Fourier Transform Infrared Spectroscopy

Alben, James O.; Fiamingo, Frank G.

doi:10.1016/b978-0-12-599322-7.50009-9

Cited by 11 publications

(19 citation statements)

References 61 publications

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“…The major absorbance bands are almost identical to those in JS100 (aa3 deletion mutant) ( Table 1). There is a small Fe-CO absorbance peak at 1,965 cm-1 that may be from the aa3-type oxidase, although there is no evidence for Cu-CO absorbance bands from this oxidase. A Western blot of membranes from aerobically and photosynthetically grown Ga and aerobically grown JS100 is shown in Fig.…”

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confidence: 99%

“…When the Fe-CO bond is photolyzed at low temperatures, the CO is trapped in the oxygen binding pocket and binds to the copper (CuB) (11). Both Fe-CO and Cu-CO have distinct ranges of center frequencies for the CO stretching mode (1). Detection of a Cu-CO signal after photolysis at low temperatures is diagnostic of the heme-copper binuclear center.…”

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confidence: 99%

“…This has been demonstrated in beef heart oxidase, in which the relat-ive ratios of the band areas of the ot and ,B forms of the oxidase change between samples but in which the ratio of the total Cu-CO band area to the total Fe-CO band area is always approx'imately the same (11). In spectra of both aerobically and photosynthetically grown R. sphaeroides Ga, the ratio of the total area of the Cu-CO bands to the total area of the Fe-CO bands is the same as that in the beef heart enzyme, indicati'ng 1 b Values were calculated from data in reference 11. stoichiometry (Table 2). If it is assumed that the 1964-cm-1 band is the only Fe-CO absorption peak from the aa3-type oxidase, then the ratio of the total Cu-CO area to that of the 1964-cm-1 band should be similar to the ratio calculated for the beef heart enzyme.…”

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Spectroscopic and genetic evidence for two heme-Cu-containing oxidases in Rhodobacter sphaeroides

et al. 1992

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It has recently become evident that many bacterial respiratory oxidases are members of a superfamily that is related to the eukaryotic cytochrome c oxidase. These oxidases catalyze the reduction of oxygen to water at a heme-copper binuclear center. Fourier transform infrared (FTIR) spectroscopy has been used to examine the heme-copper-containing respiratory oxidases of Rhodobacter sphaeroides Ga. This technique monitors the stretching frequency of CO bound at the oxygen binding site and can be used to characterize the oxidases in situ with membrane preparations. Oxidases that have a heme-copper binuclear center are recognizable by FTIR spectroscopy because the bound CO moves from the heme iron to the nearby copper upon photolysis at low temperature, where it exhibits a diagnostic spectrum. The FTIR spectra indicate that the binuclear center of the R. sphaeroides aa3-type cytochrome c oxidase is remarkably similar to that of the bovine mitochondrial oxidase. Upon deletion of the ctaD gene, encoding subunit I of the aa3-type oxidase, substantial cytochrome c oxidase remains in the membranes of aerobically grown R. sphaeroides. This correlates with a second heme-copper oxidase in these membranes revealed by FTIR spectroscopy. When wild-type R. sphaeroides is grown photosynthetically, the chromatophore membranes lack the aa3-type oxidase but have this second heme-copper oxidase. Subunit I of the heme-copper oxidase superfamily contains the binuclear center. Amino acid sequence alignments show that this subunit is structurally very highly conserved among both eukaryotic and prokaryotic species. The polymerase chain reaction was used to show that the chromosome ofR. sphaeroides contains at least one other gene that is a homolog ofctaD, the gene encoding subunit I of the aa3-type cytochrome c oxidase. Presumably, this second gene codes for subunit I of the second heme-copper oxidase.

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Spectroscopic and genetic evidence for two heme-Cu-containing oxidases in Rhodobacter sphaeroides

et al. 1992

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“…It has many advantages (Table 2), such as the fast and inexpensive performance and the obtaining of characteristic IR fingerprints of each compound, since they are a combination of the vibrational state of each atom [5]. In addition, it can be a quantitative tool in some specific conditions [27]. However, as drawbacks, there is the sample preparation (dried samples required, although some apparatus are prepared for liquid samples, but in this last case, a high concentration of the compounds to analyze is required), a requirement of technique optimization in most cases and interference with water molecules, which strongly absorb and need of a background signal [9].…”

Section: Spectroscopic Techniquesmentioning

confidence: 99%

“…Infrared Spectroscopy. Fourier transformed infrared spectroscopy (FTIR) is a spectroscopic technique based on the measurement of vibrational transitions between different excitation states of molecules [27]. IR radiation is absorbed by molecules with dipoles that oscillate with the same frequency of the incident IR light.…”

Section: Spectroscopic Techniquesmentioning

confidence: 99%

Analytical Methods to Characterize and Purify Polymeric Nanoparticles

Fornaguera

Solans

2018

International Journal of Polymer Science

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Advances in polymeric nanoparticles as novel nanomedicines have opened a new class of diagnostic and therapeutic tools for many diseases. However, although the benchtop research studies in the nanoworld are numerous, their translation to currently marketed products is still limited. This lack of transference can be attributed, among other factors, to problems with nanomedicine characterization. Characterization techniques at the nanoscale could be divided in three categories: characterization of physicochemical properties (e.g., size and surface charge), characterization of nanomaterials interactions with biological components (e.g., proteins from the blood), and analytical characterization and purification methods. Currently available literature of this last group only describes methodologies applied for a specific type of nanomaterial or even methods used for bulk materials, which are not completely applicable to nanomaterials. For this reason, the current review aims to become a scholastic guide for those scientists starting in the nanoworld, giving them a description of analytical characterization techniques aimed to analyze polymers forming nanoparticles and possible forms to purify them before being used in preclinical and clinical applications.

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Biophysics

Rousseau

Jelinski

2003

Digital Encyclopedia of Applied Physics

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Fourier Transform Infrared Spectroscopy

Cited by 11 publications

References 61 publications

Spectroscopic and genetic evidence for two heme-Cu-containing oxidases in Rhodobacter sphaeroides

Spectroscopic and genetic evidence for two heme-Cu-containing oxidases in Rhodobacter sphaeroides

Analytical Methods to Characterize and Purify Polymeric Nanoparticles

Biophysics

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