Formation of new lymphatic vessels in glioma: An immunohistochemical analysis

Meng, Fanwei; Liu, Fusheng; Liu, Wenhui; Li, Li; Jie, Linlin

doi:10.1111/neup.12625

Cited by 9 publications

(11 citation statements)

References 33 publications

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“…The role of PROX1 in lymphangiogenesis was previously shown in our previous studies using a sciatic nerve injury mouse model and mouse glioma model 14 , 22 . Our findings show the association between PROX1 expression and new lymphatic cell formation as well as the correlation between new lymphatic cell formation and damaged tissue clearance.…”

Section: Discussionsupporting

confidence: 58%

“…The injury site could be detected in all 10 tissue slices for each timepoint, but the area of the injury site varied with the timepoint and depth of the slice. The 4 μm thick brain tissue sections were subjected to hematoxylin and eosin (H&E) staining according to a previous study 22 to observe tissue morphological changes, and LYVE-1 and PROX1 immunohistochemical staining and LYVE-1/CD34, LYVE-1/PROX1 immunofluorescence double staining were also performed. The average integral optical density (IOD) of LYVE-1- and PROX1-positive cells/vessels was measured using an optical microscope (DP-72; Olympus, Tokyo, Japan) at 400 × magnification.…”

Section: Methodsmentioning

confidence: 99%

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New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury

Meng

Chen

et al. 2021

Sci Rep

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We characterized the tissue repair response after penetrating traumatic brain injury (pTBI) in this study. Seventy specific pathogen-free Kunming mice were randomly divided into the following groups: normal control, 1, 3, 7, 15, 21, and 30 days after pTBI. Hematoxylin and eosin (H&E) staining, immunohistochemistry, and immunofluorescence were performed to examine and monitor brain tissue morphology, and the distribution and expression of lymphatic-specific markers lymphatic vessel endothelial receptor-1 (LYVE-1), hematopoietic precursor cluster of differentiation 34 (CD34) antigen, and Prospero-related homeobox-1 (PROX1) protein. H&E staining revealed that damaged and necrotic tissues observed on day 1 at and around the injury site disappeared on day 7, and there was gradual shrinkage and disappearance of the lesion on day 30, suggesting a clearance mechanism. We explored the possibility of lymphangiogenesis causing this clearance as part of the post-injury response. Notably, expression of lymphangiogenesis markers LYVE-1, CD34, and PROX1 was detected in damaged mouse brain tissue but not in normal tissue. Moreover, new lymphatic cells and colocalization of LYVE-1/CD34 and LYVE-1/PROX1 were also observed. Our findings of the formation of new lymphatic cells following pTBI provide preliminary insights into a post-injury clearance mechanism in the brain. Although we showed that lymphatic cells are implicated in brain tissue repair, further research is required to clarify the origin of these cells.

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Section: Discussionsupporting

confidence: 58%

Section: Methodsmentioning

confidence: 99%

New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury

Meng

Chen

et al. 2021

Sci Rep

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“…Accordingly, high expression of Prox-1 mRNA was found in: neuroblastoma, glioma, small cell lung carcinoma, colon cancer, small intestine adenocarcinoma, liver carcinoma and rhabdomyosarcoma [7]. The expression of other lymphangiogenesis markers was detected by J i a n g et al in primary and recurrent glioma tumours [22]. Consequently, its functions in different types of tumours depends on cell type [39].…”

Section: Prox-1mentioning

confidence: 96%

“…Even if, Prox-1 was highly distributed in endothelial cells of blood vessels and capillaries within glioma tissues, the glioma vasculature itself does not express Prox-1 pro-tein [22]. Tumour vessels in malignant gliomas have a lymphatic-like phenotype.…”

Section: Prox-1mentioning

confidence: 99%

The Role of Lymphatic Marker Prox-1 in Relation to Brain Tumours

Teleky

Király

2021

Folia Veterinaria

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The homeobox gene, Prox-1 is a transcription factor essential for lymphatic development (lymphangiogenesis) during embryogenesis. It also performs different functions in various tissues such as: retina, lens, liver, pancreas and the central nervous system. Intense expression of Prox-1 has been demonstrated in the developing spinal cord and brain. In adulthood its expression continues in the hippocampus and cerebellum. In adult tissues the process of lymphatic vasculature formation is accompanied under certain pathological conditions such as inflammation, tissue repair and tumour growth. Prox-1 expression is typical for lymphatic vessels; thus it belongs to one of the most specific and widely used mammalian lymphatic endothelial marker in the detection of lymphangiogenesis and lymphatic vessel invasion in oncogenesis. It has been shown that Prox-1 is involved in cancer development and progression. It’s tumour suppressive and oncogenic properties are proven in several human cancers, including brain tumours. Among all body cancers the brain tumours represent the most feared tumours with very limited treatment options and a poor diagnosis. The aim of this paper was to show the current knowledge of the gene Prox-1 with an emphasis on brain tumours, especially in gliomas.

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“…Hypoxia-inducible factor-1a (HIF-1a) is activated in the hypoxic tumor microenvironment and promotes the transcription of genes associated with tumor invasion, proliferation and radioresistance (9)(10)(11)(12)(13). The tumor cells often develop resistance to hypoxia via autophagy, a lysosome-dependent mechanism of degrading damaged proteins and organelles that ensures nutrient recycling, protein synthesis, cellular homeostasis and survival during stress (14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

Role of Borneol Induced Autophagy in Enhancing Radiosensitivity of Malignant Glioma

Liang

Sun

et al. 2021

Front. Oncol.

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Glioma is the common primary craniocerebral malignancy with unfavorable prognosis. It is currently treated by surgical resection supplemented by radiotherapy, although the resistance of glioma cells to radiation limits the therapeutic outcomes. The aim of the present study was to determine the potential radiosensitizing effects of borneol and the underlying mechanisms. We found that borneol administration along with radiotherapy significantly inhibited the growth of primary glioma cells in vitro and in vivo. Furthermore, borneol markedly increased the number of autophagosomes in the glioma cells, which coincided with increased expression of beclin-1 and LC3. And the combination of borneol and radiation exposure significantly decreased the expression levels of HIF-1α, mTORC1 and eIF4E. In addition, silencing mTORC1 and eIF4E upregulated Beclin-1 and LC3 and decreased the expression of HIF-1α, thereby inhibiting tumor cell proliferation. Our findings suggest that borneol sensitizes glioma cells to radiation by inducing autophagy via inhibition of the mTORC1/eIF4E/HIF-1α regulatory axis.

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Formation of new lymphatic vessels in glioma: An immunohistochemical analysis

Cited by 9 publications

References 33 publications

New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury

New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury

The Role of Lymphatic Marker Prox-1 in Relation to Brain Tumours

Role of Borneol Induced Autophagy in Enhancing Radiosensitivity of Malignant Glioma

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