Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles

Vamanu, Carmen I.

doi:10.2147/ijn.s1796

Cited by 28 publications

(18 citation statements)

References 39 publications

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“…This phenomenon is equivalent to the drop in the TSI value (for example, for BSA at pH 3-25.9), the backscatter level increase (for BSA at pH 3 from 10 to 18 % in the last minutes of the measurement) and the decrease in transmission level (for BSA at pH 3 from 25 to 12 % in the last minutes of the measurement). A similar serum protein effect was observed during the study of inorganic compounds, i.e., TiO 2 (Allouni et al 2009;Meißner et al 2009;Vamanu et al 2008), Fe 2 O 3 and ZnO (Wells et al 2011), WC (Meißner et al 2009), and fullerene (Deguchi et al 2007). On the other hand, Flynn et al (2012) claimed that The result analysis showed that the isoelectric point (pH iep ) of chromium(III) oxide is approximately six.…”

Section: Resultssupporting

confidence: 73%

Investigation of removal possibilities of chromium(III) oxide from water solution in the presence of albumins

Szewczuk-Karpisz

Wiśniewska

2014

Int. J. Environ. Sci. Technol.

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System destabilization is a highly desirable phenomenon during colloidal impurity removal from sewages and wastewaters. Therefore, in this study, the stability of chromium(III) oxide suspension in the absence and presence of albumins [bovine serum albumin (BSA), ovalbumin (OVA), human serum albumin (HSA)] was investigated. Based on the analysis of experimental results, i.e., measurements of adsorption amount, electrokinetic potential, and metal oxide surface charge density as well as system stability, the mechanism of the suspension stabilization/destabilization was proposed. The examined system without the albumins is relatively stable at pH 3, 4.6, and 9 (TSI = 34.8, 36.6, 34.22, respectively), which is associated with the electrostatic stabilization phenomenon. In turn, the least stable is the suspension at pH 7.6 (TSI = 55.43). This is the result of the adsorbent zero surface charge. Regardless of solution pH, the BSA, OVA, or HSA adsorption causes an increase in the system stability (17.55 \ TSI \ 30). Probably, the steric stabilization is involved in this phenomenon, which results from the mutual repulsion of the adsorption layers formed on the solid surface. Thus, it can be concluded that the albumin presence in the industrial wastewaters impedes the chromium(III) oxide removal.

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Section: Resultssupporting

confidence: 73%

Investigation of removal possibilities of chromium(III) oxide from water solution in the presence of albumins

Szewczuk-Karpisz

Wiśniewska

2014

Int. J. Environ. Sci. Technol.

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“…The low zeta potentials (absolute values) following protein adsorption (Table 2) support the hypothesis that the stabilizing effect of proteins is not related to electrostatic repulsion, but rather to steric repulsion. [37][38][39] Some agglomeration over time is however inevitable, even in the presence of proteins. In the presence of Glbs and Fib, the polydispersity index was high, which means a broad size distribution.…”

Section: Protein Adsorption Onto Tio 2 Npsmentioning

confidence: 99%

The effect of blood protein adsorption on cellular uptake of anatase TiO2 nanoparticles

Allouni¹,

Gjerdet²,

Cimpan³

et al. 2015

IJN

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Protein adsorption onto nanoparticles (NPs) in biological fluids has emerged as an important factor when testing biological responses to NPs, as this may influence both uptake and subsequent toxicity. The aim of the present study was to quantify the adsorption of proteins onto TiO 2 NPs and to test the influence on cellular uptake. The surface composition of the particles was characterized by thermal analysis and by X-ray photoelectron spectroscopy. The adsorption of three blood proteins, ie, human serum albumin (HSA), γ-globulins (Glbs), and fibrinogen (Fib), onto three types of anatase NPs of different sizes was quantified for each protein. The concentration of the adsorbed protein was measured by ultraviolet-visible spectrophotometry using the Bradford method. The degree of cellular uptake was quantified by inductivity coupled plasma mass spectroscopy, and visualized by an ultra-high resolution imaging system. The proteins were adsorbed onto all of the anatase NPs. The quantity adsorbed increased with time and was higher for the smaller particles. Fib and Glbs showed the highest affinity to TiO 2 NPs, while the lowest was seen for HSA. The adsorption of proteins affected the surface charge and the hydrodynamic diameter of the NPs in cell culture medium. The degree of particle uptake was highest in protein-free medium and in the presence HSA, followed by culture medium supplemented with Glbs, and lowest in the presence of Fib. The results indicate that the uptake of anatase NPs by fibroblasts is influenced by the identity of the adsorbed protein.

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“…However, in some cases, the greyish color of a titanium implant may be perceived through the peri-implant mucosa causing some aesthetic drawbacks [4]. Furthermore, in rare cases, metals (including titanium) may induce sensitization or allergic reactions [5,6]. Finally, some patients also ask for completely metal-free dental reconstructions.…”

Section: Introductionmentioning

confidence: 99%

A new testing protocol for zirconia dental implants

et al. 2015

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Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles

Cited by 28 publications

References 39 publications

Investigation of removal possibilities of chromium(III) oxide from water solution in the presence of albumins

Investigation of removal possibilities of chromium(III) oxide from water solution in the presence of albumins

The effect of blood protein adsorption on cellular uptake of anatase TiO2 nanoparticles

A new testing protocol for zirconia dental implants

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