Formulation and Evaluation of in-Situ Forming Liposomes of Gliclazide

Abstract

An approach was used to enhance the solubility of BCS Class II drug, Gliclazide. The objective of this study was to formulate liposomal drug delivery system and to show the potential of Soya lecithin, tween 80 in enhancing the solubility and bioavailability of Gliclazide. Initial preparations were done by mixing the drug and carrier. Formulations were prepared by heat fusion method as it is considered as the mechanism for the enhancement of solubility and dissolution of the drug. The in-vitro releases of the different formulations were studied based on the effect of surfactant and oil including their thermodynamic stability. Formulated drug and adjuvants were characterized by spectrophotometry (UV, FTIR and photon correlation). Dissolution studies showed that F3 had the smallest particle size of 127.6 nm, with values for other formulations ranging from 176.8-248.8 nm. The cumulative percentage release for all formulations ranged from 21.20 ± 1.68% to 80.92 ± 3.82%; with F3 having the highest value of 80.92 ± 3.82%. Soya lecithin, soybean oil and tween 80 showed no significant influence on formulation's stability. These results confirm that the potential of liposomal drug delivery containing oil and surfactants as an adjuvant are expected to increase the oral bioavailability as confirmed by the increased in-vitro release.