Situs inversus totalis (situs inversus with dextrocardia) characterized by transposition of the abdominal viscera and a right-sided heart, is a mirror-image of the normal anatomy. The incidence of situs inversus totalis is 0.01% [1]. In situs inversus totalis, the heart is structurally normal in 90-95%, in contrast to dextrocardia with situs solitus, which involves a high incidence of structural cardiac defects [2]. There have been a number of earlier reports on the antenatal two-dimensional (2D) sonographic diagnosis of situs inversus totalis during pregnancy [3]. However, there has been no report on the three-dimensional (3D) sonographic diagnosis of situs inversus totalis with an inversion mode during pregnancy. This promoted us to present antenatal diagnosis of situs inversus totalis employing 3D/four-dimensional (4D) sonography with an inversion mode.A 29-year-old pregnant Japanese woman, gravida 2, para 1, was referred to our ultrasound clinic because of a right-sided heart and stomach at 24 weeks and 5 days of gestation. 2D sonographic biometric measurements were consistent with 25 weeks of gestation. 2D sonography (Voluson E8, GE Healthcare, Milwaukee, WI, USA) showed mirror-image dextrocardia and the mirror-image arrangement of the stomach, spleen, and liver ( Fig. 1). 3D/ 4D sonography (Voluson E8, GE Healthcare, Milwaukee, WI, USA) with an inversion mode clearly revealed a spatial relationship among mirror-image cardiac structures, the spine, and right-sided stomach (Fig. 2). Especially, the 3D inversion mode facilitated the visualization of the relationship and course of the outflow tracts, thus helping the physician to more effectively understand the spatial relationships between great vessels.A healthy female infant weighing 3,554 g was delivered vaginally at 41 weeks and 4 days of gestation. Echocardiography and radiograph (Fig. 3) confirmed the prenatal findings of situs inversus totalis. The neonate followed a favorable course after delivery.The inversion mode is a volume analysis approach to 3D/4D sonography for the visualization of fluid-filled fetal structures [4]. Anechoic structures, such as the cardiac chambers, vessel lumen, stomach, gallbladder, renal pelvis, and bladder appear echogenic in the rendered images, whereas structures that are normally echogenic before gray scale inversion appear anechoic [5]. Therefore, the 3D/4D inversion mode facilitates the visualization of the relationships and size of the fluid collections, thus helping the sonographer to more effectively understand the spatial relationships between fluid-filled structures [4]. In the present case, the 3D/4D inversion mode was an adjunctive tool to assist in the prenatal understanding of situs inversus with dextrocardia, although conventional 2D sonography can actually suggest the diagnosis.The utility of 3D/4D sonography with an inversion mode in the antenatal diagnosis of fetal cardiac anomalies has been described in several reports [5][6][7][8]. In those investigations, 4D sonography with an inversion mode demonstrate...