Mary; Eisenstark, A.

doi:10.1111/j.1751-1097.1982.tb04388.x

Cited by 4 publications

(4 citation statements)

References 9 publications

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“…Reactions [8] and [9] add together to give reaction [I], in good agreement with the usually accepted overall reaction:…”

Section: Kinetic Schemesupporting

confidence: 77%

“…But the kinetics of the reaction were not studied. The same observation was obtained when aerated aqueous solutions of GSH were irradiated with a 254 nm radiation (9). From several comparative studies with other thiols in the same conditions, they attributed the absorbance at 305 nm to a product which is formed on the N-acetylcysteinamide (-CONH-CH-CONH2) part I CH2-SH of GSH.…”

Section: Introductionsupporting

confidence: 64%

“…Concerning the reaction of H202 with GSH, McCormic et al (9) have shown that a UV chromophore having a maximal absorption at 305 nm appears during the reaction. But the kinetics of the reaction were not studied.…”

Section: Introductionmentioning

confidence: 99%

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Kinetic study of the oxidation mechanism of glutathione by hydrogen peroxide in neutral aqueous medium

Abedinzadeh¹,

Gardès-Albert²,

Ferradini³

1989

Can. J. Chem.

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The oxidation kinetics of glutathione (GSH) by hydrogen peroxide has been studied at neutral pH for different concentration ratios [GSH]0/[H2O2]0 between 0.2 and 2 (5 × 10−4 M ≤ [H2O2]0 ≤ 2.5 × 10−3M; 4 × 10−4 M ≤ [GSH]0 ≤ 2.5 × 10−3 M). In all cases studied, glutathione disulfide GSSG is the main product formed via two different oxidation ways, each of them contributing respectively to 80–85% and 10–15%.Our kinetic data indicate that an important fraction of hydrogen peroxide disappears without oxidizing the thiol function. This can be attributed to a combination between GSH and H2O2 protecting the sulfide group. Chemical evidences of the existence of a peroxide bond with GSSG are described. In our experimental conditions, the overall oxidation equation is 2mol GSH reacting with 2mol H2O2 giving 1mol GSSG. It is very different from the usually accepted stoichiometric reaction:[Formula: see text]A kinetic scheme is proposed and the corresponding rate constants are determined. Keywords: glutathione, hydrogen peroxide, kinetic mechanism, glutathione disulfide.

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“…Reactions [8] and [9] add together to give reaction [I], in good agreement with the usually accepted overall reaction:…”

Section: Kinetic Schemesupporting

confidence: 77%

Section: Introductionsupporting

confidence: 64%

See 1 more Smart Citation

Kinetic study of the oxidation mechanism of glutathione by hydrogen peroxide in neutral aqueous medium

Abedinzadeh¹,

Gardès-Albert²,

Ferradini³

1989

Can. J. Chem.

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“…H202, in turn, was assumed to produce lesions in DNA [Ananthaswamy andEisenstark, 1976, 1977;Ananthaswamy et al, 1979;Hartman, 1986;Hartman andEisenstark, 1978, 1980;Hartman et al, 19793. H202 also may be a photoproduct of cysteine irradiation [Greenberg and Demple, 1986;McCormick et al, 1976McCormick et al, , 1982Owens and Hartman, 19861. We have since found that H202, plus certain wavelengths of NUV, synergistically kill bacteria and phage Hartman and Eisenstark, 1978Hartman et al, 19791. This synergistic action may be the result of NUV conversion of H202 to 0, (superoxide) [Ahmad, 19811, and this action may generate a new NUV photoreceptor [McCormick et al, 19821.…”

Section: Tryptophan Cysteine and Polypeptidesmentioning

confidence: 99%

Mutagenic and lethal effects of near‐ultraviolet radiation (290–400 nm) on bacteria and phage

Eisenstark

1987

Environ and Mol Mutagen

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Despite decades of study of the effect of near-ultraviolet radiation (NUV) on bacterial cells, insights into mechanisms of deleterious alterations and subsequent recovery are just now emerging. These insights are based on observations that 1) damage by NUV may be caused by a reactive oxygen molecule, since H2O2 may be a photoproduct of NUV; 2) some, but not all, of the effects of NUV and H2O2 are interchangeable; 3) there is an inducible regulon (oxyR) that responds to oxidative stress and is involved in protection against NUV; 4) a number of NUV-sensitive mutants are defective either in the capacity to detoxify reactive oxygen molecules or to repair DNA damage caused by NUV; and 5) recovery from NUV damage may not directly involve induction of the SOS response. Since several distinctly different photoreceptors and targets are involved, it is unknown whether NUV lethality and mutagenesis result from an accumulation of damages or whether there is a particularly critical photoeffect. To fully understand the mechanisms involved, it is important to identify the chromophore(s) of NUV, the mechanism of toxic oxygen species generation, the role of the oxidative defense regulon (oxyR), the specific lesions in the DNA, and the enzymatic events of subsequent repair.

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Genetic damage in Salmonella typhimurium by near-ultraviolet radiation

Eisenstark

1983

Mutation Research Letters

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FORMATION BY HYDROGEN PEROXIDE OR 254 nm RADIATION OF A NEAR‐UV CHROMOPHORE FROM PEPTIDE‐BOUND CYSTEINE

Cited by 4 publications

References 9 publications

Kinetic study of the oxidation mechanism of glutathione by hydrogen peroxide in neutral aqueous medium

Kinetic study of the oxidation mechanism of glutathione by hydrogen peroxide in neutral aqueous medium

Mutagenic and lethal effects of near‐ultraviolet radiation (290–400 nm) on bacteria and phage

Genetic damage in Salmonella typhimurium by near-ultraviolet radiation

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