Formulation and evaluation of gemcitabine-loaded solid lipid nanoparticles

Abstract

Gemcitabine-loaded solid lipid nanoparticles (SLNs) were produced by double emulsification technique using stearic acid as lipid, soy lecithin as surfactant and sodium taurocholate as cosurfactant. Prepared nanoparticles are characterized for particle size and surface morphology using scanning electron microscopy (SEM). Particle yield, entrapment efficiency and zeta potential were also determined. In-vitro release studies were performed in phosphate-buffered saline (PBS) pH 7.4 using metabolic shaker. The formulation F6 with maximum entrapment efficiency 72.42% and satisfactory in-vitro release was selected. In-vivo tissue distribution to liver, spleen, lung, heart and kidneys of optimized formulation followed by stability study under specific conditions were also determined. This investigation has shown preferential drug targeting to liver followed by spleen, lungs, kidneys and heart. Stability studies showed no significant change in the particle size followed with very slight decrease in entrapment efficiency at 25 AE 2 C/60 AE 5% RH over a period of three months.

Formulation, Characterization, in vitro Anti-Tubercular Activity and Cytotoxicity Study of Solid Lipid Nanoparticles of Isoniazid

“…Diffusion of INZ from lipid matrix also depends on the partitioning of INZ between the lipid phase and the aqueous dissolution media. The same pattern of release was also reported for gemcitabine [29].…”

<p>Transdermal permeability of triamcinolone acetonide lipid nanoparticles</p>

“…The EE of LPNs was higher than that of nanoparticles and common liposomes because the lipid material and the nanoparticle solution were incubated at a certain temperature, and the vesicles exhibited a fusion effect after coming into contact with the nanoparticles and recombining into a continuous bilayer around the nanoparticles, thereby increasing the EE of the LPNs (Figure 2; Table 2). [29][30][31] The preliminary stability evaluation of LNPs was performed by using the influencing factors test according to the guiding principle of the stability test of the preparation, which provided a basis for the subsequent optimization of the preparation process and storage conditions. The results indicated that the LNPs were sensitive to temperature.…”

Development and characterization of folic acid-conjugated chitosan nanoparticles for targeted and controlled delivery of gemcitabinein lung cancer therapeutics

“…Balb/c mice (4-5 weeks old, weight 25 ± 2 g) were procured from Liaocheng People's Hospital, Liaocheng, China. The pharmacokinetics study was conducted by following the previously reported procedure (Nandini et al 2015) with slight modifications. Four animals each were divided into three groups.…”

“…The rapid metabolism and shorter half-life of drug required higher dose and repeated dosing schedule which consequently results in higher toxicity. Thus, it is needed to design a vector which can reduce the burden of frequent dosing and higher toxicity associated with high dosages of GEM (Nandini et al 2015, Sandler et al 2000. Therefore, the novel drug delivery strategies are required to increase the bioavailability of drugs and to reduce side effects.…”