Fourier-Transform Raman and Fourier-Transform Infrared Spectroscopy (An Investigation of Five Higher Plant Cell Walls and Their Components)

Séné, Christophe; McCann, Maureen C.; Wilson, R. H.; Grinter, R.

doi:10.1104/pp.106.4.1623

Cited by 342 publications

(156 citation statements)

References 20 publications

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“…After 17 dpa, this band remains extremely broad and with almost negligible intensity, following a similar trend as found for the bands located at 2848 and 2916 cm −1 corresponding to CH 2 symmetric stretching. This band is attributed to C=O stretching of saturated alkyl esters associated to pectins [16], and/or waxes [17] and it is strongly influenced by hydrogen bonds [18]. When 51 dpa G. hirsutum fibers grown under the same conditions are treated with NaOH/ ethylenediaminetetraacetic acid/sodium dodecyl sulfate (EDTA/SDS) for 1 h at 80 • C (scouring), this band completely disappears [7] supporting the hypothesis of a hydrophobic nature of the components (waxes or alkylated pectins).…”

Section: Ft-ir Atr Characterization Of G Hirsutum Fibers Developmentmentioning

confidence: 99%

Structural Evolution of Gossypium hirsutum Fibers Grown under Greenhouse and Hydroponic Conditions

Natálio

Maria

2018

Fibers

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Abstract:Cotton is the leading fiber source in the textile industry and one of the world's most important crops. Despite its economic interest, cotton culture exerts an enormous pressure on natural resources (land and water) and has a negative impact on the environment (abuse of pesticides). Thus, alternative cotton growing methods are urged to be implemented. Recently, we have demonstrated that Gossypium hirsutum ("Upland" cotton) can be grown in a greenhouse (controlled conditions) and hydroponically. Here we report on the elucidation of the structural changes of the Gossypium hirsutum fibers during maturation grown [10,14,17,20, 36 and 51 days post anthesis (dpa)] under a greenhouse and hydroponically, by means of scanning electron microscopy (SEM), Fourier transform infrared spectroscopy with attenuated total reflectance (FT-IR ATR) and thermal gravimetric analysis/differential scanning calorimetry (TGA/DSC). The transition from primary to secondary cell wall growth occurs between 17 and 20 dpa-similarly to the soil-based cultures. However, this new cotton culture offers an advantageous pesticide and soil-free all year-round closed system with efficient water use yielding standardized mature fibers with improved properties (maturity, strength, length, whiteness).

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Section: Ft-ir Atr Characterization Of G Hirsutum Fibers Developmentmentioning

confidence: 99%

Structural Evolution of Gossypium hirsutum Fibers Grown under Greenhouse and Hydroponic Conditions

Natálio

Maria

2018

Fibers

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“…The first corresponded to esters. The second area was more difficult to associate with the differences with cell wall compo- nents, due to high number of vibration modes in polysaccharide links (Sene et al, 1994). To elucidate differences among these spectra, a set of principal components analysis (PCA) was performed.…”

Section: Analysis Of Cell Wall Components Links By Ftirmentioning

confidence: 99%

Xyloglucan endotransglucosylase and cell wall extensibility

Miedes

Zarra

Hoson

et al. 2011

Journal of Plant Physiology

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SUMMARY Keywords:Cell wall. Extensibility. Growth. Solanum lycopersicum L. cv. Money Maker. Xyloglucan Transgenic tomato hypocotyls with altered levels of an XTH gene were used to study how XET activity could affect the hypocotyl growth and cell wall extensibility. Transgenic hypocotyls showed significant over-expression (line 13) or co-suppression (line 33) of the SIXTH! in comparison with the wild type, with these results being correlated with the results on specific soluble XET activity, suggesting that SIXTH! translates mainly for a soluble XET isoenzyme. A relationship between XET activity and cell wall extensibility was found, and the highest total extensibility was located in the apical hypocotyl segment of the over-expressing SIXTH! line, where the XET-specific activity and hypocotyl growth were also highest compared with the wild line. Also, in the co-suppression SIXTH! line, total extensibility values were lower than in the wild type line. The study of linkages between cell wall polysaccharides by FTIR showed that hypocotyls over-expressing SIXTH! and having a higher XET-specific activity, were grouped away from the wild line, indicating that the linkages between pectins and between cellulose and xyloglucans might differ. These results suggested that the action of the increased XET activity in the transgenic line could be responsible for the cell wall structural changes, and therefore, alter the cell wall extensibility. On the other hand, results on xyloglucan oligosaccharides composition of the xyloglucan by MALDITOF-MS showed no differences between lines, indicating that the xyloglucan structure was not affected by the XET action. These results provide evidences that XTHs from group I are involved mainly in the restructuring of the cell wall during growth and development, but they are not the limiting factor for plant growth.

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“…C-C). In practice, carbohydrates and lignin give both IR absorption and Raman spectra, and many of their vibrational modes appear in both [4,49,52]. Absorption spectra are easier to collect using simpler instrumentation, but sometimes as with lignin, the Raman spectra are more useful [1,3].…”

Section: Vibrational (Infrared Absorption and Raman) Spectroscopymentioning

confidence: 99%

Macromolecular biophysics of the plant cell wall: Concepts and methodology

Jarvis

McCann

2000

Plant Physiology and Biochemistry

106

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-Plant cell walls provide form and mechanical strength to the living plant, but the relationship between their complex architecture and their remarkable ability to withstand external stress is not well understood. Primary cell walls are adapted to withstand tensile stresses while secondary cell walls also need to withstand compressive stresses. Therefore, while primary cell walls can with advantage be flexible and elastic, secondary cell walls must be rigid to avoid buckling under compressive loads. In addition, primary cell walls must be capable of growth and are subjected to cell separation forces at the cell corners. To understand how these stresses are resisted by cell walls, it will be necessary to find out how the walls deform internally under load, and how rigid are specific constituents of each type of cell wall. The most promising spectroscopic techniques for this purpose are solid-state nuclear magnetic resonance (NMR), and Fourier-transform infrared (FTIR) and Raman microscopy. By NMR relaxation experiments, it is possible to probe thermal motion in each cell-wall component. Novel adaptations of FTIR and Raman spectroscopy promise to allow mechanical stress and strain upon specific polymers to be examined in situ within the cell wall. © 2000 Éditions scientifiques et médicales Elsevier SAS Cellulose / growth / mobility / pectin CP, cross-polarisation / FT, Fourier-transform / IR, infrared / MAS, magic-angle spinning / NMR, nuclear magnetic resonance / T 2 , spin-spin relaxation time constant

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Fourier-Transform Raman and Fourier-Transform Infrared Spectroscopy (An Investigation of Five Higher Plant Cell Walls and Their Components)

Cited by 342 publications

References 20 publications

Structural Evolution of Gossypium hirsutum Fibers Grown under Greenhouse and Hydroponic Conditions

Structural Evolution of Gossypium hirsutum Fibers Grown under Greenhouse and Hydroponic Conditions

Xyloglucan endotransglucosylase and cell wall extensibility

Macromolecular biophysics of the plant cell wall: Concepts and methodology

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