Formation of Solid-Supported Lipid Bilayers:  An Integrated View

Richter, Ralf P.; Bérat, Rémi; Brisson, Alain

doi:10.1021/la052687c

Cited by 1,022 publications

(1,212 citation statements)

References 94 publications

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“…Furthermore, the overlapping degree of ∆f/n for the different harmonics increases, indicating an increase in rigidity. This behaviour suggests rupture and eventual removal of liposomes from the crystal surface, being the final values of ∆f/n and ∆D consistent with the presence of supported bilayer patches [29,30]. Experimental results reported in the literature confirm that at high concentrations, TTC originates significant changes in the membrane structure: it may form TTC-lipid mixed micelles or dimers and ultimately dissolve the membranes, due to its surfactant properties [13].…”

Section: Qcm-d Experimentsmentioning

confidence: 64%

Effect of tetracaine on DMPC and DMPC+cholesterol biomembrane models: Liposomes and monolayers

Serro

Galante

Kozica

et al. 2014

Colloids and Surfaces B: Biointerfaces

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Different types of lipid bilayers/monolayers have been used to simulate the cellular membranes in the investigation of the interactions between drugs and cells. However, to our knowledge, very few studies focused on the influence of the chosen membrane model upon the obtained results. The main objective of this work is to understand how do the nature and immobilization state of the biomembrane models influence the effect of the local anaesthetic tetracaine (TTC) upon the lipid membranes. The interaction of TTC with different biomembrane models of dimyristoylphosphatidylcholine (DMPC) with and without cholesterol (CHOL) was investigated through several techniques. A quartz crystal microbalance with dissipation (QCM-D) was used to study the effect on immobilized liposomes, while phosphorus nuclear magnetic resonance ( 31 P-NMR) and differential scanning calorimetry (DSC) were applied to liposomes in suspension. The effect of TTC on Langmuir monolayers of lipids was also investigated through surface pressure-area measurements at the air-water interface. The general conclusion was that TTC has a fluidizing effect on the lipid membranes and, above certain concentrations, induced membrane swelling or even solubilization. However, different models led to variable responses to the TTC action. The intensity of the disordering effect caused by TTC increased in the following order: supported liposomes < liposomes in solution < Langmuir monolayers. This means that extrapolation of the results obtain in in vitro studies of the lipid/anaesthetic interactions to in vivo conditions should be done carefully.

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Section: Qcm-d Experimentsmentioning

confidence: 64%

Effect of tetracaine on DMPC and DMPC+cholesterol biomembrane models: Liposomes and monolayers

Serro

Galante

Kozica

et al. 2014

Colloids and Surfaces B: Biointerfaces

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“…The protein-modified liposomes were then introduced to the pretreated slides in a perfusion chamber. After incubating for 2 h at room temperature, liposomes fused on the substrate, and spontaneously formed a supported lipid bilayer (30,31). The bilayer was rinsed thoroughly with water to remove excess liposomes and protein, and then exchanged into HEPES/CaCl 2 buffer at the desired Ca 2þ concentration (32,33).…”

Section: Sample Preparationmentioning

confidence: 99%

Cadherin Diffusion in Supported Lipid Bilayers Exhibits Calcium-Dependent Dynamic Heterogeneity

Cai

Shashikanth

Leckband

et al. 2016

Biophysical Journal

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Ca 2þ ions are critical to cadherin ectodomain rigidity, which is required for the activation of adhesive functions. Therefore, changes in Ca 2þ concentration, both in vivo and in vitro, can affect cadherin conformation and function. We employed single-molecule tracking to measure the diffusion of cadherin ectodomains tethered to supported lipid bilayers at varying Ca 2þ concentrations. At a relatively high Ca 2þ concentration of 2 mM, cadherin molecules exhibited a fast diffusion coefficient that was identical to that of individual lipid molecules in the bilayer (D fast z 3 mm 2 /s). At lower Ca 2þ concentrations, where cadherin molecules were less rigid, the ensemble-average cadherin diffusion coefficient was systematically smaller. Individual cadherin trajectories were temporally heterogeneous, exhibiting alternating periods of fast and slow diffusion; the periods of slow diffusion (D slow z 0.1 mm 2 /s) were more prevalent at lower Ca 2þ concentration. These observations suggested that more flexible cadherin ectodomains at lower Ca 2þ concentration alternated between upright and lying-down conformations, where the latter interacted with more lipid molecules and experienced greater viscous drag.

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“…When small unilamellar vesicles, typically with a diameter of B100 nm, are incubated with hydrophilic solid supports, the vesicles are adsorbed on the surface and deformed by interaction with the solid substrate. 15 The support-induced stress is further enhanced by adsorption of the vesicles in their vicinity. The adsorbed liposomes rupture and form a continuous lipid bilayer on the solid support at a certain surface density of vesicles that corresponds to a critical vesicular coverage.…”

Section: Formation Of Lipid-nanostructure Hybrids and Their Structurementioning

confidence: 99%

Lipid-nanostructure hybrids and their applications in nanobiotechnology

Lee

Nam

2013

NPG Asia Mater

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Cell membranes contain a variety of lipids and functional proteins that offer active platforms that organize the membrane components into functional assemblies and perform biologically important reactions. The dynamic and complex nature of the membranes makes them attractive materials, but at the same time, researchers report substantial experimental uncertainty in controlling the membrane reactions and extracting valuable information. The fascinating new structures and properties of nanomaterials could be utilized in addressing aforementioned issues, and the design and synthesis of lipid-nanostructure hybrids could be beneficial to the research areas in lipid-membrane biotechnology and nanobiotechnology. These hybrid structures possess dimensions that are comparable to that of biological molecules and structures and physicochemical properties that arise from both lipids and nanomaterials. Therefore, lipid-nanostructure hybrids offer additional options for control of the synthesized structures, provide new insight in understanding nanostructures and biological systems and allow the mimicking of functional subcellular membrane components and monitoring of the membrane-associated reactions in a highly sensitive and controllable manner. In this review, we present recent advances in the synthesis of various lipid-nanostructure hybrids and the application of these structures in biotechnology and nanotechnology. We further describe the scientific and practical applications of lipid-nanostructure hybrids for detecting membrane-targeting molecules, interfacing nanostructures with live cells and creating membrane-mimicking platforms to investigate various intercellular processes.

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Formation of Solid-Supported Lipid Bilayers: An Integrated View

Cited by 1,022 publications

References 94 publications

Effect of tetracaine on DMPC and DMPC+cholesterol biomembrane models: Liposomes and monolayers

Effect of tetracaine on DMPC and DMPC+cholesterol biomembrane models: Liposomes and monolayers

Cadherin Diffusion in Supported Lipid Bilayers Exhibits Calcium-Dependent Dynamic Heterogeneity

Lipid-nanostructure hybrids and their applications in nanobiotechnology

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