Formulation of fermentation media from flour-rich waste streams for microbial lipid production by Lipomyces starkeyi

Tsakona, Sofia; Kopsahelis, Nikolaos; Chatzifragkou, Afroditi; Papanikolaou, Séraphim; Kookos, Ioannis K.; Koutinas, Apostolis

doi:10.1016/j.jbiotec.2014.08.011

Cited by 100 publications

(80 citation statements)

References 35 publications

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“…for 100 mg of DCW, 10 ml of 2·5 mol l −1 HCl at T = 100°C for 30 min should be added) and then the mixture of chloroform/methanol 2/1 (v/v) should be used to extract total lipids from the acidified biomass, otherwise the extraction of the microbial lipid is completely deficient (Tsakona et al . ; Tchakouteu et al . ).…”

Section: Analytical Aspectsmentioning

confidence: 99%

Lipids from yeasts and fungi: physiology, production and analytical considerations

et al. 2018

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The last years there has been a significant rise in the number of publications in the international literature that deal with the production of lipids by microbial sources (the 'single cell oils; SCOs' that are produced by the so-called 'oleaginous' micro-organisms). In the first part of the present review article, a general overview of the oleaginous micro-organisms (mostly yeasts, algae and fungi) and their potential upon the production of SCOs is presented. Thereafter, physiological and kinetic events related with the production of, mostly, yeast and fungal lipids when sugars and related substrates like polysaccharides, glycerol, etc. (the de novo lipid accumulation process) or hydrophobic substrates like oils and fats (the ex novo lipid accumulation process) were employed as microbial carbon sources, are presented and critically discussed. Considerations related with the degradation of storage lipid that had been previously accumulated inside the cells, are also presented. The interplay of the synthesis of yeast and fungal lipids with other intracellular (i.e. endopolysaccharides) or extracellular (i.e. citric acid) secondary metabolites synthesized is also presented. Finally, aspects related with the lipid extraction and lipidome analysis of the oleaginous micro-organisms are presented and critically discussed.

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Section: Analytical Aspectsmentioning

confidence: 99%

Lipids from yeasts and fungi: physiology, production and analytical considerations

et al. 2018

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“…Other examples of wastes include: palm oil mill effluent (POME), a waste from palm oil wet milling; oil waste from fast food services; wastewater and sewage sludge [106]. Lignocellulosic raw materials, after a pretreatment in order to produce fermented monomers, have also been studied for microbial oil production [52,113]. Zhao et al [104] observed that the fatty acid composition of the yeast lipid from R. toruloides, obtained by fermentation using lignocellulosic hydrolyzate as substrate, was in the range of those of various plant oils and animal tallow.…”

Section: Other Potential Feedstock: Waste Oils Non-edible Oils and Omentioning

confidence: 99%

Current status and new developments of biodiesel production using fungal lipases

Aguieiras

Cavalcanti-Oliveira

Freire

2015

Fuel

122

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“…However, very harsh pretreatment conditions can lead to SCO degradation. Different pretreatments have been evaluated separately or together, such as the addition of base [20], acid [19,25,26], and enzyme [18] and the application of sonication [27] and microwaves [18].…”

mentioning

confidence: 99%

Single Cell Oil Producing Yeasts Lipomyces starkeyi and Rhodosporidium toruloides: Selection of Extraction Strategies and Biodiesel Property Prediction

et al. 2015

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Single cell oils (SCOs) are considered potential raw material for the production of biodiesel. Rhodosporidium sp. and Lipomyces sp. are good candidates for SCO production. Lipid extractability differs according to yeast species and literature on the most suitable method for each oleaginous yeast species is scarce. This work aimed to investigate the efficiency of the most cited strategies for extracting lipids from intact and pretreated cells of Rhodosporidium toruloides and Lipomyces starkeyi. Lipid extractions were conducted using hexane or combinations of chloroform and methanol. The Folch method resulted in the highest lipid yields for both yeasts (42% for R. toruloides and 48% for L. starkeyi). Also, this method eliminates the cell pretreatment step. The Bligh and Dyer method underestimated the lipid content in the tested strains (25% for R. toruloides and 34% for L. starkeyi). Lipid extractability increased after acid pretreatment for the Pedersen, hexane, and Bligh and Dyer methods. For R. toruloides unexpected fatty acid methyl esters OPEN ACCESSEnergies 2015, 8 5041 (FAME) composition were found for some lipid extraction strategies tested. Therefore, this work provides useful information for analytical and process development aiming at biodiesel production from the SCO of these two yeast species.

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Formulation of fermentation media from flour-rich waste streams for microbial lipid production by Lipomyces starkeyi

Cited by 100 publications

References 35 publications

Lipids from yeasts and fungi: physiology, production and analytical considerations

Lipids from yeasts and fungi: physiology, production and analytical considerations

Current status and new developments of biodiesel production using fungal lipases

Single Cell Oil Producing Yeasts Lipomyces starkeyi and Rhodosporidium toruloides: Selection of Extraction Strategies and Biodiesel Property Prediction

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