Formulation and evaluation of chitosan films containing sparfloxacin for the treatment of periodontitis

Abstract

In the present study an attempt has been made to formulate and evaluate a sustained release periodontal film of Sparfloxacin with biodegradable, cost effective polymer Chitosan. The objective of the study was to formulate intra-pocket periodontal films, which could be easily placed into the periodontal pocket, and thus be capable of delivering therapeutic concentrations of drug. Sparfloxacin is an antibiotic, showing wide spectrum antibacterial activity against a number of periodontal pathogens. Hence Sparfloxacin is selected as model for site specific delivery, i.e., into periodontal pocket for the treatment of periodontitis. In the present investigation Chitosan films containing Sparfloxacin were prepared by solution casting method using acetic acid. The copolymers HPMC K4M, Sodium CMC and Eudragit RL 100 in the concentrations of 10%, 20% and 30% w/w of Chitosan were added into the polymeric solution. Propylene glycol was used as plasticizer. FT-IR and UV spectroscopic methods revealed no interaction between Sparfloxacin and polymers. The drug loaded films were evaluated for their thickness, weight variation, content uniformity, tensile strength, percent elongation, percentage moisture loss, surface pH, folding endurance, in-vitro drug release studies, in -vitro antibacterial activity and stability studies. Periodontal films showed initial burst release of drug on 1 st day and then the release was sustained for a period of 8 days. In -vitro antibacterial activity was carried out on staphylococcus aureus and the antibacterial activity was retained for 96 hours. In -vitro release from periodontal films was fit to kinetic models to reveal drug release kinetics.

Enhanced Antibacterial and Antioxidant Properties of Chitosan Films Blended with Gallic Acid and Incorporated with Thymol Silver Nanoparticles

“…Visual appearance. The Visual appearance C+G film and C+G/GA/T-SNPs film were observed for colour and physical form or appearance (Naik et al 2019).…”

“…The thickness of each film was measured using screw guage (thickness tester) at different position of the film and the average was calculated (Naik et al 2019).…”

“…This was termed as one folding. The cycle was repeated till the film showed rupture or breaks in centre of film or folded up to 100 times which is considered satisfactory to reveal good film properties (Naik et al 2019). The total numbers of folding were named as folding endurance value.…”

Influence of Materials Properties on Bio-Physical Features and Effectiveness of 3D-Scaffolds for Periodontal Regeneration

“…Sparfloxacin-loaded chitosan-based film was also proposed as an antimicrobial device for the treatment of periodontal diseases [ 74 ]. In this case, the film preparation was carried out using chitosan alone or in combination with various co-polymers, i.e., hydroxypropyl cellulose, carboxymethyl cellulose and Eudragit RL100.…”

PVA/Chitosan Thin Films Containing Silver Nanoparticles and Ibuprofen for the Treatment of Periodontal Disease

“…Due to its interesting properties, CS is extensively used in biomedical applications as a matrix for drug encapsulation and controlled release [ 7 , 8 ], but also as an antimicrobial agent, alone or with other natural and synthetic polymers [ 9 , 10 , 11 , 12 ]. CS is also known to have antimicrobial activity against periodontal pathogens ( Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans ) [ 13 , 14 ] and was used with good results for periodontal treatment in clinical trials [ 15 , 16 ], as well as a film matrix for the periodontal delivery of different drugs, such as tetracycline [ 17 ], ciprofloxacin and diclofenac [ 18 ], lincomycin [ 19 ], metronidazole [ 20 ], risedronate [ 21 ], metformin [ 22 ] or sparfloxacin [ 23 ].…”