Fox grape cv. Bordô (Vitis labrusca L.) and grapevine cv. Chardonnay (Vitis vinifera L.) cultivated in vitro under different carbohydrates, amino acids and 6-Benzylaminopurine levels

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Section: Lavandula Angustifolia Mill (Syn L Officinalismentioning

confidence: 99%

Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.

Machado

Silva

Biasi

et al. 2014

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“…Differences in the gene expression are caused by the somaclonal variation in the explants during the sub-cultures (Kota et al 2006;Lima et al 2008). On the other hand, this somaclonal variation can be a useful source of new variation for genetic breeding, mainly for the selection of cell lines with higher yield (Carvalho et al 2013). The a appearance of the calli ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents

Rodrigues

Festucci-Buselli

Silva

et al. 2014

“…Adverse effect of prolonged in vitro culture is reported by many workers (Jureti andJelaska 1991, Choi et al 2001). On the other hand, somaclonal variation can arise, which is a useful source of new variation for genetic breeding (Carvalho et al 2013). Germination of somatic embryos and its conversion into plantlets are crucial steps for the successful plant regeneration.…”

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant

Sharmin

Alam

Sheikh

et al. 2014

In this work, plant regeneration via somatic embryogenesis was achieved from leaf and internode derived callus of Wedelia calendulacea, an endangered medicinal plant. Primary callus was induced by culturing leaf disc and internode explant on Murashige and Skoog medium supplemented with 2.0 mg L-1 of 2,4-D under light condition. Transfer of embryogenic callus on a reduced concentration of 2,4-D facilitated somatic embryo development while calluses remained unorganized at the same 2,4-D level. A histological analysis confirmed somatic embryo by revealing the presence of a closed vascular system in the developing embryos and lack of a vascular connection with surrounding callus tissues. Somatic embryos germinated into plantlets upon transfer on MS medium containing 1.0 mg L-1 BAP plus 0.5 mg L-1 GA 3. Plantlets were acclimatized successfully and survived under soil condition. This is the first on somatic embryogenesis of W. calendulacea. This result could facilitate genetic transformation of this important medicinal plant.