Formation of photoproducts and cytotoxicity of bilirubin irradiated with turquoise and blue phototherapy light

Roll, Ellen; Christensen, Terje

doi:10.1080/08035250510032655

Cited by 22 publications

(13 citation statements)

References 24 publications

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“…In this study, the proportion of the apoptotic cells in the epithelium of the small intestine was 1.7% in the control group, and grew to 3.1 and 6.2% in the LED group and the conventional group, respectively. Phototherapy increases apoptosis due to cell damage depending on wavelength [11,12]. The two lamps, a turquoise (green) fluorescent phototherapy lamp (490 nm) and a conventional blue phototherapy lamp (450 nm), were similar in the formation of therapeutically relevant photoproducts, but the blue lamp showed potential in forming more severe cellular damage by the difference of wavelength [12].…”

Section: Discussionmentioning

confidence: 99%

“…Phototherapy increases apoptosis due to cell damage depending on wavelength [11,12]. The two lamps, a turquoise (green) fluorescent phototherapy lamp (490 nm) and a conventional blue phototherapy lamp (450 nm), were similar in the formation of therapeutically relevant photoproducts, but the blue lamp showed potential in forming more severe cellular damage by the difference of wavelength [12]. Bilirubin is a yellow pigment, and blue light at approximately 450 nm is the one that is most likely to be absorbed [14,19].…”

Section: Discussionmentioning

confidence: 99%

“…Phototherapy may cause apoptosis in the small intestine due to cell damage in vivo as observed in vitro [11,12].…”

Section: Introductionmentioning

confidence: 98%

“…Roll and Christensen measured apoptotic or necrotic cells induced by phototherapy using a mouse lymphoma cell line, and concluded that the emitted light increased apoptosis and necrosis due to photo-oxidation and cell damage in vitro [11,12].…”

Section: Introductionmentioning

confidence: 99%

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Apoptosis in the small intestine of neonatal rat using blue light-emitting diode devices and conventional halogen-quartz devices in phototherapy

et al. 2008

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Phototherapy is the most frequently used treatment for the neonatal jaundice. However, recent papers report that phototherapy increased apoptosis in peripheral mononuclear leukocytes in vivo and in mouse lymphoma cell line in vitro. We have investigated the cytotoxicity of phototherapy on the small intestine of neonatal rat using conventional halogen-quartz device (conventional device) and blue light-emitting device (LED device) by measuring apoptotic cells. Four-day-old male Wistar rats were divided into three groups as follows: group 1, exposure to conventional device for 72 h; group 2, exposure to LED device for 72 h; and group 3, control (without phototherapy). After light exposure, the small intestine was examined for apoptosis. Apoptotic cells were detected by the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) assay, by immunohistochemistry for caspase-3 and by transmission electron microscopy. The proportion of positive cells by the TUNEL method in the epithelium of the small intestine was 6.2, 3.1 and 1.7% in the conventional device group, the LED device group and the control group, respectively. The apoptotic cells of the conventional device group is significantly higher than the LED device group (P < 0.01) and that of the LED device group was higher than that of the control group (P < 0.05). We suspected that phototherapy induced apoptosis in neonatal small intestine and the conventional device introduces more apoptosis than the LED device.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Phototherapy may cause apoptosis in the small intestine due to cell damage in vivo as observed in vitro [11,12].…”

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Apoptosis in the small intestine of neonatal rat using blue light-emitting diode devices and conventional halogen-quartz devices in phototherapy

et al. 2008

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“…Although phototherapy has some known adverse effects including skin rash, diarrhea, dehydration, and oxidative stress, no other method with fewer adverse effects that can be used conveniently has yet been developed for the treatment of hyperbilirubinemia (1,2). In addition to the above-mentioned adverse effects, it has been shown that phototherapy might also have adverse effects on chromosomes in some animal and clinical studies (3)(4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

The effect of phototherapy on sister chromatid exchange with different light density in newborn hyperbilirubinemia

Kanmaz¹,

Okur²,

Díllí³

et al. 2018

Turk Pediatri Ars

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Aim: Concerns of possible genotoxic effects of hyperbilirubinemia and phototherapy were raised from experimental and observational studies in neonates. The purpose of this study was to assess the effect of hyperbilirubinemia and phototherapy with three different methods on DNA damage by investigating sister chromatid exchange frequency. Material and Methods: Patients whose gestational ages were >37 weeks and bilirubin levels above phototherapy limits were enrolled into three groups and each group was planned to receive 25 babies. Group 1 received enhanced light-emitting diode phototherapy, group 2 had light-emitting diode phototherapy, and group 3 received conventional phototherapy. Infants with hyperbilirubinemia but did not require phototherapy comprised the control group, which was subdivided into two groups regarding bilirubin levels (10mg/dL). Blood samples were collected before and after phototherapy for sister chromatid exchange frequency and samples were examined by a biologist who was blinded to the study groups. Results: The mean pre-treatment sister chromatid exchange frequency was 1.41±0.34/cell, post-treatment 2.65±0.68/cell, and 1.61±0.61/ cell for the control group (p<0.05). A statistically significant increase in sister chromatid exchange frequency after phototherapy was observed in all three intervention groups (p values: 0.01, 0.01, and 0.008, respectively). When the treatment groups were compared with each other in terms of irradiance, no significant difference was found (p=0.08). Conclusions: Phototherapy causes an increase in the frequency of sister chromatid exchange regardless of the irradiance. Phototherapy could have some genotoxic adverse effects on chromosomes; however, further investigations are warranted to enlighten as to whether these effects are permanent or clinically important.

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Chinese herbal medicines for the treatment of neonatal jaundice

Han

Guo

et al. 2010

Cochrane Database of Systematic Reviews

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Formation of photoproducts and cytotoxicity of bilirubin irradiated with turquoise and blue phototherapy light

Abstract: The two lamps were similar in the formation of therapeutically relevant photoproducts, but the blue lamp showed potential in forming more photo-oxidation products and in causing more severe cellular damage in the presence of bilirubin.

Cited by 22 publications

References 24 publications

Apoptosis in the small intestine of neonatal rat using blue light-emitting diode devices and conventional halogen-quartz devices in phototherapy

Apoptosis in the small intestine of neonatal rat using blue light-emitting diode devices and conventional halogen-quartz devices in phototherapy

The effect of phototherapy on sister chromatid exchange with different light density in newborn hyperbilirubinemia

Chinese herbal medicines for the treatment of neonatal jaundice

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