Four Spanish Isolates of Pear Decline Phytoplasma are Related to Other European Phytoplasmas of the Apple Proliferation Group *

Martín, Robert R.; Carazo, G.; Arribas, Carla Andrea Alonso; Colino, I.; Santiago, R.; Blas, C. de

doi:10.1111/j.1439-0434.2001.tb03881.x

Cited by 2 publications

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the beginning of this study, the phytoplasma-specific rDNA sequence was successfully amplified using PCR with DNA templates prepared from the PDTW-affected pear trees when using the phytoplasma-specific primer pair f1/r1 (Lin & Lin, 1998), but no PCR product was amplified when using the PD phytoplasmaspecific primer pair fPD/rPDS (Lorenz et al, 1995). Since it is known that these primers did not amplify all European strains of the PD agent (Lorenz et al, 1995;Martín et al, 2001), it was not surprising that the PD-specific primer pair fPD/rPDS did not amplify PDTW phytoplasma. In this study, phytoplasma particles were successfully observed by TEM in pears with decline symptoms when using the samples that gave positive PCR results.…”

Section: Discussionmentioning

confidence: 99%

Detection and identification of the phytoplasma associated with pear decline in Taiwan

Liu

Chen²,

Lin

2007

Eur J Plant Pathol

View full text Add to dashboard Cite

Pear decline (PD) is an important phytoplasmal disease that occurs mainly in Europe and North America. In 1994, pear trees exhibiting symptoms typical of PD disease were observed in orchards of central Taiwan. The sequence of 16S rDNA and 16S-23S rDNA intergenic spacer region (ISR) of the causative agent of pear decline in Taiwan (PDTW) were amplified with polymerase chain reaction (PCR) using a DNA template prepared from the diseased leaves. Sequence analysis of 16S rDNA revealed that the PDTW agent was closely related to the phytoplasmas of the apple proliferation group that cause diseases in stone fruits, pear and apple. Consistent with the result of 16S rDNA sequence analysis, sequence analysis of the 16S-23S rDNA ISR and putative restriction site analyses of 16S rDNA and 16S-23S rDNA ISR sequences provided further support for the view that the PDTW phytoplasma causing pear decline in Taiwan may represent a new subgroup of the apple proliferation group. According to the rDNA sequence of PDTW phytoplasma, two specific PCR primer pairs, APf2/L1n and fPD1/rPDS1, were designed in this study for the detection of the etiological agent in pear trees and insect vectors. Based on the sequence analyses of the PCRamplified fragments, two species of pear psyllas, Cacopsylla qianli and Cacopsylla chinensis, were found to carry PDTW phytoplasma.

show abstract

Section: Discussionmentioning

confidence: 99%

Detection and identification of the phytoplasma associated with pear decline in Taiwan

Liu

Chen²,

Lin

2007

Eur J Plant Pathol

View full text Add to dashboard Cite

show abstract

Occurrence, Symptom Expression and Characterization of Phytoplasma Associated with Pear Decline Disease in Catalonia (Spain)

García-Chapa¹,

Laviña²,

Sánchez³

et al. 2003

Journal of Phytopathology

View full text Add to dashboard Cite

A total area of 1500 ha of commercial plots was surveyed to study the extent of pear decline disease and its relative importance in northeastern Spain. A preliminary evaluation indicated that around 7% of the plots had symptoms of the disease. At the same time, pear decline incidence was evaluated in 45 plots, by visual inspection of 500 trees in each plot. In September, the incidence of trees with symptoms ranged from 8 to 59% depending on the cultivar selected. The presence of pear decline (PD) phytoplasma in these plots was confirmed by polymerase chain reaction (PCR) amplification of phytoplasma DNA with universal or group‐specific primers. Restriction fragment length polymorphism analyses also showed the presence of a unique phytoplasma strain. The symptom expression of PD disease in different cultivars was evaluated throughout the year. The relationship between the presence of symptoms and detection of PD by PCR in these cultivars was also studied. Results showed that the nested‐PCR, using specific primers to detect the DNA from PD phytoplasma, is the most accurate method to identify the total percentage of affected trees.

show abstract

Four Spanish Isolates of Pear Decline Phytoplasma are Related to Other European Phytoplasmas of the Apple Proliferation Group *

Cited by 2 publications

References 8 publications

Detection and identification of the phytoplasma associated with pear decline in Taiwan

Detection and identification of the phytoplasma associated with pear decline in Taiwan

Occurrence, Symptom Expression and Characterization of Phytoplasma Associated with Pear Decline Disease in Catalonia (Spain)

Contact Info

Product

Resources

About