Formation of micronuclei and other nuclear anomalies in exfoliated nasal and oral cells: Results of a human study with workers in a power plant processing poultry litter

Wultsch, Georg; Nersesyan, Armen; Mišík, Miroslav; Kundi, Michael; Wagner, Karl‐Heinz; Szekeres, Thomas; Zakerska, Oliwia; Atefie, K; Knasmueller, Siegfried

doi:10.1016/j.ijheh.2012.03.007

Cited by 21 publications

(6 citation statements)

References 35 publications

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“…A positive correlation between MN in buccal cells and MN in whole blood lymphocytes was found in our study, but only for the E group. This observation agrees with a recent analysis showing that MN frequencies in exfoliated buccal cells correlate with those analyzed in peripheral lymphocytes and that both are valid biomarkers for increased cancer risk in humans (104,105).…”

Section: Discussionsupporting

confidence: 92%

Environmental Tobacco Smoke in Occupational Settings: Effect and Susceptibility Biomarkers in Workers From Lisbon Restaurants and Bars

Vital

Antunes

Louro

et al. 2021

Front. Public Health

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Environmental tobacco smoke (ETS) has been recognized as a major health hazard by environmental and public health authorities worldwide. In Portugal, smoke-free laws are in force for some years, banning smoking in most indoor public spaces. However, in hospitality venues such as restaurants and bars, owners can still choose between a total smoke-free policy or a partial smoking restriction with designated smoking areas, if adequate reinforced ventilation systems are implemented. Despite that, a previous study showed that workers remained continuously exposed to higher ETS pollution in Lisbon restaurants and bars where smoking was still allowed, comparatively to total smoke-free venues. This was assessed by measurements of indoor PM2.5 and urinary cotinine, a biomarkers of tobacco smoke exposure, demonstrating that partial smoking restrictions do not effectively protect workers from ETS. The aim of the present work was to characterize effect and susceptibility biomarkers in non-smokers from those hospitality venues occupationally exposed to ETS comparatively to non-exposed ones. A group of smokers was also included for comparison. The sister chromatid exchange (SCE), micronucleus (MN) and comet assays in whole peripheral blood lymphocytes (PBLs) and the micronucleus assay in exfoliated buccal cells, were used as biomarkers of genotoxicity. Furthermore, a comet assay after ex vivo challenge of leukocytes with an alkylating agent, ethyl methanesulfonate (EMS), was used to analyze the repair capacity of those cells. Genetic polymorphisms in genes associated with metabolism and DNA repair were also included. The results showed no clear association between occupational exposure to ETS and the induction of genotoxicity. Interestingly, the leukocytes from non-smoking ETS-exposed individuals displayed lower DNA damage levels in response to the ex vivo EMS challenge, in comparison to those from non-exposed workers, suggesting a possible adaptive response. The contribution of individual susceptibility to the effect biomarkers studied was unclear, deserving further investigation.

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Section: Discussionsupporting

confidence: 92%

Environmental Tobacco Smoke in Occupational Settings: Effect and Susceptibility Biomarkers in Workers From Lisbon Restaurants and Bars

Vital

Antunes

Louro

et al. 2021

Front. Public Health

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“…Exfoliated buccal cells were collected as described earlier 48 , 49 from the posterior part of the oral cavity close to the esophagus. Immediately before sampling, the participants were asked to rinse their mouth 2–3 times with tap water.…”

Section: Methodsmentioning

confidence: 99%

Investigations concerning the impact of consumption of hot beverages on acute cytotoxic and genotoxic effects in oral mucosa cells

Ernst

Setayesh

Nersesyan

et al. 2021

Sci Rep

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Consumption of very hot beverages and foods increases the incidence of oral and esophageal cancer but the mechanisms are not known and the critical temperature is not well defined. We realized a study with exfoliated cells from the oral cavity of individuals (n = 73) that live in an area in Iran which has the highest incidence of EC worldwide. Consumption of beverages at very high temperatures is a characteristic feature of this population. We analyzed biomarkers which are (i) indicative for genetic instability (micronuclei that are formed as a consequence of chromosomal damage, nuclear buds which are a consequence of gene amplifications and binucleated cells which reflect mitotic disturbances), (ii) markers that reflect cytotoxic effects (condensed chromatin, karyorrhectic, karyolitic and pyknotic cells), (iii) furthermore, we determined the number of basal cells which is indicative for the regenerative capacity of the buccal mucosa. The impact of the drinking temperature on the frequencies of these parameters was monitored with thermometers. We found no evidence for induction of genetic damage but an increase of the cytotoxic effects with the temperature was evident. This effect was paralleled by an increase of the cell division rate of the mucosa which was observed when the temperature exceeded 60 °C. Our findings indicate that cancer in the upper digestive tract in drinkers of very hot beverages is not caused by damage of the genetic material but by an increase of the cell division rate as a consequence of cytotoxic effects which take place at temperatures over 60 °C. It is known from earlier experiments with rodents that increased cell divisions lead to tumor promotion in the esophagus. Our findings provide a mechanistic explanation and indicate that increased cancer risks can be expected when the drinking temperature of beverages exceeds 60 °C.

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“…Noxious and harmful gases such as NO 2 , NO, and NH 3 are mainly produced by steelmaking, car exhausts, and chemical plants emitting, [ 17,18 ] their excessive inhalation can cause lung swelling and even death. [ 19,20 ] It is great significance to design an efficient sensors to detect these nitrogen‐based gases.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive and Selective Gas Sensor Using Heteroatom Doping Graphdiyne: A DFT Study

Chen

Weng

et al. 2021

Adv Elect Materials

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The emergence of a two‐dimensional (2D) functionalized‐graphene structure, graphdiyne (GDY), promoting non‐metallic single atoms level to tailor its gas sensing performance. Herein, pristine, non‐metallic atom (N, B) doped 2D GDY is investigated for toxic and greenhouse gases sensing (CO, CO2, CH4, HCHO, H2S, SO2, SO3, NO2, NO, and NH3). The B‐doped GDY (B‐GDY) compared with pure or N doped GDY displays gas sensitivity, especially excellent sensitivity and selectivity toward NO, NO2, and NH3. Additionally, a humid environment has demonstrated no effect on the weakening selectivity of B‐GDY for the studied gases. Meanwhile, the effect of external electric field (E‐field) on sensing was calculated, indicating that the bandgap of NO‐adsorbed system reached the lowest 0.188 eV at −0.5 V Å−1 and the highest 0.363 eV at −0.1 V Å−1. This work paves the way on designing efficient and effective gas sensor toward toxic nitrogen‐based gases on GDY.

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Formation of micronuclei and other nuclear anomalies in exfoliated nasal and oral cells: Results of a human study with workers in a power plant processing poultry litter

Cited by 21 publications

References 35 publications

Environmental Tobacco Smoke in Occupational Settings: Effect and Susceptibility Biomarkers in Workers From Lisbon Restaurants and Bars

Environmental Tobacco Smoke in Occupational Settings: Effect and Susceptibility Biomarkers in Workers From Lisbon Restaurants and Bars

Investigations concerning the impact of consumption of hot beverages on acute cytotoxic and genotoxic effects in oral mucosa cells

Highly Sensitive and Selective Gas Sensor Using Heteroatom Doping Graphdiyne: A DFT Study

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