Formation, physicochemical properties, and comparison of heat- and enzyme-induced whey protein-gelatin composite hydrogels

Yan, Jun; Li, Siqi; Chen, Guipan; Ma, Che; McClements, David Julian; Liu, Xuebo; Liu, Fuguo

doi:10.1016/j.foodhyd.2022.108384

Cited by 34 publications

(10 citation statements)

References 53 publications

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“…Thermally induced crosslinking involves heating the hydrogel solution to a temperature above the polymer melting point or glass transition temperature [68] . The heat causes the polymer chains to become more mobile and enables them to form non‐covalent interactions with each other.…”

Section: Preparation Methodsmentioning

confidence: 99%

“…Thermally induced crosslinking involves heating the hydrogel solution to a temperature above the polymer melting point or glass transition temperature. [68] The heat causes the polymer chains to become more mobile and enables them to form non-covalent interactions with each F I G U R E 3 Assorted chemical methods for the hydrogel preparations. (a) Physical adsorption preparation of cellulose/GO/TiO 2 composite hydrogel.…”

Section: Thermally Induced Crosslinkingmentioning

confidence: 99%

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Hydrogel wound dressings for diabetic foot ulcer treatment: Status‐quo, challenges, and future perspectives

Ko¹,

Liao

2023

BMEMat

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Diabetic foot ulcers (DFU) are a common and often debilitating complication of diabetes that can result in lower limb amputations if left untreated. Hydrogel dressings are three‐dimensional networks of hydrophilic polymers that can absorb and retain large amounts of water, and have been shown to possess excellent biocompatibility, low toxicity, and excellent fluid handling properties. In addition, hydrogels create a moist wound environment that promotes wound healing by supporting cell proliferation, migration, and angiogenesis. Hydrogels, therefore, have emerged as promising wound dressings for promoting DFU healing. In this review, we attempt to chart the landscape of the emerging field of hydrogel as wound dressing for DFU treatment. We will explicitly review the assorted preparation methods for DFU hydrogels as well as a detailed discussion of various types of hydrogels deployed for DFU study. We also crystallize key findings, identify remaining challenges, and present an outlook on the future development of this enticing field.

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Section: Preparation Methodsmentioning

confidence: 99%

Section: Thermally Induced Crosslinkingmentioning

confidence: 99%

Hydrogel wound dressings for diabetic foot ulcer treatment: Status‐quo, challenges, and future perspectives

Ko¹,

Liao

2023

BMEMat

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“…35−38 The ultrasonication used for homogenization of emulsions promotes not only the emulsion formation but also the gelification of the WPI protein shell due to the WPI denaturation and the formation of a threedimensional hydrogel network. 39 The ability of WPI to attach to the tissue mucosa 40 is used in pharmaceuticals to create mucoadhesive drug delivery forms, for example, Loramyc for the treatment of oral candidiasis. 41 Therefore, emulsions stabilized on mucoadhesive WPI are of great practical importance for intravesical delivery.…”

Section: Introductionmentioning

confidence: 99%

“…Particular attention is paid to emulsion systems intended for intravesical drug delivery due to their ability to immobilize both hydrophobic and hydrophilic molecules. − Mucoadhesive emulsions are of increasing interest given the combination of properties which are beneficial for intravesical drug delivery. − In our study, mucoadhesive emulsion microgels were prepared on the basis of a naturally derived component, whey protein isolate (WPI), which is a byproduct of whey in cheese production . WPI is being extensively studied as a hydrogel material for biomedical applications. − Moreover, WPI can be used as an emulsifier and stabilizer to produce oil-in-water emulsions. − The ultrasonication used for homogenization of emulsions promotes not only the emulsion formation but also the gelification of the WPI protein shell due to the WPI denaturation and the formation of a three-dimensional hydrogel network . The ability of WPI to attach to the tissue mucosa is used in pharmaceuticals to create mucoadhesive drug delivery forms, for example, Loramyc for the treatment of oral candidiasis .…”

Section: Introductionmentioning

confidence: 99%

Mucoadhesive Emulsion Microgels for Intravesical Drug Delivery: Preparation, Retention at Urothelium, and Biodistribution Study

Saveleva,

Lobanov,

Gusliakova

et al. 2023

ACS Appl. Mater. Interfaces

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The intravesical instillation procedure is a proven method in modern urology for the treatment of bladder diseases. However, the low therapeutic efficiency and painfulness of the instillation procedure are significant limitations of this method. In the present study, we propose an approach to solving this problem by using microsized mucoadhesive macromolecular carriers based on whey protein isolate with the possibility of prolonged release of drugs as a drug delivery system. The optimal water-to-oil ratio (1:3) and whey protein isolate concentration (5%) were determined to obtain emulsion microgels with sufficient loading efficiency and mucoadhesive properties. The droplet diameter of emulsion microgels varies from 2.2 to 3.8 μm. The drug release kinetics from the emulsion microgels was evaluated. The release of the model dye in saline and artificial urine in vitro was observed for 96 h and reached up to 70% of loaded cargo for samples. The effect of emulsion microgels on the morphology and viability of two cell lines was observed: L929 mouse fibroblasts (normal adherent cells) and THP-1 human monocytes (cancer suspension cells). Developed emulsion microgels (5%, 1:3 and 1:5) showed sufficient mucoadhesion to a porcine bladder urothelium ex vivo. The biodistribution of emulsion microgels (5%, 1:3 and 1:5) in mice (n = 3) after intravesical (instillation) and systemic (intravenous) administration was assessed in vivo and ex vivo using nearinfrared fluorescence live imaging for real time. It was demonstrated that intravesical instillation allows approximately 10 times more efficient accumulation of emulsion microgels in the mice urinary bladder in vivo 1 h after injection compared to systemic injection. The retention of the emulsion of mucoadhesive microgels in bladders after the intravesical instillation was observed for 24 h.

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“…Conversely, if the pH of the gelatin is higher than the isoelectric point, then the surface of the gelatin molecules will be negatively charged as the functional groups become deprotonated, meaning the molecules will experience repulsion from other negatively charged chain fragments and ionic species (Figure 3). 7,[13][14][15] While advantageous, the thermoreversible nature of the gelatin network at relatively low temperatures (25-28 C) limits the application of these hydrogels, particularly for medical applications. 16 In order to improve the thermal and mechanical properties of these materials chemical crosslinking is often carried out using aldehydes, such as formaldehyde and glutaraldehyde, or by photoinitiation.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the influence of pH on the properties and morphology of gelatin hydrogels

Goudie

McCreath

Parkinson

et al. 2023

Journal of Polymer Science

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The behavior of gelatin hydrogels is influenced by the charges located on the amino acid side chains throughout the gelatin molecules. The presence and distribution of ionisable side chains influences the surface activity of gelatin and ultimately determines the material properties. Herein, we report the influence of pH on mechanical properties as studied by texture analysis supported by data from polarimetry, zeta potential, pH titrations and NMR experiments. When adjusted to more extreme pH values (pH 2 and 12), softer gelatin blocks were observed. However, at pH values close to the isoelectric point (pH 5–10), the material is firmer. This behavior is related to the helical content. At pH 2 and pH 12 the surface of the gelatin carries a net charge, positive and negative, respectively, that inhibits the formation of tight helices and lowers the physical crosslink network density. Chemical shift perturbations were observed for the acidic amino acids glutamic and aspartic acid, under acidic pH, where their peaks shifted to higher ppm. Intense amide signals were observed at acidic pH but diminished with increasing pH. This was due to an increase in the rate of chemical exchange between the solvent and peptide amide protons as the pH increases.

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Formation, physicochemical properties, and comparison of heat- and enzyme-induced whey protein-gelatin composite hydrogels

Cited by 34 publications

References 53 publications

Hydrogel wound dressings for diabetic foot ulcer treatment: Status‐quo, challenges, and future perspectives

Hydrogel wound dressings for diabetic foot ulcer treatment: Status‐quo, challenges, and future perspectives

Mucoadhesive Emulsion Microgels for Intravesical Drug Delivery: Preparation, Retention at Urothelium, and Biodistribution Study

Investigation of the influence of pH on the properties and morphology of gelatin hydrogels

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