FoxO transcription factors 1 regulate mouse preimplantation embryo development

Kuşçu, Nilay; Güngör‐Ordueri, Nazlı Ece; Sözen, Berna; Adiguzel, Dileyra; Çelik-Özenci, Çiler

doi:10.1007/s10815-019-01555-1

Cited by 18 publications

(10 citation statements)

References 54 publications

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“…Finally, the FoxO signaling pathway involves a family of transcription factors that regulates the expression of genes implicated in apoptosis, cell-cycle control, glucose metabolism, oxidative stress resistance, and longevity. Knockdown of FoxO1, FoxO2 and FoxO4 genes has been shown to impair mouse preimplantation embryonic development ( 66 ). In the current study, it is difficult to decipher the collective actions of the differentially expressed miRNAs, but from previous studies it is likely that these miRNAs have an effect on early embryo development, prior to embryonic genome activation ( 23 , 67 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed mRNAs and miRNAs in spermatozoa of bulls of varying fertility

et al. 2022

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Bulls used in artificial insemination, with apparently normal semen quality, can vary significantly in their field fertility. This study aimed to characterize the transcriptome of spermatozoa from high (HF) and low (LF) fertility bulls at the mRNA and miRNA level in order to identify potential novel markers of fertility. Holstein-Friesian bulls were assigned to either the HF or LF group (n = 10 per group) based on an adjusted national fertility index from a minimum of 500 inseminations. Total RNA was extracted from a pool of frozen-thawed spermatozoa from three different ejaculates per bull, following which mRNA-seq and miRNA-seq were performed. Six mRNAs and 13 miRNAs were found differentially expressed (P < 0.05, FC > 1.5) between HF and LF bulls. Of particular interest, the gene pathways targeted by the 13 differentially expressed miRNAs were related to embryonic development and gene expression regulation. Previous studies reported that disruptions to protamine 1 mRNA (PRM1) had deleterious consequences for sperm chromatin structure and fertilizing ability. Notably, PRM1 exhibited a higher expression in spermatozoa from LF than HF bulls. In contrast, Western Blot analysis revealed a decrease in PRM1 protein abundance for spermatozoa from LF bulls; this was not associated with increased protamine deficiency (measured by the degree of chromatin compaction) or DNA fragmentation, as assessed by flow cytometry analyses. However, protamine deficiency was positively and moderately correlated with the percentage of spermatozoa with DNA fragmentation, irrespective of fertility group. This study has identified potential biomarkers that could be used for improving semen quality assessments of bull fertility.

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Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed mRNAs and miRNAs in spermatozoa of bulls of varying fertility

et al. 2022

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“…The expression of antioxidant genes in the FoxO signaling pathway was analyzed to evaluate the quality of BLs cultured with ASC-CM or AMSC-CM. FoxO transcription factors modulate various cellular functions, including differentiation, growth, metabolism, and apoptosis [ 46 ]. These factors predominantly regulate the oxidative stress response by controlling the expression of manganese-dependent SOD (SOD2), catalase, and GPx1 that constitute the primary defense mechanism against ROS [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…These factors predominantly regulate the oxidative stress response by controlling the expression of manganese-dependent SOD (SOD2), catalase, and GPx1 that constitute the primary defense mechanism against ROS [ 47 ]. FoxO is considered as a therapeutic target for infertility and is critical for the preimplantation embryo development in mice [ 46 ]. Specifically, among the mammalian FoxO family, FoxO1 and FoxO3 are key players in female reproductive processes [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Anti-Oxidative Effect of Human Adipose- and Amniotic Membrane-Derived Mesenchymal Stem Cell Conditioned Medium on Mouse Preimplantation Embryo Development

Kim

et al. 2021

Antioxidants

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Oxidative stress is a major cause of damage to the quantity and quality of embryos produced in vitro. Antioxidants are usually supplemented to protect embryos from the suboptimal in vitro culture (IVC) environment. Amniotic membrane-derived mesenchymal stem cells (AMSC) have emerged as a promising regenerative therapy, and their paracrine factors with anti-oxidative effects are present in AMSC conditioned medium (CM). We examined the anti-oxidative potential of human AMSC-CM treatment during IVC on mouse preimplantation embryo development and antioxidant gene expression in the forkhead box O (FoxO) pathway. AMSC-CM (10%) was optimal for overall preimplantation embryo developmental processes and upregulated the expression of FoxOs and their downstream antioxidants in blastocysts (BL). Subsequently, compared to adipose-derived mesenchymal stem cell (ASC)-CM, AMSC-CM enhanced antioxidant gene expression and intracellular GSH levels in the BL. Total antioxidant capacity and SOD activity were greater in AMSC-CM than in ASC-CM. Furthermore, SOD and catalase were more active in culture medium supplemented with AMSC-CM than in ASC-CM. Lastly, the anti-apoptotic effect of AMSC-CM was observed with the regulation of apoptosis-related genes and mitochondrial membrane potential in BL. In conclusion, the present study established AMSC-CM treatment at an optimal concentration as a novel antioxidant intervention for assisted reproduction.

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“…Knockout/knockdown of some redoxsensing/regulating transcription factors can also disrupt development at an early stage. Knockdown of FoxO proteins (FoxO1 , FoxO3 and FoxO4) in mice impairs preimplantation embryo development [132]: The majority of the FoxO knockdown embryos arrest at the 2-cell stage, and blastocyst formation decreases from ~70% to ~25%. ROS in these embryos is elevated 3-4 fold, and in the 2-cell arrested embryos there are 1.5-3 fold increases in Fasl and cleaved caspase 3 (responsible for apoptosis) and p53 and p21 (responsible for cell-cycle arrest) [132].…”

Section: The Preimplantation Embryomentioning

confidence: 99%

Redox Regulation and Oxidative Stress in Mammalian Oocytes and Embryos Developed In Vivo and In Vitro

Hardy

Day

Morris

2021

IJERPH

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Oocytes and preimplantation embryos require careful regulation of the redox environment for optimal development both in vivo and in vitro. Reactive oxygen species (ROS) are generated throughout development as a result of cellular metabolism and enzyme reactions. ROS production can result in (i) oxidative eustress, where ROS are helpful signalling molecules with beneficial physiological functions and where the redox state of the cell is maintained within homeostatic range by a closely coupled system of antioxidants and antioxidant enzymes, or (ii) oxidative distress, where excess ROS are deleterious and impair normal cellular function. in vitro culture of embryos exacerbates ROS production due to a range of issues including culture-medium composition and laboratory culture conditions. This increase in ROS can be detrimental not only to assisted reproductive success rates but can also result in epigenetic and genetic changes in the embryo, resulting in transgenerational effects. This review examines the effects of oxidative stress in the oocyte and preimplantation embryo in both the in vivo and in vitro environment, identifies mechanisms responsible for oxidative stress in the oocyte/embryo in culture and approaches to reduce these problems, and briefly examines the potential impacts on future generations.

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FoxO transcription factors 1 regulate mouse preimplantation embryo development

Cited by 18 publications

References 54 publications

Identification of differentially expressed mRNAs and miRNAs in spermatozoa of bulls of varying fertility

Identification of differentially expressed mRNAs and miRNAs in spermatozoa of bulls of varying fertility

Comparison of Anti-Oxidative Effect of Human Adipose- and Amniotic Membrane-Derived Mesenchymal Stem Cell Conditioned Medium on Mouse Preimplantation Embryo Development

Redox Regulation and Oxidative Stress in Mammalian Oocytes and Embryos Developed In Vivo and In Vitro

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