Formulation optimization for the nanoparticles-in-microsphere hybrid oral delivery system using factorial design

Oral IL-10 gene delivery in a microsphere-based formulation for local transfection and therapeutic efficacy in inflammatory bowel disease

“…24,25 Briefly, 10 mg of plasmid DNA-encapsulated gelatin nanoparticles were suspended in distilled deionized water. This suspension was added to 2.0 ml of dichloromethane containing 0.5% (w/v) poly(epsilon-caprolactone) and homogenized at room temperature.…”

“…[17][18][19][20][21][22][23] In previous studies, we have described the formulation development, characterization, optimization and preliminary in vivo transfection of reporter plasmids upon oral administration of nanoparticles-in-microsphere oral system (NiMOS) formulations in animal models. 24,25 NiMOS is a biodegradable and biocompatible polymer-based microparticle system capable of plasmid DNA delivery and local transfection upon oral administration. This delivery system is specifically engineered for oral gene therapy, where the DNAencapsulated gelatin nanoparticles can be released in the intestine from poly(epsilon-caprolactone) matrix due to lipase-induced degradation.…”

Nano and Microparticulate Chitosan Based System for Formulation of Carvedilol Rapid Melt Tablet

“…Recently, a novel "nano and microparticulate" chitosan based system (NMCS) has been developed using a double emulsion like technique and evaluated for drug and gene delivery in specific regions of the gastrointestinal tract. 10,11 Spraydrying has been used to increase drug solubility and bioavailability of active substances, modified release. 12,13 Microparticles can be used for the controlled release of drugs, vaccines, antibiotics, and hormones.…”

Crystalline and amorphous carvedilol-loaded nanoemulsions: formulation optimisation using response surface methodology

“…In addition to the art of formulation, the technique of factorial design is an efficient method of indicating the relative significance of a number of variables and their interactions [24]. The response surface method, first reported by Box and Wilson [25], has been applied to dosage form design for various kinds of drugs by several other researchers [26][27][28]. Factorial design is a very efficient tool to obtain an appropriate mathematical model with minimum experiments for the optimisation of formulation design.…”