Four methods of nondestructive DNA sampling from freshwater pearl mussels <i>Margaritifera margaritifera</i> L. (Bivalvia:Unionoida)

Karlsson, Sten; Larsen, Bjørn Mejdell; Eriksen, Line Birkeland; Hagen, Merethe

doi:10.1899/12-079.1

Cited by 18 publications

(13 citation statements)

References 10 publications

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“…Sampled juveniles had a length of 0.40 ± 0.04 mm (mean ± SD ; range: 0.28–0.56 mm) in June and 1.14 ± 0.13 mm (range: 0.90–1.53 mm) in September. DNA samples from the 52 collected adult mussels were taken on 22 September 2016 by gently collecting material from the visceral mass with a cotton swab (Karlsson, Larsen, Eriksen, & Hagen, ). Cotton swabs were stored in individual tubes containing 600 μl lysis buffer (Qiagen ™ ).…”

Section: Methodsmentioning

confidence: 99%

High levels of multiple paternity in a spermcast mating freshwater mussel

Wacker

Larsen

Jakobsen

et al. 2018

Ecology and Evolution

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Multiple paternity is an important characteristic of the genetic mating system and common across a wide range of taxa. Multiple paternity can increase within‐population genotypic diversity, allowing selection to act on a wider spectre of genotypes, and potentially increasing effective population size. While the genetic mating system has been studied in many species with active mating behavior, little is known about multiple paternity in sessile species releasing gametes into the water. In freshwater mussels, males release sperm into the water, while eggs are retained and fertilized inside the female (spermcast mating). Mature parasitic glochidia are released into the water and attach to the gills of fish where they are encapsulated until settling in the bottom substrate. We used 15 microsatellite markers to detect multiple paternity in a wild population of the freshwater pearl mussel (Margaritifera margaritifera). We found multiple paternity in all clutches for which more than two offspring were genotyped, and numbers of sires were extremely high. Thirty‐two sires had contributed to the largest clutch (43 offspring sampled). This study provides the first evidence of multiple paternity in the freshwater pearl mussel, a species that has experienced dramatic declines across Europe. Previous studies on other species of freshwater mussels have detected much lower numbers of sires. Multiple paternity in freshwater pearl mussels may be central for maintaining genetic variability in small and fragmented populations and for their potential to recover after habitat restoration and may also be important in the evolutionary arms race with their fish host with a much shorter generation time.

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Section: Methodsmentioning

confidence: 99%

High levels of multiple paternity in a spermcast mating freshwater mussel

Wacker

Larsen

Jakobsen

et al. 2018

Ecology and Evolution

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“…(Kawai et al 2004), polyplacophoran molluscs (Ischnochiton spp.) (Palmer et al 2008), freshwater pearl mussels Margaritifera margaritifera (Karlsson et al 2013) and fish (Manta birostris, Oreochromis niloticus) (Kashiwagi et al 2015, Taslima et al 2016.…”

Section: Dna Samplingmentioning

confidence: 99%

Towards more compassionate wildlife research through the 3Rs principles: moving from invasive to non-invasive methods

Zemanova

2020

Wildlife Biology

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“…The D r a f t majority were swabbed with a 6 mm diameter Qiagen buccal swab brush, although smaller unionids such as Villosa fabalis and Strophitus undulatus were swabbed with a 2 mm diameter Microbrush® International in order to minimize the stress to the animals. Swabbed brush tips were cut off and stored in 2 mL screw-cap cryovials with 1 mL 1X TNES-urea buffer at room temperature (Karlsson et al 2013). DNA was extracted from 200 µL of each swab solution that contained 6 mm brushes using a DNeasy Blood and Tissue Kit (Qiagen), following the manufacturer's Supplementary Protocol DY15.…”

Section: R a F Tmentioning

confidence: 99%

Development of species-specific primers with potential for amplifying eDNA from imperilled freshwater unionid mussels

Cho

Morris

Wilson

et al. 2016

Genome

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Environmental DNA (eDNA) is emerging as a potentially powerful tool for inferring species' presence, and hence occupancy, from DNA that is shed into environmental samples such as water. Although eDNA screening has been used to detect DNA from a variety of taxonomic groups, it has not yet been used to identify DNA from species with numerous potentially sympatric confamilial species, a situation that may preclude the development of species-specific markers. There are 41 native freshwater mussel species (Unionidae) in Ontario, Canada. Many of these are potentially sympatric, and 14 species have been formally assessed as endangered, threatened, or special concern. We investigated whether there was sufficient variation within the cytochrome oxidase region (COI) to develop species-specific eDNA markers for at-risk unionids. We developed 32 COI markers for eight unionid species, and tested each of these on the target species plus 29 potentially sympatric unionid taxa. Six of these markers amplified DNA only from the intended target species. We then extracted and amplified mussel eDNA from rearing-tank water samples. We conclude that despite high species diversity, it should be possible to develop eDNA COI markers and screen water samples for habitat occupancy by unionid mussels.

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Four methods of nondestructive DNA sampling from freshwater pearl mussels Margaritifera margaritifera L. (Bivalvia:Unionoida)

Cited by 18 publications

References 10 publications

High levels of multiple paternity in a spermcast mating freshwater mussel

High levels of multiple paternity in a spermcast mating freshwater mussel

Towards more compassionate wildlife research through the 3Rs principles: moving from invasive to non-invasive methods

Development of species-specific primers with potential for amplifying eDNA from imperilled freshwater unionid mussels

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