Formation of giant plasma membrane vesicles for biological and medical applications: a review

Li, Yang; Liu, Songyang; Xu, Wei; Wang, Kemin; He, Fengjiao; Li, Jianbo

doi:10.1039/d3sd00060e

Cited by 4 publications

(2 citation statements)

References 68 publications

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“…Giant unilamellar vesicles were prepared at room temperature (23° C) by the electroformation method. [ 17 ] An equimolar solution of azo‐PC and POPC including 0.1 mol% Atto‐647N‐DOPE was prepared in chloroform to a final concentration of 4 m m . In order to create a thin lipid film, 14 µL of this lipid solution was first spread on a pair of electrically conductive, ITO‐coated glass plates and then the majority of the chloroform was evaporated by exposing the plates to a stream of N 2 .…”

Section: Methodsmentioning

confidence: 99%

“…Giant vesicles are cell‐sized, biomembrane compartments with an increasingly broad spectrum of applications. [ 17 ] UV exposure of GUVs composed of 1‐palmitoyl‐2‐oleoyl‐glycero‐3‐phosphocholine (POPC) and photoswitchable azobenzene phospholipid analog (azo‐PC) can trigger a reversible increase in membrane area upon trans ‐to‐ cis isomerization. [ 18 ] Azo‐PC is a commercially available photolipid with a well‐characterized synthetic route.…”

Section: Introductionmentioning

confidence: 99%

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Photoswitchable Endocytosis of Biomolecular Condensates in Giant Vesicles

Mangiarotti,

Aleksanyan,

Siri

et al. 2024

Advanced Science

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Interactions between membranes and biomolecular condensates can give rise to complex phenomena such as wetting transitions, mutual remodeling, and endocytosis. In this study, light‐triggered manipulation of condensate engulfment is demonstrated using giant vesicles containing photoswitchable lipids. UV irradiation increases the membrane area, which can be stored in nanotubes. When in contact with a condensate droplet, the UV light triggers rapid condensate endocytosis, which can be reverted by blue light. The affinity of the protein‐rich condensates to the membrane and the reversibility of the engulfment processes is quantified from confocal microscopy images. The degree of photo‐induced engulfment, whether partial or complete, depends on the vesicle excess area and the relative sizes of vesicles and condensates. Theoretical estimates suggest that utilizing the light‐induced excess area to increase the vesicle‐condensate adhesion interface is energetically more favorable than the energy gain from folding the membrane into invaginations and tubes. The overall findings demonstrate that membrane‐condensate interactions can be easily and quickly modulated via light, providing a versatile system for building platforms to control cellular events and design intelligent drug delivery systems for cell repair.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Photoswitchable Endocytosis of Biomolecular Condensates in Giant Vesicles

Mangiarotti,

Aleksanyan,

Siri

et al. 2024

Advanced Science

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Influence of the glycocalyx on the size and mechanical properties of plasma membrane-derived vesicles

Jani,

Colville,

Park

et al. 2025

Soft Matter

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Photoswitchable endocytosis of biomolecular condensates in giant vesicles

Mangiarotti,

Aleksanyan,

Siri

et al. 2024

Preprint

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Interactions between membranes and biomolecular condensates can give rise to complex phenomena such as wetting transitions, mutual remodeling, and endocytosis. In this study, we demonstrate a light-triggered manipulation of condensate engulfment using giant vesicles containing photoswitchable lipids. UV irradiation increases the membrane area, facilitating a rapid condensate endocytosis, which can be reverted by blue light. The affinity of the protein-rich condensates to the membrane and the reversibility of the engulfment processes is quantified from confocal microscopy images. The degree of engulfment, whether partial or complete, depends on the initial membrane excess area and the relative sizes of vesicles and condensates. Theoretical estimates suggest that utilizing the light-induced excess area to increase the vesicles-condensate adhesion interface is energetically more favorable than the energy gain from folding the membrane into invaginations and tubes. Our overall findings demonstrate that membrane-condensate interactions can be easily and quickly modulated via light, providing a versatile system for building platforms to control cellular events and design intelligent drug delivery systems for cell repair.

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Formation of giant plasma membrane vesicles for biological and medical applications: a review

Abstract: Plasma membrane vesicles (PMVs) are micron-sized biomembrane vesicles that are isolated directly from living cells. They retain the lipid and protein complexity of the plasma membrane of the parent cell...

Cited by 4 publications

References 68 publications

Photoswitchable Endocytosis of Biomolecular Condensates in Giant Vesicles

Photoswitchable Endocytosis of Biomolecular Condensates in Giant Vesicles

Influence of the glycocalyx on the size and mechanical properties of plasma membrane-derived vesicles

Photoswitchable endocytosis of biomolecular condensates in giant vesicles

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