Formation of inter‐strand cross‐linkings on DNA of guinea pig skin after application of psoralen and irradiation at 365 nm

Dall'Aqua, F; Marciani, S.; Vedaldi, Daniela; Rodighiero, G.

doi:10.1016/0014-5793(72)80617-3

Cited by 43 publications

(6 citation statements)

References 11 publications

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“…These compounds readily penetrate intact cells or viral particles, intercalate into the DNA, and, in the presence of longwavelength UV light (365 nm), covalently add to the pyrimidines of the DNA (7,27,33,38). If two pyrimidines are adjacent and on opposite strands, covalent linkages can be formed at two positions on the psoralen derivative, thus crosslinking the two strands of the double helix (8,10,11). It has been shown that the nucleosome structure is largely protected from cross-linking by 4,5',8-trimethylpsoralen (trioxsalen; Fig.…”

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confidence: 99%

Photochemical addition of the cross-linking reagent 4,5', 8-trimethylpsoralen (trioxaslen) to intracellular and viral simian virus 40 DNA-histone complexes

Hallick¹,

Yokota²,

Bartholomew³

et al. 1978

J Virol

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We demonstrated here that 4,5',8-trimethylpsoralen (trioxsalen) is a valuable probe for the structure of SV40 DNA-histone complexes. Trioxsalen readily penetrated intact cells and, in the presence of 340to 380-nm light, covalently cross-linked DNA preferentially at the sites available for micrococcal nuclease digestion. Histograms of the lengths of the regions of SV40 DNA protected from cross-linking, as visualized by electron microscopy, indicated a repeating pattern of base pairs in DNA from both infected cells and virus particles. The ability of the trioxsalen probe to act in vivo and to map the location of protected regions may provide a powerful tool for analyzing the role of nucleosomes in the structure of the virus particle and in intracellular complexes such as transcription templates and replication intermediates.

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mentioning

confidence: 99%

Photochemical addition of the cross-linking reagent 4,5', 8-trimethylpsoralen (trioxaslen) to intracellular and viral simian virus 40 DNA-histone complexes

Hallick¹,

Yokota²,

Bartholomew³

et al. 1978

J Virol

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“…Plasmid DNA, at a concentration of 10 ng/,l in TE for both ss and ds forms, was incubated with 8-MOP at 50 mM for 20 min in the dark at 4°C and irradiated with various doses, up to 5 U, of 365 nm UV radiation using a 2.5 kW Xenon lamp in a Schoeffel housing with a Kratos 252 high intensity grating monochromator (Kratos Analytical Instruments, Ramsay, NJ). The ssDNA samples were kept at 2°C during irradiation for better binding of 8-MOP to ssDNA (19).…”

Section: Plasmid Treatmentmentioning

confidence: 99%

8-Methoxypsoralen photoinduced plasmid-chromosome recombination inSaccharomyces cerevisiaeusing a centromeric vector

1995

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The characterization of a new system to study the induction of plasmid-chromosome recombination is described. Single-stranded and double-stranded centromeric vectors bearing 8-methoxypsoralen photoinduced lesions were used to transform a wild-type yeast strain bearing the leu2-3,112 marker. Using the SSCP methodology and DNA sequencing, it was demonstrated that repair of the lesions in plasmid DNA was mainly due to conversion of the chromosomal allele to the plasmid DNA.

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“…The approach we have taken is to photoreact the cells with derivatives of furocoumarins (psoralens), a class of compounds which readily penetrate intact cells or virus particles, intercalate into the DNA, and in the presence of long-wavelength UV light (365 nm), covalently add to the pyrimidines of the DNA (13)(14)(15)(16). If two pyrimidines are adjacent and on opposite strands, the two DNA strands can be covalently cross-linked (17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

Photoaddition of trimethylpsoralen as a probe for the intracellular organization of Eacherichia coli DNA

Hallick¹,

Hanson²,

Cacciapuoti³

et al. 1980

Nucl Acids Res

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It has been previously demonstrated that photoreaction with 4,5',8-tri-methylpsoralen (trioxsalen) can be used as a probe for the in vivo structure of eucaryotic chromatin. We have used this probe to analyze the organization of intracellular Escherichia coli DNA. In contrast to eucaryotic DNA, bacterial DNA within the intact cell is not protected from saturating doses of trioxsalen photoaddition. The final level of covalently bound trioxsalen upon saturation is identical to that found with purified DNA. In addition, the distribution of interstrand DNA cross-links formed by low doses of trioxsalen photoadducts does not exhibit the repeating pattern that has been observed with eucaryotic nucleosomes.

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Formation of inter‐strand cross‐linkings on DNA of guinea pig skin after application of psoralen and irradiation at 365 nm

Cited by 43 publications

References 11 publications

Photochemical addition of the cross-linking reagent 4,5', 8-trimethylpsoralen (trioxaslen) to intracellular and viral simian virus 40 DNA-histone complexes

Photochemical addition of the cross-linking reagent 4,5', 8-trimethylpsoralen (trioxaslen) to intracellular and viral simian virus 40 DNA-histone complexes

8-Methoxypsoralen photoinduced plasmid-chromosome recombination inSaccharomyces cerevisiaeusing a centromeric vector

Photoaddition of trimethylpsoralen as a probe for the intracellular organization of Eacherichia coli DNA

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