FOXM1 expression correlates with tumor invasion and a poor prognosis of colorectal cancer

Chen, Xiaoyuan; Zhu, Ziman; Chen, Longbang; Wang, Jinghua; Su, Qi; Yang, Jin‐Lin; Lin, Yunfeng; Xue, Lijun; Liu, Xiao-Bei; Mo, Xiao-Bei

doi:10.1016/j.acthis.2012.01.002

Cited by 83 publications

(76 citation statements)

References 23 publications

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“…Previously, we have shown that FOXM1 over-expression is a molecular marker predicting increased invasive/metastatic potential of CRC and a poorer prognosis [15]. However, the molecular mechanism involved in FOXM1 overexpression in CRC is not clear.…”

Section: Resultsmentioning

confidence: 99%

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MicroRNA-149 Suppresses Colorectal Cancer Cell Migration and Invasion by Directly Targeting Forkhead Box Transcription Factor FOXM1

Liu

Mao

et al. 2015

Cell Physiol Biochem

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Background/Aims: The aim of this study is to investigate the clinicopathological and prognostic values of miR-149 expression and its roles in colorectal cancer (CRC) progression. Methods: qRT-PCR was performed to detect miR-149 expression in CRC cell lines or tissues. Also, the clinical significance of miR-149 expression was investigated. The study further explored whether miR-149 inhibits migration and invasion of CRC cells by targeting the mammalian Forkhead Box M1 (FOXM1). Results: miR-149 was significantly downregulated in CRC tissues, and low miR-149 expression was observed to be significantly correlated with lymph node or distant metastasis and advanced TNM stage of CRC patients. Patients with low miR-149 expression showed poorer prognosis than those with high miR-149 expression, and multivariate analyses indicated that status of miR-149 expression might be an independent prognostic factor. Gain- and loss - of - function assays indicated that miR-149 significantly inhibited growth, migration and invasion of CRC cells by targeting FOXM1. Furthermore, FOXM1 was significantly uiregulated in CRC tissues and inversely correlated with miR-149 expression. Conclusions: mR-149 was an independent prognostic factor and could inhibit migration and invasion of CRC cells, at least partially by targeting FOXM1.

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Section: Resultsmentioning

confidence: 99%

“…The small hairpin RNA (shRNA) plasmid vector targeting FOXM1 (pSil/shFOXM1) and control vector (pSil/shcontrol) were successfully constructed previously [15]. The open reading frame of FOXM1 that was generated by PCR was then inserted into the pEGFP-C1 expression vector which was named pEGFP/FOXM1.…”

Section: Methodsmentioning

confidence: 99%

MicroRNA-149 Suppresses Colorectal Cancer Cell Migration and Invasion by Directly Targeting Forkhead Box Transcription Factor FOXM1

Liu

Mao

et al. 2015

Cell Physiol Biochem

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“…Xia et al found that a high level of expression of FoxM1 was significantly correlated with clinical staging (P = 0.00), lymph node metastasis (P = 0.01), and histological differentiation (P = 0.02) in pancreatic ductal adenocarcinoma (Xia et al, 2012). Chu et al revealed that high FoxM1 expression was closely correlated with the presence of lymph node metastasis, incidence of liver metastasis, and advanced TNM stage in colorectal cancer (Chu et al, 2012). But Liu et al reported that FoxM1 was only correlated with TNM stage (P=0.01), which is a little different from our findings.…”

Section: Discussionmentioning

confidence: 99%

Involvement of FoxM1 in Non-Small Cell Lung Cancer Recurrence

Wu²,

Wang³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Background: Predictive biomarkers for lung cancer recurrence after curative tumor resection remain unclear. This study set out to assess the role of FoxM1 in the recurrence of non-small cell lung cancer. Methods: Immunohistochemistry for FoxM1 expression was performed on paraffin-embedded tumor tissues from 165 NSCLC patients. Association of FoxM1 expression with clinicopathological parameters and disease free survival were evaluated. Results: Our results indicated FoxM1 expression to be significantly associated with poorer tissue differentiation (P =0.03), higher TNM stage (P <0.01), lymph node metastasis (P <0.01), advanced tumor stage (P <0.01), and poorer disease free survival (P <0.01). Multivariable analysis showed that FoxM1 expression increased the hazard of recurrence (hazard ratio= 1.96, 95% CI, 1.04-3.17, P <0.05), indicating that FoxM1 is an independent and significant predictor of lung cancer recurrence. Conclusion: Therefore, FoxM1 is an independent risk factor for recurrence of NSCLC. Elevated FoxM1 expression could be used as an indicator of poor disease free survival.

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“…The small hairpin RNA (shRNA) plasmid vector targeting FOXM1 (pSil/shFOXM1) and control vector (pSil/shcontrol) were successfully constructed previously [12]. The recombinant vectors were confirmed by the digestion analysis of restriction endonuclease and DNA sequencing.…”

Section: Transfection Of Mirna Mimics Inhibitors or Plasmid Vectorsmentioning

confidence: 99%

MicroRNA-149 Increases the Sensitivity of Colorectal Cancer Cells to 5-Fluorouracil by Targeting Forkhead Box Transcription Factor FOXM1

Liu

Xie

Mao

et al. 2016

Cell Physiol Biochem

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Background/Aims: Previously, we have shown that microRNA (miR)-149 suppresses the migration and invasion of colorectal cancer (CRC) cells by targeting forkhead box transcription factor (FOXM1). However, the roles of miR-149 in the chemoresistance of CRC cells to 5-Fluorouracil (5-FU) is unclear. The aim of this study is to investigate whether miR-149 targets FOXM1 to regulate the 5-FU resistance of CRC. Methods: The qRT-PCR assay was performed to detect the expression of miR-149 in 5-FU-resistant CRC cells (HCT-8/5-FU and LoVo/5-FU) and their parental CRC cells (HCT-8 and LoVo). Also, the effects of miR-149 expression on the sensitivity of CRC cells to 5-FU were determined by gain- and loss-of-function assays. Finally, whether miR-149 regulates the 5-FU resistance of CRC cells by targeting the mammalian Forkhead Box M1 (FOXM1) was investigated. Results: The expression of miR-149 was significantly downregulated in 5-FU-resistant CRC cells in comparison with their parental CRC cells. Re-expression of miR-149 could enhance the 5-FU sensitivity of 5-FU-resistant CRC cells by increasing 5-FU-inducing apoptosis, while downregulation of miR-149 could decrease the 5-FU sensitivity of parental CRC cells by decreasing 5-FU-inducing apoptosis. In addition, the luciferase assay indicated that miR-149 could bind to the 3'-UTR sequence of FOXM1 mRNA. The silencing of FOXM1 could mimic the effect of miR-149 upregulation on the 5-FU resistance of 5-FU-resistant CRC cells. Furthermore, the expression of miR-149 in the 5-FU-responding CRC tissues was significantly higher than that in the non-responding tissues and inversely correlated with FOXM1 mRNA level. Conclusions: MiR-149 reverses the resistance of CRC cells to 5-FU by directly targeting FOXM1. Thus, targeting miR-149/FOXM1 signaling will be a potential strategy in the treatment of 5-FU-chemoresistant CRC.

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FOXM1 expression correlates with tumor invasion and a poor prognosis of colorectal cancer

Cited by 83 publications

References 23 publications

MicroRNA-149 Suppresses Colorectal Cancer Cell Migration and Invasion by Directly Targeting Forkhead Box Transcription Factor FOXM1

MicroRNA-149 Suppresses Colorectal Cancer Cell Migration and Invasion by Directly Targeting Forkhead Box Transcription Factor FOXM1

Involvement of FoxM1 in Non-Small Cell Lung Cancer Recurrence

MicroRNA-149 Increases the Sensitivity of Colorectal Cancer Cells to 5-Fluorouracil by Targeting Forkhead Box Transcription Factor FOXM1

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