Fostering the action of versatile peroxidase as a highly efficient biocatalyst for the removal of endocrine disrupting compounds

Effect-directed analysis (EDA) is a commonly used approach for effect-based identification of endocrine disruptive chemicals in complex (environmental) mixtures. However, for routine toxicity assessment of, for example, water samples, current EDA approaches are considered time-consuming and laborious. We achieved faster EDA and identification by downscaling of sensitive cell-based hormone reporter gene assays and increasing fractionation resolution to allow testing of smaller fractions with reduced complexity. The high-resolution EDA approach is demonstrated by analysis of four environmental passive sampler extracts. Downscaling of the assays to a 384-well format allowed analysis of 64 fractions in triplicate (or 192 fractions without technical replicates) without affecting sensitivity compared to the standard 96-well format. Through a parallel exposure method, agonistic and antagonistic androgen and estrogen receptor activity could be measured in a single experiment following a single fractionation. From 16 selected candidate compounds, identified through nontargeted analysis, 13 could be confirmed chemically and 10 were found to be biologically active, of which the most potent nonsteroidal estrogens were identified as oxybenzone and piperine. The increased fractionation resolution and the higher throughput that downscaling provides allow for future application in routine high-resolution screening of large numbers of samples in order to accelerate identification of (emerging) endocrine disruptors.

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“…Like many other estrogenic steroid hormones, the application of ESI in the negative mode is expected to facilitate detection. 30 , 31 …”

Section: Resultsmentioning

confidence: 99%

High-Throughput Effect-Directed Analysis Using Downscaled in Vitro Reporter Gene Assays To Identify Endocrine Disruptors in Surface Water

Zwart¹,

Nio²,

Houtman

et al. 2018

Environ. Sci. Technol.

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“…It was proved that VP4 from Pleurotus ostreatus can decolorize the azo dyes, Orange II, Reactive Black 5, and Amaranth [ 123 ]. The concentrations of Mn 2+ and malonate collectively determine the removal rate of these compounds, corroborating the importance of MnP rather than LiP activity [ 124 ].…”

Section: Linking Oxidative Ability Of Lignin-degrading Enzymes To mentioning

confidence: 79%

Linking Enzymatic Oxidative Degradation of Lignin to Organics Detoxification

Wang

Yao

2018

IJMS

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The major enzymes involved in lignin degradation are laccase, class II peroxidases (lignin peroxidase, manganese peroxidase, and versatile peroxidase) and dye peroxidase, which use an oxidative or peroxidative mechanism to deconstruct the complex and recalcitrant lignin. Laccase and manganese peroxidase directly oxidize phenolic lignin components, while lignin peroxidase and versatile peroxidase can act on the more recalcitrant non-phenolic lignin compounds. Mediators or co-oxidants not only increase the catalytic ability of these enzymes, but also largely expand their substrate scope to those with higher redox potential or more complicated structures. Neither laccase nor the peroxidases are stringently selective of substrates. The promiscuous nature in substrate preference can be employed in detoxification of a range of organics.

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“…Our previous study indicated that VP oxidized not only phenolic compounds but also high-redox potential substrates e.g., anthraquinone dyes Alizarin Blu Black B and Acid Blue 129 [ 23 ]. Moreover, VP had a high application value due to the effective degradation of PHAs [ 25 ], pharmaceuticals and hormones [ 26 ] as well as endocrine disrupting compounds, e.g., Bisphenol A and Triclosan [ 27 , 28 ]. An important parameter that affects not only enzyme’s activity but also its affinity to the substrate is pH.…”

Section: Discussionmentioning

confidence: 99%

Possibility to Biotransform Anthracyclines by Peroxidases Produced by Bjerkandera adusta CCBAS 930 with Reduction of Geno- and Cytotoxicity and Pro-Oxidative Activity

Rybczyńska‐Tkaczyk

Korniłłowicz-Kowalska

Szychowski

2021

Molecules

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The aim of this study was to evaluate the bioremoval mechanism of anthracycline antibiotics by the white-rot fungus B. adusta CCBAS 930. The activity of oxidoreductases and levels of phenolic compounds and free radicals were determined during the biotransformation of anthraquinone antibiotics: daunomycin (DNR) and doxorubicin (DOX) by B. adusta strain CCBAS 930. Moreover, phytotoxicity (Lepidium sativum L.), ecotoxicity (Vibrio fischeri), genotoxicity and cytotoxicity of anthraquinone dyes were evaluated before and after biological treatment. More than 80% and 90% of DNR and DOX were removed by biodegradation (decolorization). Initial solutions of DNR and DOX were characterized by eco-, phyto-, geno- and cytotoxicity. Despite efficient decolorization, secondary metabolites, toxic to bacteria, formed during biotransformation of anthracycline antibiotics in B. adusta CCBAS 930 cultures. DNR and DOX metabolites did not increase reactive oxygen species (ROS) production in human fibroblasts and resazurin reduction. DNR metabolites did not change caspase-3 activity.

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Fostering the action of versatile peroxidase as a highly efficient biocatalyst for the removal of endocrine disrupting compounds

Cited by 31 publications

References 38 publications

High-Throughput Effect-Directed Analysis Using Downscaled in Vitro Reporter Gene Assays To Identify Endocrine Disruptors in Surface Water

High-Throughput Effect-Directed Analysis Using Downscaled in Vitro Reporter Gene Assays To Identify Endocrine Disruptors in Surface Water

Linking Enzymatic Oxidative Degradation of Lignin to Organics Detoxification

Possibility to Biotransform Anthracyclines by Peroxidases Produced by Bjerkandera adusta CCBAS 930 with Reduction of Geno- and Cytotoxicity and Pro-Oxidative Activity

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