Formation of Hydrogen Peroxide by a Polyvinyl Alcohol Degrading Enzyme

Watanabe, Yoshinori; Morita, Makoto; Hamada, Nobutake; Tsujisaka, Yoshio

doi:10.1080/00021369.1975.10861980

Cited by 22 publications

(24 citation statements)

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“…are commonly researched species that can produce a variety of enzymes that degrade PVA [6,10,19,24,25,28]. The PVA biodegradation process is generally divided into two steps: (1) adjacent hydroxyl groups are oxidized to form a b-diketone structure by PQQ-dependent PVA dehydrogenase (PVA-DH) [6,8,21] or secondary alcohol oxidase [5,11,19,24,27]; (2) the b-diketone is further hydrolyzed to a methyl ketone and a carboxylic acid by b-diketone hydrolase [18] and oxidized PVA hydrolase (OPH). OPH has been found to exhibit activity only towards oxidized PVA, showing no activity towards monoketones and diketones [12,22].…”

Section: Introductionmentioning

confidence: 99%

Expression and fermentation optimization of oxidized polyvinyl alcohol hydrolase in E. coli

Yang

Zhang

Liu

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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Oxidized polyvinyl alcohol (PVA) hydrolase (OPH) is a key enzyme in the degradation of PVA, suggesting that OPH has a great potential for application in textile desizing processes. In this study, the OPH gene from Sphingopyxis sp. 113P3 was modified, by artificial synthesis, for overexpression in Escherichia coli. The OPH gene, lacking the sequence encoding the original signal peptide, was inserted into pET-20b (+) expression vector, which was then used to transform E. coli BL21 (DE3). OPH expression was detected in culture medium in which the transformed E. coli BL21 (DE3) was grown. Nutritional and environmental conditions were investigated for improved production of OPH protein by the recombinant strain. The highest OPH activity measured was 47.54 U/mL and was reached after 84 h under optimal fermentation conditions; this level is 2.64-fold higher that obtained under sub-optimal conditions. The productivity of recombinant OPH reached 565.95 U/L/h. The effect of glycine on the secretion of recombinant OPH was examined by adding glycine to the culture medium to a final concentration of 200 mM. This concentration of glycine reduced the fermentation time by 24 h and increased the productivity of recombinant OPH to 733.17 U/L/h. Our results suggest that the recombinant strain reported here has great potential for use in industrial applications.

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Section: Introductionmentioning

confidence: 99%

Expression and fermentation optimization of oxidized polyvinyl alcohol hydrolase in E. coli

Yang

Zhang

Liu

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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“…The microbial degradation of PVA has been studied, as well as its enzymatic degradation by secondary alcohol peroxidases isolated from soil bacteria of the Pseudomonas strain. [9][10][11] The initial biodegradation step involves the enzymatic oxidation of the secondary alcohol groups in PVA to ketone groups. Hydrolysis of the ketone groups results in chain cleavage.…”

Section: Introductionmentioning

confidence: 99%

Preparation of resistant starch/poly(vinyl alcohol) blend films with added plasticizer and crosslinking agents

Yoon

Chough

Park

2007

J of Applied Polymer Sci

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We report on the physical properties of films synthesized with native corn starch (NCS) and resistant starch (RS4) prepared with NCS. NCS and RS4/poly(-vinyl alcohol) (PVA) blend films were synthesized with a mixing process and casting method. Glycerol (GL) and citric acid (CA) were used as additives. Glutaraldehyde (GLU) was used as a crosslinking agent of the films. RS4 was synthesized with sodium trimetaphosphate and sodium tripolyphosphate as a crosslinker. Then, the RS4 thus synthesized was confirmed by the pancreatin-gravimetry method, swelling power, differential scanning calorimetry, and X-ray diffraction. The tensile strength, elongation, swelling behavior, and solubility of the films were measured. The results of the measurements indicated that the RS4-added film was better than the NCS-added film. In particular, the RS4/PVA blend film with CA as an additive showed physical properties superior to those of the other films. Also, the physical properties with GLU added as a crosslinking agent to the films were investigated. With increasing GLU contents, the tensile strength increased but the elongation, swelling behavior, and solubility values of the GL-added and CA-added films decreased.

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“…Such polymers include polyvinyl alcohol (Suzuki et al 1973;Watanabe et al 1975), polyethers (Kawai 1990), aliphatic polyesters (Tokiwa and Suzuki 1977), polyurethane (Darby and Kaplan 1968), polyethylene (Albertsson et al 1987), butadiene oligomers (Tsuchii et al 1978), isoprene oligomers (Tsuchii et al 1979), styrene oligomers (Tsuchii et al 1977), and polyamide (Singh and V6zina 1972).…”

Section: Introductionmentioning

confidence: 99%

Bacterial degradation of acrylic oligomers and polymers

Kawai

1993

Appl Microbiol Biotechnol

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Three bacterial strains that assimilate acrylic trimer as a carbon and energy source were isolated from activated sludge and soil samples and were tentatively identified as Microbacterium sp. II-7-12, Xanthomonas maltophilia W1 and Acinetobacter genospecies 11 W2. They could assimilate acrylic monomer, dimer and trimer, but not polymers. Trimer, 0.2°70, was completely consumed in 3 days. The culture filtrate became alkaline during bacterial growth. From the values of biological 02 consumption versus theoretical 02 consumption towards oligomers and polymers, biodegradation of acrylic polymers by trimer-utilizing bacteria was suggested. The resting ceils of three bacteria grown on trimer degraded acrylic polymers (average relative molecular mass of 1000-4500) at a concentration of 100ppm (0.01%). The biodegradation rate of acrylic polymer by resting cells was calculated to be approximately 1/120 of that of acrylic trimer. Acyl-CoA synthetase activities towards oligomeric or polymeric acrylates were found with cell-free extracts of the three bacteria.

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Formation of Hydrogen Peroxide by a Polyvinyl Alcohol Degrading Enzyme

Cited by 22 publications

References 1 publication

Expression and fermentation optimization of oxidized polyvinyl alcohol hydrolase in E. coli

Expression and fermentation optimization of oxidized polyvinyl alcohol hydrolase in E. coli

Preparation of resistant starch/poly(vinyl alcohol) blend films with added plasticizer and crosslinking agents

Bacterial degradation of acrylic oligomers and polymers

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